The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Rapid Quantification of the Latent Reservoir for HIV-1 Using a Viral Outgrowth Assay
Open accessAbstract
HIV-1 persists in infected individuals in a stable pool of resting CD4(+) T cells as a latent but replication-competent provirus. This latent reservoir is the major barrier to the eradication of HIV-1. Clinical trials are currently underway investigating the effects of latency-disrupting compounds on the persistence of the latent reservoir in infected individuals. To accurately assess the effects of such compounds, accurate assays to measure the frequency of latently infected cells are essential. The development of a simpler assay for the latent reservoir has been identified as a major AIDS research priority. We report here the development and validation of a rapid viral outgrowth assay that quantifies the frequency of cells that can release replication-competent virus following cellular activation. This new assay utilizes bead and column-based purification of resting CD4(+) T cells from the peripheral blood of HIV-1 infected patients rather than cell sorting to obtain comparable resting CD4(+) T cell purity. This new assay also utilizes the MOLT-4/CCR5 cell line for viral expansion, producing statistically comparable measurements of the frequency of latent HIV-1 infection. Finally, this new assay employs a novel quantitative RT-PCR specific for polyadenylated HIV-1 RNA for virus detection, which we demonstrate is a more sensitive and cost-effective method to detect HIV-1 replication than expensive commercial ELISA detection methods. The reductions in both labor and cost make this assay suitable for quantifying the frequency of latently infected cells in clinical trials of HIV-1 eradication strategies.
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Citations
Cites
Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy
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Self-citationRecovery of replication-competent HIV despite prolonged suppression of plasma viremia
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Self-citationQuantification of latent tissue reservoirs and total body viral load in HIV-1 infection
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Self-citationSiliciano, J. D., Kajdas, J., Finzi, D., Quinn, T. C., Chadwick, K., Margolick, J. B., Kovacs, C., Gange, S. J., & Siliciano, R. F. (2003) · Nature Medicine · doi:10.1038/nm880 · Cited by 1,703
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Cited by
Replication-competent noninduced proviruses in the latent reservoir increase barrier to HIV-1 cure
Ho, Y.-C., Shan, L., Hosmane, N. N., Wang, J., Laskey, S. B., Rosenbloom, D. I. S., Lai, J., Blankson, J. N., Siliciano, J. D., & Siliciano, R. F. (2013) · Cell · doi:10.1016/j.cell.2013.09.020 · Cited by 1,405
Self-citationDefective proviruses rapidly accumulate during acute HIV-1 infection
Bruner, K. M., Murray, A. J., Pollack, R. A., Soliman, M. G., Laskey, S. B., Capoferri, A. A., Lai, J., Strain, M. C., Lada, S. M., Hoh, R., et al. (2016) · Nature Medicine · doi:10.1038/nm.4156 · Cited by 737
Self-citationA quantitative approach for measuring the reservoir of latent HIV-1 proviruses
Bruner, K. M., Wang, Z., Simonetti, F. R., Bender, A. M., Kwon, K. J., Sengupta, S., Fray, E. J., Beg, S. A., Antar, A. A. R., Jenike, K. M., et al. (2019) · Nature · doi:10.1038/s41586-019-0898-8 · Cited by 718
Self-citationHIV-1 Integration Landscape during Latent and Active Infection
Cohn, L. B., Silva, I. T., Oliveira, T. Y., Rosales, R. A., Parrish, E. H., Learn, G. H., Hahn, B. H., Czartoski, J. L., McElrath, M. J., Lehmann, C., et al. (2015) · Cell · doi:10.1016/j.cell.2015.01.020 · Cited by 444
Self-citationInternational AIDS Society global scientific strategy: towards an HIV cure 2016
International AIDS Society Towards a Cure Working Group, Deeks, S. G., Lewin, S. R., Ross, A. L., Ananworanich, J., Benkirane, M., Cannon, P., Chomont, N., Douek, D., Lifson, J. D., et al. (2016) · Nature Medicine · doi:10.1038/nm.4108 · Cited by 425
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Metadata
- Authors
- Laird, G. M., Eisele, E. E., Rabi, S. A., Lai, J., Chioma, S., Blankson, J. N., Siliciano, J. D., & Siliciano, R. F.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-05-30
- Volume
- 9
- Issue
- 5
- Pages
- e1003398
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- OpenAlex
- W2157096116
- Cited by
- 239
- References
- 5
- FWCI
- 8.59
- Type
- journal article
- Language
- en
- Open access
- gold
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- Laird, G. M., Eisele, E. E., Rabi, S. A., Lai, J., Chioma, S., Blankson, J. N., Siliciano, J. D., & Siliciano, R. F. (2013). Rapid Quantification of the Latent Reservoir for HIV-1 Using a Viral Outgrowth Assay. PLoS Pathogens. https://doi.org/10.1371/journal.ppat.1003398
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