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Cited by 23Neuroscience and Neuropharmacology ResearchMicroRNA in disease regulationMemory and Neural Mechanisms

MicroRNAs, miR-23a-3p and miR-151-3p, Are Regulated in Dentate Gyrus Neuropil following Induction of Long-Term Potentiation In Vivo

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Ryan, Brigid, Logan, Barbara J., Abraham, Wickliffe C., Williams, Joanna M.·2017·PLOS ONE
DOI 10.1371/journal.pone.0170407PMC PMC5268419
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Abstract

Translation of synaptic mRNA contributes to alterations in the proteome necessary to consolidate long-term potentiation (LTP), a model of memory processes. Yet, how this process is controlled is not fully resolved. MicroRNAs are non-coding RNAs that negatively regulate gene expression by suppressing translation or promoting mRNA degradation. As specific microRNAs are synaptically located, we hypothesized that they are ideally suited to couple synaptic activation, translational regulation, and LTP persistence. The aim of this study was to identify LTP-regulated microRNAs at or near synapses. Accordingly, LTP was induced unilaterally at perforant path-dentate gyrus synapses in awake adult Sprague-Dawley rats. Five hours later, dentate gyrus middle molecular layer neuropil, containing potentiated synapses, was laser-microdissected. MicroRNA expression profiling, using TaqMan Low Density MicroRNA Microarrays (n = 4), identified eight regulated microRNAs. Subsequent individual TaqMan assays confirmed upregulation of miR-23a-3p (1.30 ± 0.10; p = 0.015) and miR-151-3p (1.17 ± 0.19; p = 0.045) in a second cohort (n = 7). Interestingly, bioinformatic analysis indicated that miR-151-3p and miR-23a-3p regulate synaptic reorganisation and transcription, respectively. In summary, we have demonstrated for the first time that microRNAs are regulated in isolated neuropil following LTP induction in vivo, supporting the hypothesis that synaptic, LTP-responsive microRNAs contribute to LTP persistence via regulation of the synaptic proteome.

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  • Upregulation of miR-23a-3p expression can promote the persistence of LTP by regulating the synaptic proteome.

    “upregulation of miR-23a-3p expression can promote the persistence of LTP by regulating the synaptic proteome [135].”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [135]

    The Abstract reports that miR-23a-3p was upregulated in dentate gyrus neuropil five hours after LTP induction. The Conclusion suggests that miRNA activity may affect changes underlying LTP persistence, but says further work is needed to establish the relationship with LTP maintenance. It does not show that increasing miR-23a-3p promotes persistence by regulating the synaptic proteome. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • miR-23a-3p can be regulated in synapses or nearby nerve fibers induced by memory long-term potentiation in adult SD rats.

    “miR-23a-3p can be regulated in synapses or nearby nerve fibers induced by memory long-term potentiation (LTP) in adult SD rats.”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [135]

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References 48 · Cited by 23

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Authors
Ryan, B., Logan, B. J., Abraham, W. C., & Williams, J. M.
Affiliations
Brain Research New Zealand (NZ)
Corresponding author
Williams, Joanna M.
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2017-01-26
Volume
12
Issue
1
Pages
e0170407
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0170407
PMC
PMC5268419
OpenAlex
W2581829791
Cited by
23
References
48
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 2
FWCI
1.07
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Neurological Foundation of New Zealand (1120-MS)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
microRNA, miR-23a-3p, miR-151-3p, long-term potentiation, dentate gyrus, perforant path, synaptic proteome, memory consolidation, miRNA expression profiling, laser microdissection, transcriptional regulation
MeSH terms
Animals, Gene Expression Regulation, Male, Synapses, Rats, Sprague-Dawley, Long-Term Potentiation, Dentate Gyrus, Neuropil, MicroRNAs, Rats

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