Cited by 41Probiotics and Fermented FoodsInfant Nutrition and HealthGut microbiota and health

Isolation and characterization of five novel probiotic strains from Korean infant and children faeces

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Kook, Sun-Young, Chung, Eui-Chun, Lee, Yaelim, Lee, Dong Wan, Kim, Seokjin·2019·PLOS ONE

Abstract

Probiotics are dietary supplements containing viable, non-pathogenic microorganisms that interact with the gastrointestinal microflora and directly with the immune system. The possible health effects of probiotics include modulating the immune system and exerting antibacterial, anticancer, and anti-mutagenic effects. The purpose of this study was to isolate, identify, and characterize novel strains of probiotics from the faeces of Korean infants. Various assays were conducted to determine the physiological features of candidate probiotic isolates, including Gram staining, 16S rRNA gene sequencing, tolerance assays to stimulated gastric juice and bile salts, adherence ability assays, antibiotic susceptibility testing, and assays of immunomodulatory effects. Based on these morphological and biochemical characteristics, five potential probiotic isolates (Enterococcus faecalis BioE EF71, Lactobacillus fermentum BioE LF11, Lactobacillus plantarum BioE LPL59, Lactobacillus paracasei BioE LP08, and Streptococcus thermophilus BioE ST107) were selected. E. faecalis BioE EF71 and L. plantarum BioE LPL59 showed high tolerance to stimulated gastric juice and bile salts, and S. thermophilus BioE ST107 as well as these two strains exhibited stronger adherence ability than reference strain Lactobacillus rhamnosus GG. All five strains inhibited secretion of lipopolysaccharide-induced pro-inflammatory cytokines IL-6 and TNF-α in RAW264.7 macrophages in vitro. L. fermentum BioE LF11, L. plantarum BioE LPL59, and S. thermophilus BioE ST107 enhanced the production of anti-inflammatory cytokine IL-10. Overall, our findings demonstrate that the five novel strains have potential as safe probiotics and encouraged varying degrees of immunomodulatory effects.

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  • Probiotics prevent colorectal cancer.

    “In addition to modulating the immune system, the positive health effects of taking probiotics include: the improvement of lactose tolerance and digestion [199,205], the prevention and treatment of gastrointestinal infections [206], the prevention of colorectal cancer [203,207], reduction in blood cholesterol levels [208,209,210], and the improvement of mental health via the gut-brain axis [211].”

    From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [203]

    The Introduction (page unavailable) lists anticancer activity and prevention of cancer recurrence as possible effects of probiotics. It does not specifically say probiotics prevent colorectal cancer, and its findings concern immunomodulatory effects in vitro rather than cancer prevention. (LLM, conf=0.86)

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Authors
Kook, S.-Y., Chung, E.-C., Lee, Y., Lee, D. W., & Kim, S.
Corresponding author
Kim, Seokjin
Venue
PLOS ONE
Published
2019-10-31
Volume
14
Issue
10
Pages
e0223913
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2985975915
Cited by
41
References
71
Citations by year
2026: 3 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 6 · 2021: 10 · 2020: 4
FWCI
3.86
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
probiotic strains, infant feces, Enterococcus faecalis, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus paracasei, Streptococcus thermophilus, gastric juice tolerance, bile salt tolerance, bacterial adhesion, antibiotic susceptibility, immunomodulation, anti-inflammatory cytokines, IL-10, interleukin-6, TNF-α, RAW 264.7 macrophages, Korean infants
MeSH terms
RAW 264.7 Cells, Animals, Bacterial Adhesion, Bile Acids and Salts, Child, Child, Preschool, Feces, Female, Humans, Hydrogen-Ion Concentration, Immunologic Factors, Infant, Lipopolysaccharides, Macrophages, Male, Cytokines, Probiotics, Drug Resistance, Bacterial, Mice

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