The Genetic Map of Artemisia annua L. Identifies Loci Affecting Yield of the Antimalarial Drug Artemisinin

Graham, Ian A.; Besser, Katrin; Blumer, Susan; Branigan, Caroline A.; Czechowski, Tomasz; Elias, Luisa; Guterman, Inna; Harvey, David; Isaac, Peter G.; Khan, Awais M.; Larson, Tony R.; Li, Yi; Pawson, Tanya; Penfield, Teresa; Rae, Anne M.; Rathbone, Deborah A.; Reid, Sonja; Ross, Joe; Smallwood, Margaret F.; Segura, Vincent; Townsend, Theresa; Vyas, Darshna; Winzer, Thilo; Bowles, Dianna

doi:10.1126/science.1182612

Cited by 348Genetic Mapping and Diversity in Plants and AnimalsPlant biochemistry and biosynthesisGenetic and phenotypic traits in livestock

The Genetic Map of Artemisia annua L. Identifies Loci Affecting Yield of the Antimalarial Drug Artemisinin

Graham, Ian A., Besser, Katrin, Blumer, Susan, Branigan, Caroline A., Czechowski, Tomasz, Elias, Luisa, Guterman, Inna, Harvey, David +16 more·2010·Science

DOI 10.1126/science.1182612

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Abstract

The Art of Artemisia: As the malaria parasite, which is transmitted through mosquito vectors, develops resistance, previously useful control mechanisms are beginning to fail. Combination therapies based on the plant product artemisinin are a promising alternative. Graham et al. (p. 328 ; see the Perspective by Milhous and Weina ) have now developed a genetic map of the plant Artemisia annua from which artemisinin is derived. The results lay the foundation for improving agricultural productivity of this natural product, which is becoming increasingly important in the fight against malaria.

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The DXR locus co-localizes with the QTL affecting concentration and yield of artemisinin.
“The DXR locus co-localizes with the QTL affecting concentration and yield of artemisinin (Graham et al. 2010).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Graham et al. 2010
Glandular secretory trichomes express multiple members of gene families, encoding enzymes of specialised metabolism including terpene synthases and cytochrome P450 oxidases.
“it is known that glandular secretory trichomes express multiple members of gene families, encoding enzymes of specialised metabolism including terpene synthases and cytochrome P450 oxidases (16, 19, 23).”
From Artemisia annua mutant impaired in artemisinin synthesis demonstrates importance of nonenzymatic conversion in terpenoid metabolism (2016) · cites this paper as [19]
Modern molecular breeding has succeeded in improving A. annua, creating hybrids reaching artemisinin yields of 1.4% dry leaf biomass in commercial field trials.
“modern molecular breeding has succeeded in improving A. annua (19), creating hybrids reaching artemisinin yields of 1.4% dry leaf biomass in commercial field trials (20)”
From Artemisia annua mutant impaired in artemisinin synthesis demonstrates importance of nonenzymatic conversion in terpenoid metabolism (2016) · cites this paper as [19]
The CNAP Artemisia Research Project screened more than 23,000 parental lines and developed about 768 hybrid lines, of which 268 most promising were selected for field trials.
“They screened more than 23,000 parental lines for the desired traits and developed about 768 hybrid lines, of which the 268 most promising hybrids were selected for field trials (Graham et al. 2010; Townsend et al. 2013).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Graham et al. 2010

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Authors: Graham, I. A., Besser, K., Blumer, S., Branigan, C. A., Czechowski, T., Elias, L., Guterman, I., Harvey, D., Isaac, P. G., Khan, A. M., Larson, T. R., Li, Y., Pawson, T., Penfield, T., Rae, A. M., Rathbone, D. A., Reid, S., Ross, J., Smallwood, M. F., Segura, V., Townsend, T., Vyas, D., Winzer, T., & Bowles, D.
Affiliations: University of York (GB)
Norwich Research Park (GB)
Corresponding author: Graham, Ian A.
Bowles, Dianna
ORCID: Graham, Ian A.
Besser, Katrin
Czechowski, Tomasz
Harvey, David
Larson, Tony R.
Li, Yi
Segura, Vincent
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2010-01-15
Volume: 327
Issue: 5963
Pages: 328-331
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1182612
OpenAlex: W2075579534
Cited by: 348
References: 14
Citations by year: 2026: 6 · 2025: 10 · 2024: 11 · 2023: 15 · 2022: 17 · 2021: 20 · 2020: 10 · 2019: 22 · 2018: 25 · 2017: 27 · 2016: 31 · 2015: 24 · 2014: 29 · 2013: 29 · 2012: 33
FWCI: 18.15
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
HAL (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
Keywords: Artemisia annua, artemisinin, genetic linkage map, linkage groups, quantitative trait loci, artemisinin yield, malaria treatment, plant breeding, transcriptome, hybridization, genetic variation
MeSH terms: Antimalarials, Chromosome Mapping, Crosses, Genetic, Humans, Malaria, Mutation, Phenotype, Genes, Plant, Sequence Analysis, DNA, DNA, Complementary, Artemisia, Polymorphism, Single Nucleotide, Gene Expression Profiling, Artemisinins, Quantitative Trait Loci, Genetic Association Studies

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The Genetic Map of Artemisia annua L. Identifies Loci Affecting Yield of the Antimalarial Drug Artemisinin

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