Characterization of bacterial isolates from the microbiota of mothers’ breast milk and their infants
Open accessAbstract
This investigation assessed the potential of isolating novel probiotics from mothers and their infants. A subset of 21 isolates among 126 unique bacteria from breast milk and infant stools from 15 mother-infant pairs were examined for simulated GI transit survival, adherence to Caco-2 cells, bacteriocin production, and lack of antibiotic resistance. Of the 21 selected isolates a Lactobacillus crispatus isolate and 3 Lactobacillus gasseri isolates demonstrated good profiles of in vitro GI transit tolerance and Caco-2 cell adherence. Bacteriocin production was observed only by L. gasseri and Enterococcus faecalis isolates. Antibiotic resistance was widespread, although not universal, among isolates from infants. Highly similar isolates (≥ 97% similarity by barcode match) of Bifidobacterium longum subsp. infantis (1 match), Lactobacillus fermentum (2 matches), Lactobacillus gasseri (6 matches), and Enterococcus faecalis (1 match) were isolated from 5 infant-mother pairs. Antibiotic resistance profiles between these isolate matches were similar, except in one case where the L. gasseri isolate from the infant exhibited resistance to erythromycin and tetracycline, not observed in matching mother isolate. In a second case, L. gasseri isolates differed in resistance to ampicillin, chloramphenicol and vancomycin between the mother and infant. In this study, gram positive bacteria isolated from mothers' breast milk as well as their infants exhibited diversity in GI transit survival and acid inhibition of pathogens, but demonstrated limited ability to produce bacteriocins. Mothers and their infants offer the potential for identification of probiotics; however, even in the early stages of development, healthy infants contain isolates with antibiotic resistance.
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Breastfeeding provides an important source of lactic acid bacteria that enter the infant's body.
“This type of feeding provides an important source of lactic acid bacteria (LAB) that enter the infant's body [20,21].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [21]
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Breast milk, a source of beneficial microbes and associated benefits for infant health
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- Authors
- Kozak, K., Charbonneau, D., Sanozky-Dawes, R., & Klaenhammer, T.
- Affiliations
- Procter & Gamble (United States) (US)
- North Carolina State University (US)
- Corresponding author
- Charbonneau, Duane
- Venue
- Gut Microbes
- Publisher
- Informa UK Limited
- Published
- 2015-11-02
- Volume
- 6
- Issue
- 6
- Pages
- 341-351
- ISSN
- 1949-0976 (ISSN-L)
- 1949-0984
- PMC
- PMC4826109
- OpenAlex
- W2231625325
- Cited by
- 86
- References
- 46
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 19 · 2022: 10 · 2021: 9 · 2020: 12 · 2019: 12 · 2018: 9 · 2017: 5
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut Microbes (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- probiotics, breast milk microbiota, infant gut microbiota, Lactobacillus gasseri, Lactobacillus crispatus, Enterococcus faecalis, Bifidobacterium longum subsp. infantis, Lactobacillus fermentum, antibiotic resistance, bacteriocin production, mother-infant dyads, infant stool
- MeSH terms
- Bacteria, Bacterial Adhesion, Bacteriocins, Bifidobacterium, Feces, Female, Humans, Infant, Lactobacillus, Microbial Sensitivity Tests, Milk, Human, Mothers, Caco-2 Cells, Probiotics, Gastrointestinal Tract, Microbiota
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