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Cited by 183Telomeres, Telomerase, and SenescenceNeuroinflammation and Neurodegeneration MechanismsGenetics, Aging, and Longevity in Model Organisms

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Astrocyte senescence promotes glutamate toxicity in cortical neurons

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Limbad, Chandani, Oron, Tal Ronnen, Alimirah, Fatouma, Davalos, Albert R., Tracy, Tara E., Gan, Li, Desprez, Pierre-Yves, Campisi, Judith·2020·PLOS ONE
DOI 10.1371/journal.pone.0227887PMC PMC6964973
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Abstract

Neurodegeneration is a major age-related pathology. Cognitive decline is characteristic of patients with Alzheimer's and related dementias and cancer patients after chemo- or radio-therapies. A recently emerged driver of these and other age-related pathologies is cellular senescence, a cell fate that entails a permanent cell cycle arrest and pro-inflammatory senescence-associated secretory phenotype (SASP). Although there is a link between inflammation and neurodegenerative diseases, there are many open questions regarding how cellular senescence affects neurodegenerative pathologies. Among the various cell types in the brain, astrocytes are the most abundant. Astrocytes have proliferative capacity and are essential for neuron survival. Here, we investigated the phenotype of primary human astrocytes made senescent by X-irradiation, and identified genes encoding glutamate and potassium transporters as specifically downregulated upon senescence. This down regulation led to neuronal cell death in co-culture assays. Unbiased RNA sequencing of transcripts expressed by non-senescent and senescent astrocytes confirmed that glutamate homeostasis pathway declines upon senescence. Our results suggest a key role for cellular senescence, particularly in astrocytes, in excitotoxicity, which may lead to neurodegeneration including Alzheimer's disease and related dementias.

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Fig 1 Human primary astrocytes undergo senescence and express a SASP upon X-irradiation. (a) Immunofluorescence staining for the astrocyte marker GFAP was performed on primary human astrocytes and IMR-90 fibroblasts. (b) Cellular senescence (SEN) was induced in astrocytes by X-irradiation (IR). Fourteen days after IR, SA-β-gal staining was performed on non-senescent (NS) control cells and SEN cells. Quantification is shown in the right panel. (c-d) Real-time PCR was performed on RNA samples from NS and SEN cells. (c) shows the expression of p16INK4a (left) and LMNB1 (right). (d) shows the expression of three SASP factors, IL-6, IL-8 and CXCL-1. (e) High mobility group box 1 (HMGB1) immunofluorescence staining was performed on NS and SEN astrocytes. Quantification is shown in the right panel (n = 3, where n = experimental replicates). (f-g) ELISAs and AlphaLISAs were performed using conditioned media (CM) collected from NS and SEN cells; (f) shows HMGB1 ELISA results (n = 2, where n = experimental replicates), and (g) shows IL-6 AlphaLISA results (n = 3, where n = experimental replicates). For (b-d) (n = 2), shown are representative results from 3 independent experiments. For (b-g): *p<0.05, **p<0.02, ***p<0.001, ****p<0.0001 (unpaired t test).
Fig 1 Human primary astrocytes undergo senescence and express a SASP upon X-irradiation. (a) Immunofluorescence staining for the astrocyte marker GFAP was performed on primary human astrocytes and IMR-90 fibroblasts. (b) Cellular senescence (SEN) was induced in astrocytes by X-irradiation (IR). Fourteen days after IR, SA-β-gal staining was performed on non-senescent (NS) control cells and SEN cells. Quantification is shown in the right panel. (c-d) Real-time PCR was performed on RNA samples from NS and SEN cells. (c) shows the expression of p16INK4a (left) and LMNB1 (right). (d) shows the expression of three SASP factors, IL-6, IL-8 and CXCL-1. (e) High mobility group box 1 (HMGB1) immunofluorescence staining was performed on NS and SEN astrocytes. Quantification is shown in the right panel (n = 3, where n = experimental replicates). (f-g) ELISAs and AlphaLISAs were performed using conditioned media (CM) collected from NS and SEN cells; (f) shows HMGB1 ELISA results (n = 2, where n = experimental replicates), and (g) shows IL-6 AlphaLISA results (n = 3, where n = experimental replicates). For (b-d) (n = 2), shown are representative results from 3 independent experiments. For (b-g): *p<0.05, **p<0.02, ***p<0.001, ****p<0.0001 (unpaired t test).CC BY · PubMed Central
Fig 2 Senescence in astrocytes downregulates genes that modulate excitotoxicity. (a) Real-time PCR was performed for EAAT1, EAAT2, Kir4.1, and AQP4 mRNA using RNA samples from NS and SEN astrocytes. (b-d) Time course experiments of astrocytes after IR. Samples were collected days 7, 10, 14, 21 after treatment. Real-time PCR was performed on NS and SEN samples at the indicated times; (b) corresponds to EAAT1 gene expression, (c) corresponds to EAAT2 gene expression, and (d) corresponds to Kir4.1 gene expression. (e) Western blotting was performed using protein extracts from NS and SEN 14 days after X-irradiation. Antibodies against EAAT1 and Kir4.1 were used. For (a-e) (n = 2), shown are the representative results from 2 independent experiments. For (a): *p<0.05, **p<0.02, ***p<0.001 (unpaired t test). For (b-d): **p<0.02, ***p<0.001, ****p<0.0001 (ordinary one-way ANOVA).
Fig 2 Senescence in astrocytes downregulates genes that modulate excitotoxicity. (a) Real-time PCR was performed for EAAT1, EAAT2, Kir4.1, and AQP4 mRNA using RNA samples from NS and SEN astrocytes. (b-d) Time course experiments of astrocytes after IR. Samples were collected days 7, 10, 14, 21 after treatment. Real-time PCR was performed on NS and SEN samples at the indicated times; (b) corresponds to EAAT1 gene expression, (c) corresponds to EAAT2 gene expression, and (d) corresponds to Kir4.1 gene expression. (e) Western blotting was performed using protein extracts from NS and SEN 14 days after X-irradiation. Antibodies against EAAT1 and Kir4.1 were used. For (a-e) (n = 2), shown are the representative results from 2 independent experiments. For (a): *p<0.05, **p<0.02, ***p<0.001 (unpaired t test). For (b-d): **p<0.02, ***p<0.001, ****p<0.0001 (ordinary one-way ANOVA).CC BY · PubMed Central
Fig 3 RNA-seq data reveal genes regulated in astrocytes upon senescence induction. (a) RNA extracted from SEN astrocytes corresponding to 6 different human cell strains was compared to RNA extracted from NS samples. p16INK4a and SASP factors (IL-6 and IL-8) were analyzed by real-time PCR. (b) RNA-seq results (presented as Fragments Per Kilobase Million or FPKM) for the expression of various genes, i.e., p16INK4a (left panel), LMNB1 (center panel) and IL-6, IL-8, CXCL-1 (right panel) were compared between NS and SEN samples. (c) Hierarchical clustering using the Jensen-Shannon distance metric was used to determine the similarity within the 6 NS samples and within the 6 SEN samples. (d) A heatmap based on FPKM of Transporter-associated Astrocyte Enriched Genes (TAEG) in all the NS and SEN samples is shown. For (a), n = 2s. For (b), n = 3. For (a, b): *p<0.05, **p<0.02, ***p<0.001, ****p<0.0001 (unpaired t test).
Fig 3 RNA-seq data reveal genes regulated in astrocytes upon senescence induction. (a) RNA extracted from SEN astrocytes corresponding to 6 different human cell strains was compared to RNA extracted from NS samples. p16INK4a and SASP factors (IL-6 and IL-8) were analyzed by real-time PCR. (b) RNA-seq results (presented as Fragments Per Kilobase Million or FPKM) for the expression of various genes, i.e., p16INK4a (left panel), LMNB1 (center panel) and IL-6, IL-8, CXCL-1 (right panel) were compared between NS and SEN samples. (c) Hierarchical clustering using the Jensen-Shannon distance metric was used to determine the similarity within the 6 NS samples and within the 6 SEN samples. (d) A heatmap based on FPKM of Transporter-associated Astrocyte Enriched Genes (TAEG) in all the NS and SEN samples is shown. For (a), n = 2s. For (b), n = 3. For (a, b): *p<0.05, **p<0.02, ***p<0.001, ****p<0.0001 (unpaired t test).CC BY · PubMed Central
Fig 4 Increased neuronal death in co-cultures with SEN astrocytes dependent on glutamate. (a-b) Neurons (only DAPI-stained cells) were co-cultured with NS or SEN astrocytes (CMPTX-red + DAPI-stained cells) in neuronal media. The co-cultures were treated for 24 h with control media without glutamate. In (a) we show the fluorescent images, and in (b) the cell quantification. (c-d) Neurons were also co-cultured with NS or SEN astrocytes in neuronal media. However, here co-cultures were treated for 24 h with media containing 10 mM glutamate. In (c) we show the fluorescent images, and in (d) the cell quantification. Lane 1: surviving neurons (NS astrocytes + neurons co-cultures). Lane 2: surviving neurons (SEN astrocytes + neurons co-cultures). Lane 3: surviving astrocytes (NS astrocytes + neurons co-cultures). Lane 4: surviving astrocytes (SEN astrocytes + neurons co-cultures). For (b, d) (n = 4, where n = experimental replicates): ns = not significant, *p<0.05, **p<0.02 (unpaired t test).
Fig 4 Increased neuronal death in co-cultures with SEN astrocytes dependent on glutamate. (a-b) Neurons (only DAPI-stained cells) were co-cultured with NS or SEN astrocytes (CMPTX-red + DAPI-stained cells) in neuronal media. The co-cultures were treated for 24 h with control media without glutamate. In (a) we show the fluorescent images, and in (b) the cell quantification. (c-d) Neurons were also co-cultured with NS or SEN astrocytes in neuronal media. However, here co-cultures were treated for 24 h with media containing 10 mM glutamate. In (c) we show the fluorescent images, and in (d) the cell quantification. Lane 1: surviving neurons (NS astrocytes + neurons co-cultures). Lane 2: surviving neurons (SEN astrocytes + neurons co-cultures). Lane 3: surviving astrocytes (NS astrocytes + neurons co-cultures). Lane 4: surviving astrocytes (SEN astrocytes + neurons co-cultures). For (b, d) (n = 4, where n = experimental replicates): ns = not significant, *p<0.05, **p<0.02 (unpaired t test).CC BY · PubMed Central

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Metadata

Authors
Limbad, C., Oron, T. R., Alimirah, F., Davalos, A. R., Tracy, T. E., Gan, L., Desprez, P.-Y., & Campisi, J.
Affiliations
  • Buck Institute for Research on Aging (US)
  • Gladstone Institutes (US)
Corresponding author
Campisi, Judith
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2020-01-16
Volume
15
Issue
1
Pages
e0227887
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0227887
PMC
PMC6964973
OpenAlex
W2999084078
Cited by
183
References
63
Citations by year
2026: 18 · 2025: 26 · 2024: 30 · 2023: 26 · 2022: 36 · 2021: 36 · 2020: 11
FWCI
16.39
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
National Institute on Aging (AG009909, AG017242)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Keywords
astrocyte senescence, cellular senescence, glutamate excitotoxicity, excitotoxicity, glutamate transporters, potassium transporters, glutamate homeostasis, neurodegeneration, Alzheimer's disease, senescence-associated secretory phenotype, X-ray irradiation, cortical neurons, neuronal cell death
MeSH terms
Alzheimer Disease, Astrocytes, Brain, Gene Expression Regulation, Humans, Nerve Degeneration, Neurons, X-Rays, Cellular Senescence, Glutamic Acid, Amino Acid Transport System X-AG, Cell Cycle Checkpoints, Primary Cell Culture

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