Cited by 341Cancer Cells and MetastasisCellular Mechanics and Interactions3D Printing in Biomedical Research

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Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines

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Luca, Anna C., Mersch, Sabrina, Deenen, René, Schmidt, Stephan, Messner, Isabelle, Schäfer, Karl-Ludwig, Baldus, Stephan E., Huckenbeck, Wolfgang, Piekorz, Roland P., Knoefel, Wolfram T., Krieg, Andreas, Stoecklein, Nikolas H.·2013·PLoS ONE

DOI 10.1371/journal.pone.0059689PMC PMC3608563

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Abstract

Three-dimensional (3D) tumor cell cultures grown in laminin-rich-extracellular matrix (lrECM) are considered to reflect human tumors more realistic as compared to cells grown as monolayer on plastic. Here, we systematically investigated the impact of ECM on phenotype, gene expression, EGFR signaling pathway, and on EGFR inhibition in commonly used colorectal cancer (CRC) cell lines. LrECM on-top (3D) culture assays were performed with the CRC cell lines SW-480, HT-29, DLD-1, LOVO, CACO-2, COLO-205 and COLO-206F. Morphology of lrECM cultivated CRC cell lines was determined by phase contrast and confocal laser scanning fluorescence microscopy. Proliferation of cells was examined by MTT assay, invasive capacity of the cell lines was assayed using Matrigel-coated Boyden chambers, and migratory activity was determined employing the Fence assay. Differential gene expression was analyzed at the transcriptional level by the Agilent array platform. EGFR was inhibited by using the specific small molecule inhibitor AG1478. A specific spheroid growth pattern was observed for all investigated CRC cell lines. DLD-1, HT-29 and SW-480 and CACO-2 exhibited a clear solid tumor cell formation, while LOVO, COLO-205 and COLO-206F were characterized by forming grape-like structures. Although the occurrence of a spheroid morphology did not correlate with an altered migratory, invasive, or proliferative capacity of CRC cell lines, gene expression was clearly altered in cells grown on lrECM as compared to 2D cultures. Interestingly, in KRAS wild-type cell lines, inhibition of EGFR was less effective in lrECM (3D) cultures as compared to 2D cell cultures. Thus, comparing both 2D and 3D cell culture models, our data support the influence of the ECM on cancer growth. Compared to conventional 2D cell culture, the lrECM (3D) cell culture model offers the opportunity to investigate permanent CRC cell lines under more physiological conditions, i.e. in the context of molecular therapeutic targets and their pharmacological inhibition.

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Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Integrins: Versatility, modulation, and signaling in cell adhesion
Hynes, R. O. (1992) · Cell · doi:10.1016/0092-8674(92)90115-s · Cited by 9,857
Wild-Type BRAF Is Required for Response to Panitumumab or Cetuximab in Metastatic Colorectal Cancer
Di Nicolantonio, F., Martini, M., Molinari, F., Sartore-Bianchi, A., Arena, S., Saletti, P., De Dosso, S., Mazzucchelli, L., Frattini, M., Siena, S., et al. (2008) · Journal of Clinical Oncology · doi:10.1200/jco.2008.18.0786 · Cited by 1,591 · cited 1×
“KRAS and BRAF mutational status of patients is usually assessed before EGFR treatment on the basis that only patients exhibiting BRAF and KRAS wild type profit from treatments with anti-EGFR therapies [29,30].”
Background
Basement membrane complexes with biological activity
Kleinman, H. K., McGarvey, M. L., Hassell, J. R., Star, V. L., Cannon, F. B., Laurie, G. W., & Martin, G. R. (1986) · Biochemistry · doi:10.1021/bi00350a005 · Cited by 1,502
Three-dimensional culture models of normal and malignant breast epithelial cells
Lee, G. Y., Kenny, P. A., Lee, E. H., & Bissell, M. J. (2007) · Nature Methods · doi:10.1038/nmeth1015 · Cited by 1,336
Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma
Kleinman, H. K., McGarvey, M. L., Liotta, L. A., Robey, P. G., Tryggvason, K., & Martin, G. R. (1982) · Biochemistry · doi:10.1021/bi00267a025 · Cited by 1,231
Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells.
PETERSEN, O. W., Rønnov‐Jessen, L., Howlett, A. R., & Bissell, M. J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.19.9064 · Cited by 1,096
The morphologies of breast cancer cell lines in three-dimensional assays correlate with \ntheir profiles of gene expression
Kenny, P. A., Lee, G. Y., Myers, C. A., Neve, R. M., Semeiks, J. R., Spellman, P. T., Lorenz, K., Lee, E. H., Barcellos‐Hoff, M. H., PETERSEN, O. W., et al. (2008) · eScholarship (California Digital Library) · Cited by 1,039
Oncogenic Activation of the RAS/RAF Signaling Pathway Impairs the Response of Metastatic Colorectal Cancers to Anti–Epidermal Growth Factor Receptor Antibody Therapies
Benvenuti, S., Sartore-Bianchi, A., Di Nicolantonio, F., Zanon, C., Moroni, M., Veronese, S., Siena, S., & Bardelli, A. (2007) · Cancer Research · doi:10.1158/0008-5472.can-06-4158 · Cited by 830
β4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium
Weaver, V. M., Lelièvre, S. A., Lakins, J. N., Chrenek, M. A., Jones, J., Giancotti, F. G., Werb, Z., & Bissell, M. J. (2002) · Cancer Cell · doi:10.1016/s1535-6108(02)00125-3 · Cited by 794

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Show all 31 references

Research works printed but not linked: 1

PD Yurchenco, 1992, Laminin forms an independent network in basement membranes, J Cell Biol, 117, 1119

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Li, M., Yu, C., Li, Z., Xiong, L., Wang, J., Shang, X., Sun, H., Chang, Y., Du, X., & Zheng, R. (2025) · Discover Oncology · doi:10.1007/s12672-025-04183-7 · Cited by 2
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Immune-reactive tumor organoids system to determine the effects of microbial metabolites on cancer immunity and immunotherapies
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Han, J., Cui, Y., Bai, X., Xue, W., Liu, S., & Dai, J. (2019) · Research for development · doi:10.1007/978-981-13-6325-2_10 · Cited by 3
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Trigub, М. М., Filatova, N. V., Areshidze, D. A., Sen', V. D., & Terentiev, A. A. (2020) · Bulletin of Experimental Biology and Medicine · doi:10.1007/s10517-020-04757-1 · Cited by 2
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Hudson, K., Cross, N. A., Jordan, N., & Leyland, R. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.10.31.514495 · Cited by 2
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The comparison of gold nanoparticles effectiveness in 2D and 3D cell culture.
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Exploring oncology treatment strategies with tyrosine kinase inhibitors through advanced 3D models (Review)
Isinelli, G., Failla, S., Plebani, R., & Prete, A. (2024) · Medicine International · doi:10.3892/mi.2024.212 · Cited by 1
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Luo, Y., Elumalai, A., Humayun, A., & Mills, D. K. (2019) · Springer eBooks · doi:10.1007/978-3-030-23906-0_6 · Cited by 1
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Abstract
(2018) · Translational Sports Medicine · doi:10.1002/tsm2.17
An investigation of the antifungal and antitumor activity of ajoene
Yang, M. (2013) · UC Research Repository (University of Canterbury) · doi:10.26021/6476
Integrated Immunotherapy for Breast Cancer
Lee, P. P. (2013) · Defense Technical Information Center (DTIC) · doi:10.21236/ada591526
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Cortez, B. A. (2014) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.41.2014.tde-15122014-143541
Investigating pathways in tumour cell migration and invasion in response to novel isoquinolinone compounds
Piano, M. D. (2013) · UEA Digital Repository (University of East Anglia)
Avaliação da proliferação e migração celular mediadas pela ativação do EGFR em linhagens celulares de câncer de pulmão cultivadas como monocamadas e esferoides.
Lauand, C. (2015) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.42.2016.tde-25022016-095442 · Cited by 1
Development of a FRET-based 3D in vitro breast tumor model and its application in evaluating drug-induced apoptosis
Anand, P. (2017) · doi:10.32657/10356/70579
Three-Dimensional Cell Culture at the Frontiers of in Vitro Cancer Research: Present Perspectives
Soo, L. L., Zainal, A. A., Hidetsugu, T., Takabatake, K., Nakano, K., Chai, W. L., Nagatsuka, H., & Siar, C. H. (2016) · Annals of Dentistry · doi:10.22452/adum.vol23no1.1
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Rengganaten, V. (2016)
Development of 3D cancer models and targeted fluorescent gold nanoclusters
Dashtarzheneh, A. K. (2018) · UCL Discovery (University College London)
Molecular signalling and interactions in colon cancer stem cells
Chruścik, A. (2019) · Griffith Research Online · doi:10.25904/1912/3787
Prodrugs activated by implantable microsystems
Norman, D. J. (2019) · ERA
Regulation of breast cancer biomarkers by intrinsic and extrinsic factors
Pallegar, N. K. (2018) · Memorial University Research Repository (Memorial University)
Establishment, Characterisation And Mirna Transcriptome Profiling Of Spheroid-Enriched Cells With Breast Cancer Stem Cell Properties
Boo, L. (2020)
Lynch syndrome: current status of CRC in Biotechnological and Biomedical fieldsPreprint
Capuzzo, A. M. (2021) · Preprints.org · doi:10.20944/preprints202107.0303.v1
Investigating the contribution of extracellular matrix to mammary cell behaviour in association with mammographic density
Reye, G. (2021) · Queensland University of Technology · doi:10.5204/thesis.eprints.211468
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Zamora-Pérez, P., Xu, R., Xiao, C., Sobrido, M. S., Rovira-Esteva, M., Conesa, J. J., Jaque, D., & Rivera-Gil, P. (2021) · SSRN Electronic Journal · doi:10.2139/ssrn.3910602

Authors: Luca, A. C., Mersch, S., Deenen, R., Schmidt, S., Messner, I., Schäfer, K.-L., Baldus, S. E., Huckenbeck, W., Piekorz, R. P., Knoefel, W. T., Krieg, A., & Stoecklein, N. H.
Affiliations: Düsseldorf University Hospital (DE)
Heinrich Heine University Düsseldorf (DE)
Corresponding author: Krieg, Andreas
Stoecklein, Nikolas H.
ORCID: Deenen, René
Schmidt, Stephan
Baldus, Stephan E.
Piekorz, Roland P.
Knoefel, Wolfram T.
Krieg, Andreas
Stoecklein, Nikolas H.
Venue: PLoS ONE
Publisher: Public Library of Science (PLoS)
Published: 2013-03-26
Volume: 8
Issue: 3
Pages: e59689
ISSN: 1932-6203 (ISSN-L)
DOI: 10.1371/journal.pone.0059689
PMC: PMC3608563
OpenAlex: W1983051794
Cited by: 341
References: 31
Citations by year: 2026: 11 · 2025: 14 · 2024: 18 · 2023: 29 · 2022: 27 · 2021: 39 · 2020: 40 · 2019: 29 · 2018: 25 · 2017: 33 · 2016: 29 · 2015: 18 · 2014: 25 · 2013: 4
FWCI: 13.56
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (unspecified)
Locations: PLoS ONE (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, other-oa)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Univ. Duesseldorf: Duesseldorfer Dokumenten- und Publikationsserver (submittedVersion)
Figshare (submittedVersion, cc-by)
Funders: Deutsche Forschungsgemeinschaft (SFB728)
Topics: Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords: colorectal cancer, 3D cell culture, tumor microenvironment, EGFR signaling, EGFR inhibition, AG1478, gene expression, cell morphology, spheroid formation, cell invasion, cell migration, KRAS wild-type, 2D vs 3D culture comparison, colorectal cancer cell lines
MeSH terms: Extracellular Matrix, Humans, Laminin, Phenotype, Colorectal Neoplasms, Gene Expression Regulation, Neoplastic, Spheroids, Cellular, Cell Culture Techniques, Cell Line, Tumor, Protein Kinase Inhibitors, Cell Proliferation, Tumor Microenvironment, ErbB Receptors

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Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines

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