The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Phenotypic Characterization of Retinoic Acid Differentiated SH-SY5Y Cells by Transcriptional Profiling
Open accessAbstract
Multiple genetic and environmental factors play a role in the development and progression of Parkinson's disease (PD). The main neuropathological hallmark of PD is the degeneration of dopaminergic (DAergic) neurons in the substantia nigra pars compacta. To study genetic and molecular contributors to the disease process, there is a great need for readily accessible cells with prominent DAergic features that can be used for reproducible in vitro cellular screening. Here, we investigated the molecular phenotype of retinoic acid (RA) differentiated SH-SY5Y cells using genome wide transcriptional profiling combined with gene ontology, transcription factor and molecular pathway analysis. We demonstrated that RA induces a general neuronal differentiation program in SH-SY5Y cells and that these cells develop a predominantly mature DAergic-like neurotransmitter phenotype. This phenotype is characterized by increased dopamine levels together with a substantial suppression of other neurotransmitter phenotypes, such as those for noradrenaline, acetylcholine, glutamate, serotonin and histamine. In addition, we show that RA differentiated SH-SY5Y cells express the dopamine and noradrenalin neurotransmitter transporters that are responsible for uptake of MPP(+), a well known DAergic cell toxicant. MPP(+) treatment alters mitochondrial activity according to its proposed cytotoxic effect in DAergic neurons. Taken together, RA differentiated SH-SY5Y cells have a DAergic-like phenotype, and provide a good cellular screening tool to find novel genes or compounds that affect cytotoxic processes that are associated with PD.
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Cited by
The SH-SY5Y cell line in Parkinson’s disease research: a systematic review
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“DYRK1A primarily induces cell cycle arrest of SH-SY5Y by phosphorylation of cell cycle proteins, which then favors neuronal differentiation, whereas RA induces changes of differentiation-related genes (such as TrkB) in the first place before cells exit the cell cycle.(60,61) Nonetheless, elevated DYRK1A activity event…”
BackgroundHafiz, Z. Z., Amin, M. ‘. M., Johari James, R. M., Teh, L. K., Salleh, M. Z., & Adenan, M. I. (2020) · Molecules · doi:10.3390/molecules25040892 · Cited by 64
Leggio, L., L'Episcopo, F., Magrì, A., Ulloa‐Navas, M. J., Paternò, G., Vivarelli, S., Bastos, C. A. P., Tirolo, C., Testa, N., Caniglia, S., et al. (2022) · Advanced Healthcare Materials · doi:10.1002/adhm.202201203 · Cited by 54
Metadata
- Authors
- Korecka, J. A., van Kesteren, R. E., Blaas, E., Spitzer, S. O., Kamstra, J. H., Smit, A. B., Swaab, D. F., Verhaagen, J., & Bossers, K.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-05-28
- Volume
- 8
- Issue
- 5
- Pages
- e63862
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1966911546
- Cited by
- 253
- References
- 66
- FWCI
- 11.02
- Type
- journal article
- Language
- en
- Open access
- gold
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- Korecka, J. A., van Kesteren, R. E., Blaas, E., Spitzer, S. O., Kamstra, J. H., Smit, A. B., Swaab, D. F., Verhaagen, J., & Bossers, K. (2013). Phenotypic Characterization of Retinoic Acid Differentiated SH-SY5Y Cells by Transcriptional Profiling. PLoS ONE. https://doi.org/10.1371/journal.pone.0063862
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