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Elucidation of the RNA Recognition Code for Pentatricopeptide Repeat Proteins Involved in Organelle RNA Editing in Plants

Open access
Yagi, Yusuke, Hayashi, Shimpei, Kobayashi, Keiko, Hirayama, Takashi, Nakamura, Takahiro·2013·PLoS ONE

Abstract

Pentatricopeptide repeat (PPR) proteins are eukaryotic RNA-binding proteins that are commonly found in plants. Organelle transcript processing and stability are mediated by PPR proteins in a gene-specific manner through recognition by tandem arrays of degenerate 35-amino-acid repeating units, the PPR motifs. However, the sequence-specific RNA recognition mechanism of the PPR protein remains largely unknown. Here, we show the principle underlying RNA recognition for PPR proteins involved in RNA editing. The distance between the PPR-RNA alignment and the editable C was shown to be conserved. Amino acid variation at 3 particular positions within the motif determined recognition of a specific RNA in a programmable manner, with a 1-motif to 1-nucleotide correspondence, with no gap sequence. Data from the decoded nucleotide frequencies for these 3 amino acids were used to assign accurate interacting sites to several PPR proteins for RNA editing and to predict the target site for an uncharacterized PPR protein.

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Metadata

Authors
Yagi, Y., Hayashi, S., Kobayashi, K., Hirayama, T., & Nakamura, T.
Venue
PLoS ONE
Published
2013-03-05
Volume
8
Issue
3
Pages
e57286
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2042792861
Cited by
293
References
2
FWCI
13.58
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Elucidation of the RNA Recognition Code for Pentatricopeptide Repeat Proteins Involved in Organelle RNA Editing in Plants. https://app.cochat.ai/papers/10.1371/journal.pone.0057286
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Yagi, Y., Hayashi, S., Kobayashi, K., Hirayama, T., & Nakamura, T. (2013). Elucidation of the RNA Recognition Code for Pentatricopeptide Repeat Proteins Involved in Organelle RNA Editing in Plants. PLoS ONE. https://doi.org/10.1371/journal.pone.0057286

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