Cited by 86Probiotics and Fermented FoodsInfant Nutrition and HealthGut microbiota and health

Characterization of bacterial isolates from the microbiota of mothers’ breast milk and their infants

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Kozak, Kimberly, Charbonneau, Duane, Sanozky-Dawes, Rosemary, Klaenhammer, Todd·2015·Gut Microbes

Abstract

This investigation assessed the potential of isolating novel probiotics from mothers and their infants. A subset of 21 isolates among 126 unique bacteria from breast milk and infant stools from 15 mother-infant pairs were examined for simulated GI transit survival, adherence to Caco-2 cells, bacteriocin production, and lack of antibiotic resistance. Of the 21 selected isolates a Lactobacillus crispatus isolate and 3 Lactobacillus gasseri isolates demonstrated good profiles of in vitro GI transit tolerance and Caco-2 cell adherence. Bacteriocin production was observed only by L. gasseri and Enterococcus faecalis isolates. Antibiotic resistance was widespread, although not universal, among isolates from infants. Highly similar isolates (≥ 97% similarity by barcode match) of Bifidobacterium longum subsp. infantis (1 match), Lactobacillus fermentum (2 matches), Lactobacillus gasseri (6 matches), and Enterococcus faecalis (1 match) were isolated from 5 infant-mother pairs. Antibiotic resistance profiles between these isolate matches were similar, except in one case where the L. gasseri isolate from the infant exhibited resistance to erythromycin and tetracycline, not observed in matching mother isolate. In a second case, L. gasseri isolates differed in resistance to ampicillin, chloramphenicol and vancomycin between the mother and infant. In this study, gram positive bacteria isolated from mothers' breast milk as well as their infants exhibited diversity in GI transit survival and acid inhibition of pathogens, but demonstrated limited ability to produce bacteriocins. Mothers and their infants offer the potential for identification of probiotics; however, even in the early stages of development, healthy infants contain isolates with antibiotic resistance.

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Authors
Kozak, K., Charbonneau, D., Sanozky-Dawes, R., & Klaenhammer, T.
Affiliations
  • Procter & Gamble (United States) (US)
  • North Carolina State University (US)
Corresponding author
Charbonneau, Duane
Venue
Gut Microbes
Published
2015-11-02
Volume
6
Issue
6
Pages
341-351
ISSN
  • 1949-0976 (ISSN-L)
  • 1949-0984
OpenAlex
W2231625325
Cited by
86
References
46
Citations by year
2026: 1 · 2025: 4 · 2024: 5 · 2023: 19 · 2022: 10 · 2021: 9 · 2020: 12 · 2019: 12 · 2018: 9 · 2017: 5
FWCI
6.25
Type
journal article
Language
en
Open access
Locations
  • Gut Microbes (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
probiotics, breast milk microbiota, infant gut microbiota, Lactobacillus gasseri, Lactobacillus crispatus, Enterococcus faecalis, Bifidobacterium longum subsp. infantis, Lactobacillus fermentum, antibiotic resistance, bacteriocin production, mother-infant dyads, infant stool
MeSH terms
Bacteria, Bacterial Adhesion, Bacteriocins, Bifidobacterium, Feces, Female, Humans, Infant, Lactobacillus, Microbial Sensitivity Tests, Milk, Human, Mothers, Caco-2 Cells, Probiotics, Gastrointestinal Tract, Microbiota

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