The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
N-3 Polyunsaturated Fatty Acids (PUFAs) Reverse the Impact of Early-Life Stress on the Gut Microbiota
Open accessAbstract
BACKGROUND: Early life stress is a risk factor for many psychiatric disorders ranging from depression to anxiety. Stress, especially during early life, can induce dysbiosis in the gut microbiota, the key modulators of the bidirectional signalling pathways in the gut-brain axis that underline several neurodevelopmental and psychiatric disorders. Despite their critical role in the development and function of the central nervous system, the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs) on the regulation of gut-microbiota in early-life stress has not been explored. METHODS AND RESULTS: Here, we show that long-term supplementation of eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) (80% EPA, 20% DHA) n-3 PUFAs mixture could restore the disturbed gut-microbiota composition of maternally separated (MS) female rats. Sprague-Dawley female rats were subjected to an early-life stress, maternal separation procedure from postnatal days 2 to 12. Non-separated (NS) and MS rats were administered saline, EPA/DHA 0.4 g/kg/day or EPA/DHA 1 g/kg/day, respectively. Analysis of the gut microbiota in adult rats revealed that EPA/DHA changes composition in the MS, and to a lesser extent the NS rats, and was associated with attenuation of the corticosterone response to acute stress. CONCLUSIONS: In conclusion, EPA/DHA intervention alters the gut microbiota composition of both neurodevelopmentally normal and early-life stressed animals. This study offers insights into the interaction between n-3 PUFAs and gut microbes, which may play an important role in advancing our understanding of disorders of mood and cognitive functioning, such as anxiety and depression.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“While a polyunsaturated fatty acid-rich diet may be exerting its prosocial effects via modulation of fatty acid membrane levels in the brain, it has also been shown to positively affect the composition of the microbiota (Pusceddu et al., 2015;Robertson et al., 2017b) and thus a role for the microbiota is likely (Robertson et al., 2018).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Pusceddu et al., 2015
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Frank, D. N., St. Amand, A. L., Feldman, R. A., Boedeker, E. C., Harpaz, N., & Pace, N. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706625104 · Cited by 4,611
Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour
Cryan, J. F., & Dinan, T. G. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3346 · Cited by 4,572
Self-citationNormal gut microbiota modulates brain development and behavior
Heijtz, R. D., Wang, S., Anuar, F., Qian, Y., Björkholm, B., Samuelsson, A., Hibberd, M. L., Forssberg, H., & Pettersson, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1010529108 · Cited by 3,228
Altered fecal microbiota composition in patients with major depressive disorder
Jiang, H., Ling, Z., Zhang, Y., Mao, H., Ma, Z., Yin, Y., Wang, W., Tang, W., Tan, Z., Shi, J., et al. (2015) · Brain, Behavior, and Immunity · doi:10.1016/j.bbi.2015.03.016 · Cited by 2,275
Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis
Scher, J. U., Sczesnak, A., Longman, R. S., Segata, N., Ubeda, C., Bielski, C., Rostron, T., Cerundolo, V., Pamer, E. G., Abramson, S. B., et al. (2013) · eLife · doi:10.7554/elife.01202 · Cited by 2,047
Bailey, M. T., Dowd, S. E., Galley, J. D., Hufnagle, A. R., Allen, R. G., & Lyte, M. (2011) · Brain, Behavior, and Immunity · doi:10.1016/j.bbi.2010.10.023 · Cited by 1,123
O'Mahony, S. M., Marchesi, J. R., Scully, P., Codling, C., Ceolho, A.-M., Quigley, E. M. M., Cryan, J. F., & Dinan, T. G. (2009) · Biological Psychiatry · doi:10.1016/j.biopsych.2008.06.026 · Cited by 1,069
Self-citationStecher, B., Robbiani, R., Walker, A. W., Westendorf, A. M., Barthel, M., Kremer, M., Chaffron, S., Macpherson, A. J., Buer, J., Parkhill, J., et al. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050244 · Cited by 1,048
Gut microbes and the brain: paradigm shift in neuroscience
Mayer, E. A., Knight, R., Mazmanian, S. K., Cryan, J. F., & Tillisch, K. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.3299-14.2014 · Cited by 1,033
Self-citationPyrosequencing study of fecal microflora of autistic and control children
Finegold, S. M., Dowd, S. E., Gontcharova, V., Liu, C., Henley, K. E., Wolcott, R. D., Youn, E., Summanen, P. H., Granpeesheh, D., Dixon, D., et al. (2010) · Anaerobe · doi:10.1016/j.anaerobe.2010.06.008 · Cited by 977
Flagged first, then by citations
Cited by
Sorted by citations
Items 201–213 of 213
Stress Hormones and the Gut Microbiota Composition and Function
Neuman, H. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819265-8.00070-x
Interplay between gut bacteria and Parkinson’s disease medication
van Kessel, S. P. (2021) · University of Groningen research database (University of Groningen / Centre for Information Technology) · doi:10.33612/diss.182831964
Miller, G. W. (2019) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.734885415.793558390
Fung, V. S. C., & Morales‐Briceño, H. (2019) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.734885415.793558045
Campanari, D. D. (2024) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.17.2024.tde-13052024-155734
Maternal diyetin anne sütü aracılığıyla bebeğin mikrobiyota gelişimine etkisi
Ünal, E. M., & Yılmaz, S. E. (2024) · Sağlık akademisyenleri dergisi/Sağlık akademisyenler dergisi · doi:10.52880/sagakaderg.1347843
Ripple Effects of Early Life Stress on Vascular Health
Kellum, C. E., Kelly, G., & Pollock, J. S. (2025) · Hypertension · doi:10.1161/hypertensionaha.124.17804 · Cited by 1
Karner, T., Forbes, P., Berry, D., & Wagner, I. C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.24.661294
A Potential Link between Gut Leakiness and Sex Differences in Autism Spectrum Disorder (ASD)
Aly, H. F. (2025) · International journal for autism challenges & solution. · doi:10.54878/t52cp193
Modulation de l'apport qualitatif post-natal en lipides sur le fonctionnement cérébral du nouveau-né
Aïdoud, N. (2018) · theses.fr (ABES) · doi:10.70675/6e8c9142za1bcz4dbcza6bfzebbdbfbac84a
Role of the gut-brain axis in early stress-induced emotional vulnerability
Rincel, M. (2017) · theses.fr (ABES) · doi:10.70675/5e8a972cz9ad1z42aezb89cze6474124ef90
Pereira, D. E., Bidô, R. D. C. D. A., Alves, M. D. C., Moura, R. D. L., Costa, A. C. D. S., Dutra, L. M. G., Sá, L. G. D. C. S. E., Viera, V., Ramos, J. F., Freitas, J. C. R., et al. (2026) · Journal of Food Science · doi:10.1111/1750-3841.71191
Kaur, M. K. K., Allahbadia, M. G., & MD, D. M. S. (2025) · Obesity Research - Open Journal · doi:10.17140/oroj.2025.v11.i01.pp19-45.1417
Metadata
- Authors
- Pusceddu, M. M., El Aidy, S., Crispie, F., O’Sullivan, O., Cotter, P., Stanton, C., Kelly, P., Cryan, J. F., & Dinan, T. G.
- Affiliations
- University College Cork (IE)
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- Corresponding author
- Dinan, Timothy G.
- ORCID
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-10-01
- Volume
- 10
- Issue
- 10
- Pages
- e0139721
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4591340
- OpenAlex
- W2152995125
- Cited by
- 214
- References
- 51
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 18 · 2023: 14 · 2022: 17 · 2021: 19 · 2020: 26 · 2019: 30 · 2018: 31 · 2017: 27 · 2016: 19 · 2015: 1
- FWCI
- 25.06
- Type
- journal article
- Language
- en
- Update notices
- correction (2015-10-30)
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion, cc-by)
- Arrow@dit (Dublin Institute of Technology) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- T-Stór (Teagasc) (submittedVersion, cc-by-nc-sa)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion, cc-by)
- Funders
- Pfizer
- GlaxoSmithKline
- Mead Johnson Nutrition
- Food Institutional Research Measure
- Science Foundation Ireland (07/CE/B1368 and 12/RC/2273, 12/RC/2273, 12/RC/, 12/IA/1537, 07/CE/B1368)
- APC Microbiome Institute
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- omega-3 fatty acids, eicosapentaenoic acid, docosahexaenoic acid, early life stress, maternal separation, gut microbiota, dysbiosis, gut-brain axis, anxiety, depression, brain development, dietary supplementation
- MeSH terms
- Gastrointestinal Microbiome, Animals, Behavior, Animal, Female, Male, Maternal Deprivation, Neuropsychological Tests, Stress, Psychological, Fatty Acids, Omega-3, Rats, Sprague-Dawley, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
