Inhibition of myostatin prevents microgravity-induced loss of skeletal muscle mass and strength
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The microgravity conditions of prolonged spaceflight are known to result in skeletal muscle atrophy that leads to diminished functional performance. To assess if inhibition of the growth factor myostatin has potential to reverse these effects, mice were treated with a myostatin antibody while housed on the International Space Station. Grip strength of ground control mice increased 3.1% compared to baseline values over the 6 weeks of the study, whereas grip strength measured for the first time in space showed flight animals to be -7.8% decreased in strength compared to baseline values. Control mice in space exhibited, compared to ground-based controls, a smaller increase in DEXA-measured muscle mass (+3.9% vs +5.6% respectively) although the difference was not significant. All individual flight limb muscles analyzed (except for the EDL) weighed significantly less than their ground counterparts at the study end (range -4.4% to -28.4%). Treatment with myostatin antibody YN41 was able to prevent many of these space-induced muscle changes. YN41 was able to block the reduction in muscle grip strength caused by spaceflight and was able to significantly increase the weight of all muscles of flight mice (apart from the EDL). Muscles of YN41-treated flight mice weighed as much as muscles from Ground IgG mice, with the exception of the soleus, demonstrating the ability to prevent spaceflight-induced atrophy. Muscle gene expression analysis demonstrated significant effects of microgravity and myostatin inhibition on many genes. Gamt and Actc1 gene expression was modulated by microgravity and YN41 in opposing directions. Myostatin inhibition did not overcome the significant reduction of microgravity on femoral BMD nor did it increase femoral or vertebral BMD in ground control mice. In summary, myostatin inhibition may be an effective countermeasure to detrimental consequences of skeletal muscle under microgravity conditions.
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A MSTN monoclonal antibody was shown to be capable of increasing muscle mass in mice in microgravity.
“Similar findings were recently reported in which a MSTN monoclonal antibody was shown to be capable of increasing muscle mass in mice in microgravity (25).”
From Targeting myostatin/activin A protects against skeletal muscle and bone loss during spaceflight (2020) · cites this paper as [25]
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Guidelines for assessment of bone microstructure in rodents using micro–computed tomography
Bouxsein, M., Boyd, S. K., Christiansen, B., Guldberg, R. E., Jepsen, K. J., & Müller, R. (2010) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.141 · Cited by 4,461 · see citing works
Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1
Bodine, S. C., & Baehr, L. M. (2014) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00204.2014 · Cited by 1,117 · see citing works
Age-related and disease-related muscle loss: The effect of diabetes, obesity, and other diseases
Kalyani, R. R., Corriere, M., & Ferrucci, L. (2014) · The Lancet Diabetes & Endocrinology · doi:10.1016/s2213-8587(14)70034-8 · Cited by 1,102 · see citing works
Cortical and Trabecular Bone Mineral Loss From the Spine and Hip in Long-Duration Spaceflight
Lang, T., LeBlanc, A., Evans, H., Lu, Y., Genant, H., & Yu, A. (2004) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.040307 · Cited by 828 · see citing works
Age-Related Changes in Trabecular Architecture Differ in Female and Male C57BL/6J Mice
Glatt, V., CANALIS, E. M., Stadmeyer, L., & Bouxsein, M. (2007) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.070507 · Cited by 609 · see citing works
Bone mineral and lean tissue loss after long duration space flight.
LeBlanc, A., Schneider, V. B., Shackelford, L. C., West, S., Oganov, V. S., Bakulin, A. V., & Voronin, L. I. (2000) · PubMed · Cited by 466 · see citing works
Exercise in space: human skeletal muscle after 6 months aboard the International Space Station
Trappe, S., Costill, D., Gallagher, P., Creer, A., Peters, J. R., Evans, H., Riley, D. A., & Fitts, R. H. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91578.2008 · Cited by 454 · see citing works
Myostatin antibody (LY2495655) in older weak fallers: a proof-of-concept, randomised, phase 2 trial
Becker, C., Lord, S. R., Studenski, S. A., Warden, S. J., Fielding, R. A., Recknor, C. P., Hochberg, M., Ferrari, S., Blain, H., Binder, E. F., et al. (2015) · The Lancet Diabetes & Endocrinology · doi:10.1016/s2213-8587(15)00298-3 · Cited by 381 · see citing works
Bone development and age-related bone loss in male C57BL/6J mice
Ferguson, V. L., Ayers, R. A., Bateman, T. A., & Simske, S. J. (2003) · Bone · doi:10.1016/s8756-3282(03)00199-6 · Cited by 379 · see citing works
Self-citationFitts, R. H., Trappe, S. W., COSTILL, D. L., Gallagher, P. M., Creer, A. C., Colloton, P. A., Peters, J., Romatowski, J. G., Bain, J. L. W., & Riley, D. A. (2010) · The Journal of Physiology · doi:10.1113/jphysiol.2010.188508 · Cited by 369 · see citing works
Measuring the Strength of Mice
Deacon, R. M. J. (2013) · Journal of Visualized Experiments · doi:10.3791/2610 · Cited by 298 · see citing works
Allen, D. L., & Unterman, T. G. (2006) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00542.2005 · Cited by 264 · see citing works
Rooks, D., Praestgaard, J., Hariry, S., Laurent, D., Petricoul, O., Perry, R. G., Lach‐Trifilieff, E., & Roubenoff, R. (2017) · Journal of the American Geriatrics Society · doi:10.1111/jgs.14927 · Cited by 244 · see citing works
Legrand, D., Vaes, B., Matheï, C., Adriaensen, W. J., Pottelbergh, G. V., & Degryse, J. (2014) · Journal of the American Geriatrics Society · doi:10.1111/jgs.12840 · Cited by 239 · see citing works
Myostatin (GDF-8) as a key factor linking muscle mass and bone structure.
Elkasrawy, M., & Hamrick, M. W. (2010) · PubMed · Cited by 203 · see citing works
Adaptation of Mouse Skeletal Muscle to Long-Term Microgravity in the MDS Mission
Sandonà, D., Desaphy, J., Camerino, G. M., Bianchini, E., Ciciliot, S., Danieli‐Betto, D., Dobrowolny, G., Furlan, S., Germinario, E., Goto, K., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033232 · Cited by 191 · see citing works
Allen, D. L., Yasui, W., Tanaka, T., Ohira, Y., Nagaoka, S., Sekiguchi, C., Hinds, W. E., Roy, R. R., & Edgerton, V. R. (1996) · Journal of Applied Physiology · doi:10.1152/jappl.1996.81.1.145 · Cited by 190 · see citing works
Lalani, R., Bhasin, S., Byhower, F., Tarnuzzer, R. W., Grant, M. B., Shen, R., Asa, S. L., Ezzat, S., & González-Cadavid, N. F. (2000) · Journal of Endocrinology · doi:10.1677/joe.0.1670417 · Cited by 178 · see citing works
Myostatin inhibitors as therapies for muscle wasting associated with cancer and other disorders
Smith, R. C., & Lin, B. K. (2013) · Current Opinion in Supportive and Palliative Care · doi:10.1097/spc.0000000000000013 · Cited by 151 · see citing works
Self-citationVico, L., van Rietbergen, B., Vilayphiou, N., Linossier, M., Locrelle, H., Normand, M., Zouch, M., Gerbaix, M., Bonnet, N., Novikov, V., et al. (2017) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3188 · Cited by 150 · see citing works
Circulating αKlotho influences phosphate handling by controlling FGF23 production
Smith, R. C., O’Bryan, L. M., Farrow, E., Summers, L. J., Clinkenbeard, E. L., Roberts, J. L., Cass, T. A., Saha, J. K., Broderick, C. L., Ma, Y. L., et al. (2012) · Journal of Clinical Investigation · doi:10.1172/jci64986 · Cited by 149 · see citing works
Self-citationLatres, E., Pangilinan, J., Miloscio, L., Bauerlein, R., Na, E., Potocky, T. B., Huang, Y., Eckersdorff, M., Rafique, A., Mastaitis, J., et al. (2015) · Skeletal Muscle · doi:10.1186/s13395-015-0060-8 · Cited by 143 · see citing works
Age-Related Changes in Bone Structure and Strength in Female and Male BALB/c Mice
Willinghamm, M. D., Brodt, M. D., Lee, K. L., Stephens, A., Ye, J., & Silva, M. J. (2010) · Calcified Tissue International · doi:10.1007/s00223-010-9359-y · Cited by 138 · see citing works
Gerbaix, M., Gnyubkin, V., Farlay, D., Olivier, C., Ammann, P., Courbon, G., Laroche, N., Genthial, R., Follet, H., Peyrin, F., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-03014-2 · Cited by 127 · see citing works
Effects of 17-day spaceflight on knee extensor muscle function and size
Tesch, P. A., Berg, H. E., Bring, D. K. ‐., Evans, H. J., & LeBlanc, A. (2004) · European Journal of Applied Physiology · doi:10.1007/s00421-004-1236-9 · Cited by 120 · see citing works
Chang, D. G., Healey, R., Snyder, A. J., Sayson, J. V., Macias, B. R., Coughlin, D., Bailey, J. F., Parazynski, S. E., Lotz, J. C., & Hargens, A. R. (2016) · Spine · doi:10.1097/brs.0000000000001873 · Cited by 114 · see citing works
Shiba, D., Mizuno, H., Yumoto, A., Shimomura, M., Kobayashi, H., Morita, H., Shimbo, M., Hamada, M., Kudo, T., Shinohara, M., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-10998-4 · Cited by 104 · see citing works
Skeletal muscle adaptations to microgravity exposure in the mouse
Harrison, B. C., Allen, D. L., Girten, B. E., Stodieck, L., Kostenuik, P. J., Bateman, T. A., Morony, S., Lacey, D. L., & Leinwand, L. A. (2003) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00603.2003 · Cited by 99 · see citing works
Self-citationEffect of bimagrumab on thigh muscle volume and composition in men with casting‐induced atrophy
Rooks, D. S., Laurent, D., Praestgaard, J., Rasmussen, S., Bartlett, M., & Tankó, L. B. (2017) · Journal of Cachexia, Sarcopenia and Muscle · doi:10.1002/jcsm.12205 · Cited by 90 · see citing works
Tavella, S., Ruggiu, A., Giuliani, A., Brun, F., Canciani, B., Mănescu, A., Marozzi, K., Cilli, M., Costa, D., Liu, Y., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033179 · Cited by 89 · see citing works
Bimagrumab improves body composition and insulin sensitivity in insulin‐resistant individuals
Garito, T., Roubenoff, R., Hompesch, M., Morrow, L., Gomez, K., Rooks, D., Meyers, C., Buchsbaum, M. S., Neelakantham, S., Swan, T., et al. (2018) · Diabetes, Obesity and Metabolism · doi:10.1111/dom.13042 · Cited by 88 · see citing works
Behavior of mice aboard the International Space Station
Ronca, A. E., Moyer, E. L., Talyansky, Y., Lowe, M. G., Padmanabhan, S., Choi, S. Y., Gong, C.-M. S., Cadena, S. M., Stodieck, L., & Globus, R. K. (2019) · Scientific Reports · doi:10.1038/s41598-019-40789-y · Cited by 80 · see citing works
Self-citationGene Expression Profiling in Slow-Type Calf Soleus Muscle of 30 Days Space-Flown Mice
Gambara, G., Michele Salanova, A., Ciciliot, S., Furlan, S., Gutsmann, M., Schiffl, G., Ungethuem, U., Pompeo Volpe, D., Gunga, H., & Blottner, D. (2017) · PLoS ONE · doi:10.1371/journal.pone.0169314 · Cited by 74 · see citing works
Osteoprotegerin is an effective countermeasure for spaceflight-induced bone loss in mice
Lloyd, S. A., Morony, S. E., Ferguson, V. L., Simske, S. J., Stodieck, L. S., Warmington, K. S., Livingston, E. W., Lacey, D. L., Kostenuik, P. J., & Bateman, T. A. (2015) · Bone · doi:10.1016/j.bone.2015.08.021 · Cited by 68 · see citing works
Self-citationArounleut, P., Bialek, P., Liang, L.-F., Upadhyay, S. K., Fulzele, S., Johnson, M. H., Elsalanty, M. E., Isales, C. M., & Hamrick, M. W. (2013) · Experimental Gerontology · doi:10.1016/j.exger.2013.06.004 · Cited by 66 · see citing works
Kivelä, R., Kyröläinen, H., Selänne, H., Komi, P. V., Kainulainen, H., & Vihko, V. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00531.2007 · Cited by 66 · see citing works
Hanson, A. M., Harrison, B. C., Young, M. H., Stodieck, L., & Ferguson, V. L. (2012) · Muscle & Nerve · doi:10.1002/mus.23753 · Cited by 61 · see citing works
Self-citationTreatment Advances in Spinal Muscular Atrophy
Bharucha-Goebel, D. X., & Kaufmann, P. (2017) · Current Neurology and Neuroscience Reports · doi:10.1007/s11910-017-0798-y · Cited by 43 · see citing works
Berg‐Johansen, B., Liebenberg, E. C., Li, A., Macias, B. R., Hargens, A. R., & Lotz, J. C. (2015) · Journal of Orthopaedic Research® · doi:10.1002/jor.23029 · Cited by 42 · see citing works
Radugina, E. A., Almeida, E. A. C., Blaber, E. A., Poplinskaya, V. A., Markitantova, Y. V., & Grigoryan, E. N. (2017) · Life Sciences in Space Research · doi:10.1016/j.lssr.2017.08.005 · Cited by 40 · see citing works
Smith, R. C., Cramer, M. S., Mitchell, P. J., Capen, A. R., Huber, L., Wang, R., Myers, L., Jones, B. E., Eastwood, B. J., Ballard, D. W., et al. (2015) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-14-0681 · Cited by 39 · see citing works
Self-citationSabsovich, I., Clark, J. D., Liao, G., Peltz, G., Lindsey, D. P., Jacobs, C. R., Yao, W., Guo, T., & Kingery, W. S. (2007) · Bone · doi:10.1016/j.bone.2007.09.041 · Cited by 37 · see citing works
Sung, M., Li, J., Spieker, A. J., Spatz, J., Ellman, R., Ferguson, V. L., Bateman, T. A., Rosen, G. D., Bouxsein, M., & Rutkove, S. B. (2013) · PubMed · Cited by 35 · see citing works
Self-citationKan, H. E., Busé-Pot, T., Navío, R. P., Isbrandt, D., Heerschap, A., & HAAN, A. D. (2005) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00040.2005 · Cited by 34 · see citing works
Masticatory muscles of mouse do not undergo atrophy in space
Philippou, Α., Minozzo, F. C., Spinazzola, J. M., Smith, L., Lei, H., Rassier, D. E., & Barton, E. R. (2015) · The FASEB Journal · doi:10.1096/fj.14-267336 · Cited by 30 · see citing works
Journal of Bone and Mineral Research
Taylor, M. L., Maconnachie, E., Frank, K., BOYDE, A., & Jones, S. J. (1990) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650051391 · Cited by 30 · see citing works
Moyer, E. L., Dumars, P. M., Sun, G.-S., Martin, K. J., Heathcote, D. G., Boyle, R. D., & Skidmore, M. (2016) · npj Microgravity · doi:10.1038/npjmgrav.2016.2 · Cited by 30 · see citing works
Cartilage breakdown in microgravity—a problem for long-term spaceflight?
Fitzgerald, J. (2017) · npj Regenerative Medicine · doi:10.1038/s41536-017-0016-1 · Cited by 30 · see citing works
Sun, G.-S., Tou, J. C. L., Yu, D. S. M., Girten, B. E., & Cohen, J. (2013) · Nutrition · doi:10.1016/j.nut.2013.04.005 · Cited by 29 · see citing works
Prestes, J., Nascimento, D. D. C., Neto, I. V. D. S., Tibana, R. A., Shiguemoto, G. E., Perez, S. E. D. A., Botero, J. P., Schoenfeld, B. J., & Pereira, G. B. (2017) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0000000000001718 · Cited by 28 · see citing works
Effects of spaceflight on the muscles of the murine shoulder
Shen, H., Lim, C., Schwartz, A. G., Andreev-Andrievskiy, A. A., Deymier, A. C., & Thomopoulos, S. (2017) · The FASEB Journal · doi:10.1096/fj.201700320r · Cited by 25 · see citing works
The University of North Carolina at Chapel Hill University Libraries (2020) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/facc-gy93 · Cited by 23 · see citing works
Okragly, A. J., Hamang, M., Pena, E. A., Baker, H. E., Bullock, H. A., Lucchesi, J., Martin, A. P., Ma, Y. L., & Benschop, R. J. (2016) · Cytokine · doi:10.1016/j.cyto.2015.12.011 · Cited by 15 · see citing works
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Update on the effects of microgravity on the musculoskeletal system
Juhl, O. J., Buettmann, E. G., Friedman, M. A., DeNapoli, R. C., Hoppock, G. A., & Donahue, H. J. (2021) · npj Microgravity · doi:10.1038/s41526-021-00158-4 · Cited by 190 · see citing works
Regression of cardiac hypertrophy in health and disease: mechanisms and therapeutic potential
Martin, T. G., Juarros, M. A., & Leinwand, L. A. (2023) · Nature Reviews Cardiology · doi:10.1038/s41569-022-00806-6 · Cited by 180 · see citing works
Targeting myostatin/activin A protects against skeletal muscle and bone loss during spaceflight
Lee, S.-J., Lehar, A., Meir, J. U., Koch, C., Morgan, A., Warren, L. E., Rydzik, R., Youngstrom, D. W., Chandok, H., George, J., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2014716117 · Cited by 115 · see citing works
“Similar findings were recently reported in which a MSTN monoclonal antibody was shown to be capable of increasing muscle mass in mice in microgravity (25).”
Factors mediating spaceflight-induced skeletal muscle atrophy
Lee, P. H. U., Chung, M., Ren, Z., Mair, D. B., & Kim, D. (2022) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00203.2021 · Cited by 104 · see citing works
Principles of the activin receptor signaling pathway and its inhibition
Lodberg, A. (2021) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2021.04.001 · Cited by 62 · see citing works
Biomanufacturing in low Earth orbit for regenerative medicine
Sharma, A., Clemens, R. A., Garcia, O., Taylor, D. L., Wagner, N. L., Shepard, K. A., Gupta, A. E., Malany, S., Grodzinsky, A. J., Kearns‐Jonker, M., et al. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.12.001 · Cited by 54 · see citing works
Antimyostatin Treatment in Health and Disease: The Story of Great Expectations and Limited Success
Nielsen, T. L., Vissing, J., & Krag, T. O. B. (2021) · Cells · doi:10.3390/cells10030533 · Cited by 52 · see citing works
Willey, J. S., Britten, R. A., Blaber, E. A., Tahimic, C. G. T., Chancellor, J., Mortreux, M., Sanford, L. D., Kubik, A. J., Delp, M. D., & Mao, X. W. (2021) · Journal of Environmental Science and Health Part C · doi:10.1080/26896583.2021.1885283 · Cited by 53 · see citing works
Deane, C. S., Willis, C. R. G., Phillips, B. E., Atherton, P. J., Harries, L. W., Ames, R. M., Szewczyk, N. J., & Etheridge, T. (2021) · Journal of Cachexia Sarcopenia and Muscle · doi:10.1002/jcsm.12706 · Cited by 40 · see citing works
Microgravity and Musculoskeletal Health: What Strategies Should Be Used for a Great Challenge?
Bonanni, R., Cariati, I., Marini, M., Tarantino, U., & Tancredi, V. (2023) · Life · doi:10.3390/life13071423 · Cited by 50 · see citing works
To infinity and beyond: Strategies for fabricating medicines in outer space
Seoane‐Viaño, I., Ong, J. J., Basit, A. W., & Goyanes, Á. (2022) · International Journal of Pharmaceutics X · doi:10.1016/j.ijpx.2022.100121 · Cited by 34 · see citing works
Deciphering Myostatin’s Regulatory, Metabolic, and Developmental Influence in Skeletal Diseases
Omosule, C. L., & Phillips, C. L. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.662908 · Cited by 31 · see citing works
Furukawa, S., Chatani, M., Higashitani, A., Higashibata, A., Kawano, F., Nikawa, T., Numaga‐Tomita, T., Ogura, T., Sato, F., Sehara‐Fujisawa, A., et al. (2021) · npj Microgravity · doi:10.1038/s41526-021-00145-9 · Cited by 30 · see citing works
Wang, X., Wei, Z., Gu, M., Zhu, L., Hai, C., Di, A., Wu, D., Bai, C., Su, G., Liu, X., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232415707 · Cited by 29 · see citing works
Lunar gravity prevents skeletal muscle atrophy but not myofiber type shift in mice
Hayashi, T., Fujita, R., Okada, R., Hamada, M., Suzuki, R., Fuseya, S., Leckey, J., Kanai, M., Inoue, Y., Sadaki, S., et al. (2023) · Communications Biology · doi:10.1038/s42003-023-04769-3 · Cited by 29 · see citing works
Cannavò, A., Carandina, A., Corbi, G., Tobaldini, E., Montano, N., & Arosio, B. (2022) · Life · doi:10.3390/life12122139 · Cited by 28 · see citing works
Advanced material technologies for space and terrestrial medicine
Chua, C. Y. X., Jiménez, M., Mozneb, M., Traverso, G., Lugo, R., Sharma, A., Svendsen, C. N., Wagner, W. R., Langer, R., & Grattoni, A. (2024) · Nature Reviews Materials · doi:10.1038/s41578-024-00691-0 · Cited by 31 · see citing works
Mechanisms and Countermeasures for Muscle Atrophy in Microgravity
Liu, Y., Cao, X., Zhou, Q., Deng, C., Yang, Y., Huang, D., Luo, H., Zhang, S., Li, Y., Xu, J., et al. (2024) · Cells · doi:10.3390/cells13242120 · Cited by 23 · see citing works
Genchi, G. G., Degl’Innocenti, A., Martinelli, C., Battaglini, M., Pasquale, D. D., Prato, M., Marras, S., Pugliese, G., Drago, F., Mariani, A., et al. (2021) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.1c14176 · Cited by 22 · see citing works
Manian, V. B., Orozco-Sandoval, J., & Díaz-Martínez, V. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.732370 · Cited by 18 · see citing works
Omosule, C. L., Joseph, D., Weiler, B., Gremminger, V. L., Silvey, S., Jeong, Y., Rafique, A., Krueger, P., Kleiner, S., & Phillips, C. L. (2020) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.4529 · Cited by 18 · see citing works
Ren, Z., Harriot, A. D., Mair, D. B., Chung, M. K., Lee, P. H. U., & Kim, D. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300157 · Cited by 18 · see citing works
Manna, O. M., Burgio, S., Picone, D., Carista, A., Pitruzzella, A., Fucarino, A., & Bucchieri, F. (2024) · Biology · doi:10.3390/biology13110921 · Cited by 18 · see citing works
Hanson, A. M., Young, M. H., Harrison, B. C., Zhou, X., Han, H. Q., Stodieck, L., & Ferguson, V. L. (2023) · npj Microgravity · doi:10.1038/s41526-022-00233-4 · Cited by 15 · see citing works
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Andreev-Andrievskiy, A. A., Dolgov, O., Alberts, J. R., Popova, A., Lagereva, E., Anokhin, K. V., & Виноградова, О. Л. (2021) · Behavioural Brain Research · doi:10.1016/j.bbr.2021.113682 · Cited by 13 · see citing works
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Minoretti, P., Fontana, G. A., Lavdas, K., & D’Acquino, D. (2024) · Cureus · doi:10.7759/cureus.57173 · Cited by 8 · see citing works
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Schulz, H., Strauch, S. M., Richter, P. R., Wehland, M., Krüger, M., Sahana, J., Corydon, T. J., Wise, P. M., Baran, R., Lebert, M., et al. (2022) · Expert Review of Proteomics · doi:10.1080/14789450.2022.2030711 · Cited by 7 · see citing works
Omosule, C. L., Joseph, D., Weiler, B., Gremminger, V. L., Silvey, S., Lafaver, B. N., Jeong, Y., Kleiner, S., & Phillips, C. L. (2023) · JBMR Plus · doi:10.1002/jbm4.10753 · Cited by 7 · see citing works
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Fournier, R., & Harrison, R. E. (2020) · REACH · doi:10.1016/j.reach.2020.100036 · Cited by 7 · see citing works
Opportunities for Biomanufacturing in Low Earth Orbit: Current Status and Future DirectionsPreprint
Giulianotti, M. A., Sharma, A., Clemens, R. A., Garcia, O., Taylor, L., Wagner, N. L., Shepard, K. A., Gupta, A., Malany, S., Grodzinsky, A. J., et al. (2021) · Preprints.org · doi:10.20944/preprints202108.0044.v1 · Cited by 6 · see citing works
Musculoskeletal perturbations of deep space radiation: Assessment using a Gateway MRI
Jullienne, A., Malo, M. E., Shaw, K. A., Zheng, Y., Johnston, J. D., Kontulainen, S. A., Chilibeck, P. D., Dadachova, E., Obenaus, A., & Sarty, G. E. (2024) · Life Sciences in Space Research · doi:10.1016/j.lssr.2024.05.004 · Cited by 6 · see citing works
Long duration space missions: Challenges and prospects in sustaining humans in space
Kapoor, P., Yadav, R., Agrawal, N., Gaur, S., & Arora, R. (2025) · Life Sciences in Space Research · doi:10.1016/j.lssr.2025.05.001 · Cited by 5 · see citing works
Impact of Spaceflight on Upper Extremity Orthopedic Health
Fiedler, B., Jami, M., Rakauskas, T. R., & Ahmed, A. S. (2024) · Aerospace Medicine and Human Performance · doi:10.3357/amhp.6482.2024 · Cited by 4 · see citing works
GDF8 Contributes to Liver Fibrogenesis and Concomitant Skeletal Muscle Wasting
Culver, A. E., Hamang, M., Wang, Y., Jiang, H., Yanum, J. A., White, E., Gawrieh, S., Vuppalanchi, R., Chalasani, N., Dai, G., et al. (2023) · Biomedicines · doi:10.3390/biomedicines11071909 · Cited by 5 · see citing works
Effects of spaceflight on cancellous and cortical bone in proximal femur in growing rats
Gamboa, A., Branscum, A. J., Olson, D. A., Sattgast, L. H., Iwaniec, U. T., & Turner, R. T. (2021) · Bone Reports · doi:10.1016/j.bonr.2021.100755 · Cited by 4 · see citing works
Caicedo, A., Castañeda, V., Díaz, J., Haro-Vinueza, A., Park, J., Kim, J. K., Overbey, E. G., Salinas, I., Nardocci, G., Camera, A., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-2819258/v1 · Cited by 3 · see citing works
Friedman, M. A., Zeineddine, Y., Tuyambaze, O., Elhawabri, W., Shammary, A. A., Stodieck, L., Ferguson, V. L., & Donahue, H. J. (2025) · Bone Reports · doi:10.1016/j.bonr.2025.101871 · Cited by 2 · see citing works
Self-citationChen, M., Gao, X., Chen, J., Xu, F., & Li, Y. (2025) · Space habitation. · doi:10.1016/j.spaceh.2025.100018 · Cited by 2 · see citing works
From aging to space: A comparative biology of skeletal muscle degeneration
Qaisar, R. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2025.168058 · Cited by 2 · see citing works
Bhuyan, N., Ghose, S., Bhattacharya, S., & Chakraborty, T. (2023) · Sciences of Phytochemistry · doi:10.58920/sciphy02010082 · Cited by 1 · see citing works
Rahmanian, N., Hekmat, A., & Hajebrahimi, Z. (2022) · علوم و فناوری فضایی · doi:10.30699/jsst.2021.1346
Tsuji, R., Fujita, R., Hayashi, T., Sadaki, S., Matsumoto, T., Inoue, Y., Murakami, Y., Hamada, M., Muratani, M., Kobayashi, H., et al. (2026) · Science Advances · doi:10.1126/sciadv.aed2258
Analysis of performance decay in harsh space environment of perovskite solar cell
Surendra, G. G., Prasanna, J. L., Kumar, M. R., & Kumar, A. (2026) · Discover Electronics · doi:10.1007/s44291-026-00194-y
The Data Science of Health in Space
Willett, J., Kim, J., Sakharkar, A., Park, J., Agyemang, A. A., Saygili, E. A., Proszynski, J., Nelson, T. M., & Mason, C. E. (2026) · Annual Review of Biomedical Data Science · doi:10.1146/annurev-biodatasci-092724-045605
Putting the brakes on muscle growth: Myostatin as regulator of disuse atrophy
Nieuwenhuijsen, D. H. C., Eggelbusch, M., & Wüst, R. C. I. (2026) · Experimental Physiology · doi:10.1113/ep093732
Quantitative MRI changes in neck muscle size and composition following long‐duration spaceflight
Madrid, D. A., Sawires, M., Lenchik, L., Tooze, J. A., & Weaver, A. A. (2026) · Experimental Physiology · doi:10.1113/ep093726
- Authors
- Smith, R. C., Cramer, M. S., Mitchell, P. J., Lucchesi, J., Ortega, A. M., Livingston, E. W., Ballard, D., Zhang, L., Hanson, J., Barton, K., Berens, S., Credille, K. M., Bateman, T. A., Ferguson, V. L., Ma, Y. L., & Stodieck, L. S.
- Affiliations
- Eli Lilly (United States) (US)
- University of Colorado Boulder (US)
- University of North Carolina at Chapel Hill (US)
- Techshot (United States) (US)
- Corresponding author
- Smith, Rosamund C.
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2020-04-21
- Volume
- 15
- Issue
- 4
- Pages
- e0230818
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC7173869
- OpenAlex
- W3017383204
- Cited by
- 48
- References
- 53
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 14 · 2020: 3
- FWCI
- 4.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- CU Scholar (University of Colorado Boulder) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Center for the Advancement of Science in Space (GA-2016-239, T/)#003, GA-2016-239, T/O#003, GA-2016-239,T/O#003)
- National Aeronautics and Space Administration (NNJ10GA25A)
- Topics
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Space exploration and regulation
- Keywords
- myostatin inhibition, microgravity, spaceflight, skeletal muscle atrophy, muscle mass, muscle strength, grip strength, muscle gene expression, GAMT, ACTC1, bone mineral density, countermeasures, International Space Station
- MeSH terms
- Actins, Animals, Extremities, Femur, Immunoglobulin G, Mice, Inbred BALB C, Muscular Atrophy, Space Flight, Weightlessness, Gene Expression, Muscle, Skeletal, Guanidinoacetate N-Methyltransferase, Mice, Muscle Strength, Myostatin
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