The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
Open accessAbstract
Extracellular vesicles (EVs) in human blood are a potential source of biomarkers. To which extent anticoagulation affects their concentration, cellular origin and protein composition is largely unexplored. To study this, blood from 23 healthy subjects was collected in acid citrate dextrose (ACD), citrate or EDTA, or without anticoagulation to obtain serum. EVs were isolated by ultracentrifugation or by size-exclusion chromatography (SEC) for fluorescence-SEC. EVs were analyzed by micro flow cytometry, NTA, TEM, Western blot, and protein mass spectrometry. The plasma EV concentration was unaffected by anticoagulants, but serum contained more platelet EVs. The protein composition of plasma EVs differed between anticoagulants, and between plasma and serum. Comparison to other studies further revealed that the shared EV protein composition resembles the "protein corona" of synthetic nanoparticles incubated in plasma or serum. In conclusion, we have validated a higher concentration of platelet EVs in serum than plasma by contemporary EV methods. Anticoagulation should be carefully described (i) to enable study comparison, (ii) to utilize available sample cohorts, and (iii) when preparing/selecting biobank samples. Further, the similarity of the EV protein corona and that of nanoparticles implicates that EVs carry both intravesicular and extravesicular cargo, which will expand their applicability for biomarker discovery.
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Cites
Nanoparticle Size and Surface Chemistry Determine Serum Protein Adsorption and Macrophage Uptake
Walkey, C. D., Olsen, J. B., Guo, H., Emili, A., & Chan, W. C. W. (2012) · Journal of the American Chemical Society · doi:10.1021/ja2084338 · Cited by 1,926
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
EV-TRACK Consortium, Van Deun, J., Mestdagh, P., Agostinis, P., Akay, Ö., Anand, S., Anckaert, J., Martinez, Z. A., Baetens, T., Beghein, E., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4185 · Cited by 1,284
Self-citationComplement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo
Chen, F., Wang, G., Griffin, J. I., Brenneman, B., Banda, N. K., Holers, V. M., Backos, D. S., Wu, L., Moghimi, S. M., & Simberg, D. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2016.269 · Cited by 599
Physical exercise induces rapid release of small extracellular vesicles into the circulation
Frühbeis, C., Helmig, S., Tug, S., Simon, P., & Krämer‐Albers, E. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.28239 · Cited by 360
Cited by
Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., et al. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12404 · Cited by 4,663
Self-citationFormation of a protein corona on the surface of extracellular vesicles in blood plasma
Tóth, E. Á., Turiák, L., Visnovitz, T., Cserép, C., Mázló, A., Sódar, B. W., Försönits, A. I., Petővári, G., Sebestyén, A., Komlósi, Z., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12140 · Cited by 522
Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón‐Pérez, J. M., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12093 · Cited by 404
Self-citationExtracellular Vesicles as Novel Diagnostic and Prognostic Biomarkers for Parkinson’s Disease
Leggio, L., Paternò, G., Vivarelli, S., Falzone, G. G., Giachino, C., Marchetti, B., & Iraci, N. (2021) · Aging and disease · doi:10.14336/ad.2021.0527 · Cited by 55
Ananbeh, H., Novak, J., Juhas, S., Juhasova, J., Klempir, J., Doleckova, K., Rysankova, I., Turnovcova, K., Hanus, J., Hansikova, H., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23105598 · Cited by 46
Metadata
- Authors
- Palviainen, M., Saraswat, M., Varga, Z., Kitka, D., Neuvonen, M., Puhka, M., Joenväärä, S., Renkonen, R., Nieuwland, R., Takatalo, M., & Siljander, P. R. M.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2020-08-19
- Volume
- 15
- Issue
- 8
- Pages
- e0236439
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W3072660569
- Cited by
- 287
- References
- 4
- FWCI
- 10.58
- Type
- journal article
- Language
- en
- Open access
- gold
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- CoChat. (2026). Citation check: Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery. https://app.cochat.ai/papers/10.1371/journal.pone.0236439
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- Palviainen, M., Saraswat, M., Varga, Z., Kitka, D., Neuvonen, M., Puhka, M., Joenväärä, S., Renkonen, R., Nieuwland, R., Takatalo, M., & Siljander, P. R. M. (2020). Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery. PLOS ONE. https://doi.org/10.1371/journal.pone.0236439
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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