The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
H-NS Mediates the Silencing of Laterally Acquired Genes in Bacteria
Open accessAbstract
Histone-like nucleoid structuring protein (H-NS) is a modular protein that is associated with the bacterial nucleoid. We used chromatin immunoprecipitation to determine the binding sites of H-NS and RNA polymerase on the Salmonella enterica serovar Typhimurium chromosome. We found that H-NS does not bind to actively transcribed genes and does not co-localize with RNA polymerase. This shows that H-NS principally silences gene expression by restricting the access of RNA polymerase to the DNA. H-NS had previously been shown to preferentially bind to curved DNA in vitro. In fact, at the genomic level we discovered that the level of H-NS binding correlates better with the AT-content of DNA. This is likely to have evolutionary consequences because we show that H-NS binds to many Salmonella genes acquired by lateral gene transfer, and functions as a gene silencer. The removal of H-NS from the cell causes un-controlled expression of several Salmonella pathogenicity islands, and we demonstrate that this has deleterious consequences for bacterial fitness. Our discovery of this novel role for H-NS may have implications for the acquisition of foreign genes by enteric bacteria.
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Cites
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Selective Silencing of Foreign DNA with Low GC Content by the H-NS Protein in Salmonella
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Self-citation
Cited by
Rolfe, M. D., Rice, C. J., Lucchini, S., Pin, C., Thompson, A., Cameron, A. D. S., Alston, M., Stringer, M. F., Betts, R. P., Baranyi, J., et al. (2012) · Journal of Bacteriology · doi:10.1128/jb.06112-11 · Cited by 711
Self-citationThe Ecology and Evolution of Pangenomes
Brockhurst, M. A., Harrison, E., Hall, J. P. J., Richards, T., McNally, A., & MacLean, C. (2019) · Current Biology · doi:10.1016/j.cub.2019.08.012 · Cited by 385
Salmonella Pathogenicity Island 1 (SPI-1) and Its Complex Regulatory Network
Lou, L., Zhang, P., Piao, R., & Wang, Y. (2019) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2019.00270 · Cited by 373
The Accessory Genome of Pseudomonas aeruginosa
Kung, V. L., Ozer, E. A., & Hauser, A. R. (2010) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00027-10 · Cited by 334
Bacterial Stress Responses during Host Infection
Fang, F. C., Frawley, E. R., Tapscott, T., & Vázquez-Torres, A. (2016) · Cell Host & Microbe · doi:10.1016/j.chom.2016.07.009 · Cited by 319
Metadata
- Authors
- Lucchini, S., Rowley, G., Goldberg, M. D., Hurd, D., Harrison, M., & Hinton, J. C. D.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2006-08-18
- Volume
- 2
- Issue
- 8
- Pages
- e81
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- OpenAlex
- W1985963323
- Cited by
- 554
- References
- 30
- FWCI
- 18.38
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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