Genetic impacts on thermostability of onco-lncRNA HOTAIR during the development and progression of endometriosis
Open accessAbstract
HOTAIR is a well-known long non-coding RNA (lncRNA) involved in various cellular signaling, whereas its functional impacts on endometriosis development are still largely unknown. To this end, six potential functional single nucleotide polymorphisms (SNPs) in HOTAIR, with minor allele frequencies more than 10% in Han population and altered net energy of RNA structures larger than 0.5 kcal/mol, were selected for genotyping study. The study included 207 endometriosis patients and 200 healthy women. Genetic substitutions at rs1838169 and rs17720428 were frequently found in endometriosis patients, and rs1838169 showed statistical significance (p= 0.0174). The G-G (rs1838169-rs17720428) haplotype showed the most significant association with endometriosis (p< 0.0001) with enhanced HOTAIR stability, and patients who harbor such haplotype tended to show higher CA125. Data mining further revealed higher mRNA HOTAIR levels in the endometria of patients with severe endometriosis which consistently showed reduced HOXD10 and HOXA5 levels. HOTAIR knockdown with specific shRNAs down-regulated cell proliferation and migration with the induction of HOXD10 and HOXA5 expression in human ovarian clear cancer cells. Our study therefore provided evidence to indicate a prominent role of HOTAIR in promoting endometriosis, which could be used as a potential target for clinical applications.
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Research indicates a significant role for HOTAIR in promoting endometriosis.
“Research indicates a significant role for HOTAIR in promoting endometriosis [175].”
From Endometriosis: Epidemiology, Classification, Pathogenesis, Treatment and Genetics (Review of Literature) (2021) · cites this paper as [175]
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- Authors
- Chang, C. Y.-Y., Tseng, C.-C., Lai, M.-T., Chiang, A.-J., Lo, L.-C., Chen, C.-M., Yen, M.-J., Sun, L., Yang, L., Hwang, T., Tsai, F.-J., & Sheu, J. J.-C.
- Affiliations
- China Medical University (TW)
- National Sun Yat-sen University (TW)
- Ministry of Health and Welfare (TW)
- China Medical University Hospital (TW)
- Qingdao University (CN)
- Third Affiliated Hospital of Zhengzhou University (CN)
- Corresponding author
- Tsai, Fuu-Jen
- Sheu, Jim Jinn-Chyuan
- ORCID
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2021-03-05
- Volume
- 16
- Issue
- 3
- Pages
- e0248168
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC7935326
- OpenAlex
- W3135846059
- Cited by
- 20
- References
- 48
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 4
- FWCI
- 3.67
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Science and Technology, Taiwan ((MOST 106-2314-B-110-001-MY3)
- China Medical University Hospital, Taiwan (DMR107-076)
- National Sun Yat-sen University-Kaohsiung Medical University (108-P013)
- National Sun Yat-sen University (VGHNSYSU108-010)
- National Sun Yat-sen University-Kaohsiung Veterans General Hospital (VGHKS108-122)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- HOTAIR, long non-coding RNA, endometriosis, single nucleotide polymorphisms, genetic susceptibility, haplotype association, CA125, HOXD10, HOXA5, ovarian clear cell carcinoma, cell proliferation and migration, endometrium
- MeSH terms
- Endometriosis, Female, Gene Expression Regulation, Humans, Transcription Factors, Homeodomain Proteins, Polymorphism, Single Nucleotide, RNA Stability, Cell Line, Tumor, RNA, Long Noncoding
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