Control of β-glucan exposure by the endo-1,3-glucanase Eng1 in Candida albicans modulates virulence
Open accessPublished version of a preprint
Abstract
Candida albicans is a major opportunistic pathogen of humans. It can grow as morphologically distinct yeast, pseudohyphae and hyphae, and the ability to switch reversibly among different forms is critical for its virulence. The relationship between morphogenesis and innate immune recognition is not quite clear. Dectin-1 is a major C-type lectin receptor that recognizes β-glucan in the fungal cell wall. C . albicans β-glucan is usually masked by the outer mannan layer of the cell wall. Whether and how β-glucan masking is differentially regulated during hyphal morphogenesis is not fully understood. Here we show that the endo-1,3-glucanase Eng1 is differentially expressed in yeast, and together with Yeast Wall Protein 1 (Ywp1), regulates β-glucan exposure and Dectin-1-dependent immune activation of macrophage by yeast cells. ENG1 deletion results in enhanced Dectin-1 binding at the septa of yeast cells; while eng1 ywp1 yeast cells show strong overall Dectin-1 binding similar to hyphae of wild-type and eng1 mutants. Correlatively, hyphae of wild-type and eng1 induced similar levels of cytokines in macrophage. ENG1 expression and Eng1-mediated β-glucan trimming are also regulated by antifungal drugs, lactate and N-acetylglucosamine. Deletion of ENG1 modulates virulence in the mouse model of hematogenously disseminated candidiasis in a Dectin-1-dependent manner. The eng1 mutant exhibited attenuated lethality in male mice, but enhanced lethality in female mice, which was associated with a stronger renal immune response and lower fungal burden. Thus, Eng1-regulated β-glucan exposure in yeast cells modulates the balance between immune protection and immunopathogenesis during disseminated candidiasis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Among the regulated cell wall genes were homologues of C. albicans glucanase genes (XOG1 and ENG1) as well as genes encoding putative adhesins (ALS4, IFF4, and PGA6).
“Among the regulated cell wall genes were homologues of C. albicans glucanase genes (XOG1 and ENG1) (46, 47) as well as genes encoding putative adhesins (ALS4, IFF4, and PGA6) (48–50).”
From Transcriptomics and Phenotyping Define Genetic Signatures Associated with Echinocandin Resistance in Candida auris (2022) · cites this paper as [47]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Double-slit photoelectron interference in strong-field ionization of the neon dimer
Kunitski, M., Eicke, N., Huber, P., Köhler, J., Zeller, S., Voigtsberger, J., Schlott, N., Henrichs, K., Sann, H., Trinter, F., et al. (2019) · Nature Communications · doi:10.1038/s41467-018-07882-8 · Cited by 8,162 · see citing works
Hidden Killers: Human Fungal Infections
Brown, G. D., Denning, D. W., Gow, N. A. R., Levitz, S. M., Netea, M. G., & White, T. C. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004404 · Cited by 4,284 · see citing works
SMARCAD1 ATPase activity is required to silence endogenous retroviruses in embryonic stem cells
Sachs, P., Ding, D., Bergmaier, P., Lamp, B., Schlagheck, C., Finkernagel, F., Nist, A., Stiewe, T., & Mermoud, J. E. (2019) · Nature Communications · doi:10.1038/s41467-019-09078-0 · Cited by 2,388 · see citing works
Nonfilamentous C. albicans Mutants Are Avirulent
Lo, H.-J., Köhler, J. R., DiDomenico, B., Loebenberg, D., Cacciapuoti, A., & Fink, G. R. (1997) · Cell · doi:10.1016/s0092-8674(00)80358-x · Cited by 1,809 · see citing works
Dectin-1: a signalling non-TLR pattern-recognition receptor
Brown, G. D. (2006) · Nature Reviews Immunology · doi:10.1038/nri1745 · Cited by 1,158 · see citing works
Dectin-1 is required for β-glucan recognition and control of fungal infection
Taylor, P. R., Tsoni, S. V., Willment, J. A., Dennehy, K. M., Rosas, M., Findon, H., Haynes, K., Steele, C. H., Botto, M., Gordon, S., et al. (2006) · Nature Immunology · doi:10.1038/ni1408 · Cited by 1,155 · see citing works
Candidalysin is a fungal peptide toxin critical for mucosal infection
Moyes, D. L., Wilson, D., Richardson, J. P., Mogavero, S., Tang, S. X., Wernecke, J., Hoefs, S., Gratacap, R. L., Robbins, J., Runglall, M., et al. (2016) · Nature · doi:10.1038/nature17625 · Cited by 928 · see citing works
Syk kinase signalling couples to the Nlrp3 inflammasome for anti-fungal host defence
Groß, O., Poeck, H., Bscheider, M., Dostert, C., Hannesschläger, N., Endres, S., Hartmann, G., Tardivel, A., Schweighoffer, E., Tybulewicz, V. L. J., et al. (2009) · Nature · doi:10.1038/nature07965 · Cited by 922 · see citing works
Candida albicans morphogenesis and host defence: discriminating invasion from colonization
Gow, N. A. R., van de Veerdonk, F. L., Brown, A. J. P., & Netea, M. G. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2711 · Cited by 888 · see citing works
Keeping It All Going—Complement Meets Metabolism
Kolev, M. V., & Kemper, C. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.00001 · Cited by 819 · see citing works
Enzymatic Assembly of Overlapping DNA Fragments
Gibson, D. G. (2011) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-385120-8.00015-2 · Cited by 741 · see citing works
Sugiyama, S., Okada, Y., Sukhova, G. K., Virmani, R., Heinecke, J. W., & Libby, P. (2001) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64036-9 · Cited by 699 · see citing works
Dectin‐1 mediates macrophage recognition of Candida albicans yeast but not filaments
Gantner, B. N., Simmons, R. M., & Underhill, D. M. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600594 · Cited by 671 · see citing works
Dectin-1 is required for host defense against Pneumocystis carinii but not against Candida albicans
Saijo, S., Fujikado, N., Furuta, T., Chung, S.-H., Kotaki, H., Seki, K., Sudo, K., Akira, S., Adachi, Y., Ohno, N., et al. (2006) · Nature Immunology · doi:10.1038/ni1425 · Cited by 612 · see citing works
Hise, A. G., Tomalka, J. A., Ganesan, S., Patel, K., Hall, B. A., Brown, G. D., & Fitzgerald, K. A. (2009) · Cell Host & Microbe · doi:10.1016/j.chom.2009.05.002 · Cited by 568 · see citing works
Lin, L., Ibrahim, A., Xu, X., Farber, J., Avanesian, V., Baquir, B., Fu, Y., French, S., Edwards, J., & Spellberg, B. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000703 · Cited by 479 · see citing works
Rappleye, C. A., Eissenberg, L. G., & Goldman, W. E. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609848104 · Cited by 419 · see citing works
Myeloperoxidase of the leucocyte of normal human blood. I. Content and localization
Schultz, J., & Kaminker, K. (1962) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(62)90321-1 · Cited by 403 · see citing works
Ras Signaling Is Required for Serum-Induced Hyphal Differentiation in Candida albicans
Feng, Q., Summers, E., Guo, B., & Fink, G. R. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.20.6339-6346.1999 · Cited by 347 · see citing works
Dynamic, Morphotype-Specific Candida albicans β-Glucan Exposure during Infection and Drug Treatment
Wheeler, R. T., Kombe, D., Agarwala, S. D., & Fink, G. R. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000227 · Cited by 309 · see citing works
Organ-Specific Innate Immune Responses in a Mouse Model of Invasive Candidiasis
Lionakis, M. S., Lim, J. K., Lee, C.-C. R., & Murphy, P. M. (2011) · Journal of Innate Immunity · doi:10.1159/000321157 · Cited by 302 · see citing works
Cutting Edge: <i>Candida albicans</i> Hyphae Formation Triggers Activation of the Nlrp3 Inflammasome
Joly, S., Ma, N., Sadler, J. J., Soll, D. R., Cassel, S. L., & Sutterwala, F. S. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0901323 · Cited by 296 · see citing works
Lactate signalling regulates fungal β-glucan masking and immune evasion
Ballou, E. R., Avelar, G. M., Childers, D. S., Mackie, J., Bain, J. M., Wagener, J., Kastora, S. L., Panea, M. D., Hardison, S. E., Walker, L. A., et al. (2016) · Nature Microbiology · doi:10.1038/nmicrobiol.2016.238 · Cited by 269 · see citing works
The Pathogen Candida albicans Hijacks Pyroptosis for Escape from Macrophages
Uwamahoro, N., Verma-Gaur, J., Shen, H.-H., Qu, Y., Lewis, R., Lu, J., Bambery, K., Masters, S. L., Vince, J. E., Naderer, T., et al. (2014) · mBio · doi:10.1128/mbio.00003-14 · Cited by 264 · see citing works
Global analysis of fungal morphology exposes mechanisms of host cell escape
O’Meara, T. R., Veri, A. O., Ketela, T., Jiang, B., Roemer, T., & Cowen, L. E. (2015) · Nature Communications · doi:10.1038/ncomms7741 · Cited by 243 · see citing works
Characterization and quantification of the peroxidase in human monocytes
Bos, A., Wever, R., & Roos, D. (1978) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(78)90197-3 · Cited by 238 · see citing works
Mice with Disseminated Candidiasis Die of Progressive Sepsis
Spellberg, B., Ibrahim, A. S., Edwards Jr., J. E., & Filler, S. G. (2005) · The Journal of Infectious Diseases · doi:10.1086/430952 · Cited by 230 · see citing works
Self-citationCandida albicans Triggers NLRP3-Mediated Pyroptosis in Macrophages
Wellington, M., Koselny, K., Sutterwala, F. S., & Krysan, D. J. (2014) · Eukaryotic Cell · doi:10.1128/ec.00336-13 · Cited by 227 · see citing works
Lane, S., Birse, C., Zhou, S., Matson, R., & Liu, H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104484200 · Cited by 220 · see citing works
Self-citationSherrington, S. L., Sorsby, E., Mahtey, N., Kumwenda, P., Lenardon, M. D., Brown, I., Ballou, E. R., MacCallum, D. M., & Hall, R. A. (2017) · PLOS Pathogens · doi:10.1371/journal.ppat.1006403 · Cited by 202 · see citing works
Mukaremera, L., Lee, K. K., Mora-Montes, H. M., & Gow, N. A. R. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.00629 · Cited by 202 · see citing works
Differential Adaptation of Candida albicans In Vivo Modulates Immune Recognition by Dectin-1
Marakalala, M. J., Vautier, S., Potrykus, J., Walker, L. A., Shepardson, K. M., Hopke, A. R., Mora-Montes, H. M., Kerrigan, A. M., Netea, M. G., Murray, G. I., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003315 · Cited by 201 · see citing works
(2019) · Nature reviews. Immunology · doi:10.1038/41577.1474-1741 · Cited by 201 · see citing works
Candida albicans hyphal initiation and elongation
Lü, Y., Su, C., & Liu, H. (2014) · Trends in Microbiology · doi:10.1016/j.tim.2014.09.001 · Cited by 195 · see citing works
Self-citationCheng, S.-C., van de Veerdonk, F. L., Lenardon, M., Stoffels, M., Plantinga, T., Smeekens, S., Rizzetto, L., Mukaremera, L., Preechasuth, K., Cavalieri, D., et al. (2011) · Journal of Leukocyte Biology · doi:10.1189/jlb.1210702 · Cited by 191 · see citing works
del Fresno, C., Soulat, D., Roth, S., Blazek, K. D., Udalova, I. A., Sancho, D., Ruland, J., & Ardavı́n, C. (2013) · Immunity · doi:10.1016/j.immuni.2013.05.010 · Cited by 189 · see citing works
Carrion, S. D. J., Leal, S. M., Ghannoum, M. A., Aimanianda, V., Latgé, J., & Pearlman, E. M. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1300748 · Cited by 187 · see citing works
Self-citationA Tetraploid Intermediate Precedes Aneuploid Formation in Yeasts Exposed to Fluconazole
Harrison, B. D., Hashemi, J., Bibi, M., Pulver, R., Bavli, D., Nahmias, Y., Wellington, M., Sapiro, G., & Berman, J. (2014) · PLoS Biology · doi:10.1371/journal.pbio.1001815 · Cited by 174 · see citing works
EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans
Swidergall, M., Solis, N. V., Lionakis, M. S., & Filler, S. G. (2017) · Nature Microbiology · doi:10.1038/s41564-017-0059-5 · Cited by 167 · see citing works
Majer, O., Bourgeois, C., Zwolanek, F., Lassnig, C., Kerjaschki, D., Mack, M., Müller, M., & Kuchler, K. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002811 · Cited by 156 · see citing works
Candida and Host Determinants of Susceptibility to Invasive Candidiasis
Lionakis, M. S., & Netea, M. G. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003079 · Cited by 149 · see citing works
Rosas, M., Liddiard, K., Kimberg, M., Faro‐Trindade, I., McDonald, J. U., Williams, D. L., Brown, G. D., & Taylor, P. R. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.5.3549 · Cited by 139 · see citing works
An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans
Nguyen, N., Quail, M. M., & Hernday, A. D. (2017) · mSphere · doi:10.1128/mspheredirect.00149-17 · Cited by 137 · see citing works
Pradhan, A., Avelar, G. M., Bain, J. M., Childers, D. S., Larcombe, D. E., Netea, M. G., Shekhova, E., Munro, C. A., Brown, G. D., Erwig, L. P., et al. (2018) · mBio · doi:10.1128/mbio.01318-18 · Cited by 133 · see citing works
Ganesan, S., Rathinam, V. A. K., Bossaller, L., Army, K., Kaiser, W. J., Mocarski, E. S., Dillon, C. P., Green, D. R., Mayadas, T. N., Levitz, S. M., et al. (2014) · The Journal of Immunology · doi:10.4049/jimmunol.1400276 · Cited by 131 · see citing works
Mitotic Exit and Separation of Mother and Daughter Cells
Weiss, E. L. (2012) · Genetics · doi:10.1534/genetics.112.145516 · Cited by 131 · see citing works
Mulhern, S. M., Logue, M. E., & Butler, G. (2006) · Eukaryotic Cell · doi:10.1128/ec.00155-06 · Cited by 130 · see citing works
Bi, L., Gojestani, S., Wu, W., Hsu, Y.-M. S., Zhu, J., Ariizumi, K., & Lin, X. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.131300 · Cited by 126 · see citing works
Non-canonical signalling mediates changes in fungal cell wall PAMPs that drive immune evasion
Pradhan, A., Avelar, G. M., Bain, J. M., Childers, D., Pelletier, C., Larcombe, D. E., Shekhova, E., Netea, M. G., Brown, G. D., Erwig, L., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13298-9 · Cited by 119 · see citing works
Lionakis, M. S., Fischer, B. G., Lim, J. K., Swamydas, M., Wan, W., Richard Lee, C.-C., Cohen, J. I., Scheinberg, P., Gao, J.-L., & Murphy, P. M. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002865 · Cited by 118 · cited 1× · see citing works
“To determine if the eng1 mutant elicited a different inflammatory response than the ENG1 complemented strain, we analyzed the levels of cytokines and chemokines that are upregulated in the kidney during systemic candidiasis [4].”
BackgroundSoluble Dectin-1 as a tool to detect β-glucans
Graham, L. M., Tsoni, S. V., Willment, J. A., Williams, D. L., Taylor, P. R., Gordon, S., Dennehy, K. M., & Brown, G. D. (2006) · Journal of Immunological Methods · doi:10.1016/j.jim.2006.05.013 · Cited by 114 · see citing works
Candidalysin Crucially Contributes to Nlrp3 Inflammasome Activation by Candida albicans Hyphae
Rogiers, O., Frising, U. C., Kucharíková, S., Jabra-Rizk, M. A., van Loo, G., Van Dijck, P., & Wullaert, A. (2019) · mBio · doi:10.1128/mbio.02221-18 · Cited by 113 · see citing works
The Eng1 β-Glucanase Enhances Histoplasma Virulence by Reducing β-Glucan Exposure
Garfoot, A. L., Shen, Q., Wüthrich, M., Klein, B. S., & Rappleye, C. A. (2016) · mBio · doi:10.1128/mbio.01388-15 · Cited by 99 · see citing works
Galán-Díez, M., Arana, D. M., Serrano‐Gómez, D., Kremer, L., Casasnovas, J. M., Ortega, M., Cuesta‐Domínguez, Á., Corbí, A. L., Plá, J., & Fernández‐Ruiz, E. (2010) · Infection and Immunity · doi:10.1128/iai.00989-09 · Cited by 98 · see citing works
The Damage-Response Framework of Microbial Pathogenesis and Infectious Diseases
Pirofski, L., & Casadevall, A. (2008) · Advances in experimental medicine and biology · doi:10.1007/978-0-387-09550-9_11 · Cited by 96 · see citing works
Targeting CBLB as a potential therapeutic approach for disseminated candidiasis
Xiao, Y., Tang, J., Guo, H., Zhao, Y., Tang, R., Ouyang, S., Zeng, Q., Rappleye, C. A., Rajaram, M. V. S., Schlesinger, L. S., et al. (2016) · Nature Medicine · doi:10.1038/nm.4141 · Cited by 92 · see citing works
Vendelė, I., Willment, J. A., Silva, L. M., Palma, A. S., Chai, W., Liu, Y., Feizi, T., Spyrou, M., Stappers, M. H. T., Brown, G. D., et al. (2020) · PLoS Pathogens · doi:10.1371/journal.ppat.1007927 · Cited by 80 · see citing works
Epitope Shaving Promotes Fungal Immune Evasion
Childers, D. S., Avelar, G. M., Bain, J. M., Pradhan, A., Larcombe, D. E., Netea, M. G., Erwig, L. P., Gow, N. A. R., & Brown, A. J. P. (2020) · mBio · doi:10.1128/mbio.00984-20 · Cited by 78 · see citing works
Amorim-Vaz, S., Tran, V. D. T., Pradervand, S., Pagni, M., Coste, A. T., & Sanglard, D. (2015) · mBio · doi:10.1128/mbio.00942-15 · Cited by 78 · see citing works
MacCallum, D. M., Castillo, L., Brown, A. J. P., Gow, N. A. R., & Odds, F. C. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006420 · Cited by 76 · see citing works
Emerging trends in candidemia: A higher incidence but a similar outcome
Fortün, J., Martín‐Dávila, P., de la Pedrosa, E. G., Pintado, V., Cobo, J., Fresco, G., Meije, Y., Ros, L., Álvarez, M. E. D., Luengo, J. D. M. L., et al. (2012) · Journal of Infection · doi:10.1016/j.jinf.2012.02.011 · Cited by 75 · see citing works
Wang, A., Raniga, P. P., Lane, S., Lü, Y., & Liu, H. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.01502-08 · Cited by 75 · see citing works
Self-citationCottier, F., Sherrington, S. L., Cockerill, S., del Olmo, V., Kissane, S., Tournu, H., Orsini, L., Palmer, G. E., Pérez, J. C., & Hall, R. A. (2019) · mBio · doi:10.1128/mbio.02347-19 · Cited by 64 · see citing works
Znaidi, S., Nesseir, A., Chauvel, M., Rossignol, T., & d’Enfert, C. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003519 · Cited by 58 · see citing works
Esteban, P. F., Ríos-Serrano, I., García, R., Dueñas, E., Plá, J., Sánchez, M., de Aldana, C. R. V., & del Rey, F. (2005) · Current Microbiology · doi:10.1007/s00284-005-0066-2 · Cited by 57 · see citing works
Exposure of Candida albicans β (1,3)-glucan is promoted by activation of the Cek1 pathway
Chen, T., Jackson, J. W., Tams, R. N., Davis, S. E., Sparer, T. E., & Reynolds, T. B. (2019) · PLOS Genetics · doi:10.1371/journal.pgen.1007892 · Cited by 56 · see citing works
Sem, X., Le, G. T. T., Tan, A. S. M., Tso, G. H. W., Yurieva, M., Liao, W. W. P., Lum, J., Srinivasan, K. G., Poidinger, M., Zolezzi, F., et al. (2016) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2016.00186 · Cited by 52 · see citing works
Thompson, A., Davies, L. C., Liao, C., da Fonseca, D. M., Griffiths, J. S., Andrews, R., Jones, A. V., Clement, M., Brown, G. D., Humphreys, I. R., et al. (2019) · PLoS Pathogens · doi:10.1371/journal.ppat.1007850 · Cited by 51 · see citing works
Naseem, S., Min, K., Spitzer, D., Gardin, J., & Konopka, J. B. (2017) · Genetics · doi:10.1534/genetics.117.201491 · Cited by 49 · see citing works
The interplay between inflammasome activation and antifungal host defense
van de Veerdonk, F. L., Joosten, L. A. B., & Netea, M. G. (2015) · Immunological Reviews · doi:10.1111/imr.12280 · Cited by 49 · see citing works
Epitope shaving promotes fungal immune evasion
Childers, D. S., Avelar, G. M., Bain, J. M., Pradhan, A., Larcombe, D. E., Netea, M. G., Erwig, L. P., Gow, N. A. R., & Brown, A. J. P. (2025) · Figshare · Cited by 46 · see citing works
Zhang, S. Q., Zou, Z., Shen, H., Shuai, S. S., Miao, Q., Huang, X., Liu, W., Li, L. P., Chen, S. M., Yan, L., et al. (2016) · PLoS Pathogens · doi:10.1371/journal.ppat.1005617 · Cited by 41 · see citing works
Lin, J., Wester, M. J., Graus, M. S., Lidke, K. A., & Neumann, A. K. (2016) · Molecular Biology of the Cell · doi:10.1091/mbc.e15-06-0355 · Cited by 36 · see citing works
Lrg1 Regulates β (1,3)-Glucan Masking in Candida albicans through the Cek1 MAP Kinase Pathway
Chen, T., Wagner, A. S., Tams, R. N., Eyer, J. E., Kauffman, S. J., Gann, E. R., Fernandez, E. J., & Reynolds, T. B. (2019) · mBio · doi:10.1128/mbio.01767-19 · Cited by 33 · see citing works
Granger, B. L. (2018) · PLoS ONE · doi:10.1371/journal.pone.0191194 · Cited by 31 · see citing works
Lionakis, M. S., Albert, N. D., Swamydas, M., Lee, C. R., Loetscher, P., & Kontoyiannis, D. P. (2016) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02365-16 · Cited by 19 · see citing works
Li, R. Q., Puri, S., Tati, S., Cullen, P. J., & Edgerton, M. (2015) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00214-15 · Cited by 18 · see citing works
Arroyo-Mendoza, M., Peraza, K., Olson, J. A., Adler‐Moore, J., & Buckley, N. E. (2020) · Heliyon · doi:10.1016/j.heliyon.2020.e04437 · Cited by 15 · see citing works
Trimming Surface Sugars Protects Histoplasma from Immune Attack
Brown, G. D. (2016) · mBio · doi:10.1128/mbio.00553-16 · Cited by 5 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [16]Lin J, Wester MJ, Graus MS, Lidke KA, Neumann AK. Nanoscopic cell-wall architecture of an immunogenic ligand in Candida albicans during antifungal drug treatment. Lippincott-Schwartz J, editor. Mol Biol Cell. 2016; 27: 1002– 1014. doi: 10.1091/mbc.E15-06-0355 26792838 PMC4791122 · DOI
Cited by
Sorted by citations
Candida albicans and Candida glabrata : global priority pathogens
Katsipoulaki, M., Stappers, M. H. T., Malavia, D., Brunke, S., Hube, B., & Gow, N. A. R. (2024) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00021-23 · Cited by 174 · see citing works
Nature of β-1,3-Glucan-Exposing Features on Candida albicans Cell Wall and Their Modulation
de Assis, L. J., Bain, J. M., Liddle, C., Leaves, I., Hacker, C., da Silva, R. P., Yuecel, R., Bebes, A., Stead, D., Childers, D. S., et al. (2022) · mBio · doi:10.1128/mbio.02605-22 · Cited by 69 · see citing works
Wang, H., Lu, Z., Keyhani, N. O., Deng, J., Zhao, X., Huang, S., Luo, Z., Jin, K., & Zhang, Y. (2023) · PLoS Pathogens · doi:10.1371/journal.ppat.1011578 · Cited by 55 · see citing works
Fungal resilience and host–pathogen interactions: Future perspectives and opportunities
Brown, A. J. P. (2022) · Parasite Immunology · doi:10.1111/pim.12946 · Cited by 40 · see citing works
T cell responses to control fungal infection in an immunological memory lens
Sharma, J., Mudalagiriyappa, S., & Nanjappa, S. G. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.905867 · Cited by 38 · see citing works
Jenull, S., Shivarathri, R., Tsymala, I., Penninger, P., Trinh, P.-C., Nogueira, F., Chauhan, M., Singh, A., Petryshyn, A., Stoiber, A., et al. (2022) · mBio · doi:10.1128/mbio.00799-22 · Cited by 32 · see citing works
Manipulation of host phagocytosis by fungal pathogens and therapeutic opportunities
Jia, L., González, K., Orasch, T., Schmidt, F., & Brakhage, A. A. (2024) · Nature Microbiology · doi:10.1038/s41564-024-01780-0 · Cited by 31 · see citing works
Fungal cell walls: the rising importance of carbohydrate-active enzymes.
Yao, R. A., Berrin, J., McKee, L., & Bissaro, B. (2025) · Trends in Microbiology · doi:10.1016/j.tim.2025.05.001 · Cited by 27 · see citing works
β-1,6-Glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall
Bekirian, C., Valsecchi, I., Bachellier-Bassi, S., Scandola, C., Guijarro, J. I., Chauvel, M., Mourer, T., Gow, N. A., Aimanianda, V. K., d'Enfert, C., et al. (2024) · eLife · doi:10.7554/elife.100569.3 · Cited by 28 · see citing works
Avelar, G. M., Dambuza, I. M., Ricci, L., Yuecel, R., Mackenzie, K. S., Childers, D. S., Bain, J. M., Pradhan, A., Larcombe, D. E., Netea, M. G., et al. (2022) · The Cell Surface · doi:10.1016/j.tcsw.2022.100084 · Cited by 25 · see citing works
Chen, T., Wagner, A. S., & Reynolds, T. B. (2022) · Frontiers in Fungal Biology · doi:10.3389/ffunb.2022.842501 · Cited by 27 · see citing works
Wagner, A. S., Lumsdaine, S. W., Mangrum, M. M., & Reynolds, T. B. (2023) · mBio · doi:10.1128/mbio.00074-23 · Cited by 26 · see citing works
DectiSomes: Glycan Targeting of Liposomal Drugs Improves the Treatment of Disseminated Candidiasis
Ambati, S., Pham, T., Lewis, Z., Lin, X., & Meagher, R. B. (2021) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01467-21 · Cited by 25 · see citing works
DectiSomes: C-type lectin receptor-targeted liposomes as pan-antifungal drugs
Meagher, R. B., Lewis, Z., Ambati, S., & Lin, X. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114776 · Cited by 24 · see citing works
Shivarathri, R., Chauhan, M., Datta, A., Das, D., Karuli, A., Aptekmann, A., Jenull, S., Kuchler, K., Thangamani, S., Chowdhary, A., et al. (2024) · mBio · doi:10.1128/mbio.02748-24 · Cited by 22 · see citing works
Wagner, A. S., Hancock, T. J., Lumsdaine, S. W., Kauffman, S., Mangrum, M. M., Phillips, E. K., Sparer, T. E., & Reynolds, T. B. (2021) · PLoS Pathogens · doi:10.1371/journal.ppat.1009839 · Cited by 21 · see citing works
Hyphal Als proteins act as CR3 ligands to promote immune responses against Candida albicans
Zhou, T., Solis, N. V., Marshall, M., Yao, Q., Garleb, R., Yang, M., Pearlman, E., Filler, S. G., & Liu, H. (2024) · Nature Communications · doi:10.1038/s41467-024-48093-8 · Cited by 21 · see citing works
Self-citationSelisana, S. M. G., Chen, X., Mahfudhoh, E., Bowolaksono, A., Rozaliyani, A., Orihara, K., & Kajiwara, S. (2024) · Medical Microbiology and Immunology · doi:10.1007/s00430-024-00795-y · Cited by 20 · see citing works
Hameed, S., Hans, S., Singh, S., Dhiman, R., Monasky, R. C., Pandey, R. P., Thangamani, S., & Fatima, Z. (2021) · Pathogens · doi:10.3390/pathogens10080942 · Cited by 17 · see citing works
Deng, J., Huang, S., Kan, Y., Song, Y., Zhao, X., Li, N., Yao, X., Luo, Z., & Zhang, Y. (2024) · mBio · doi:10.1128/mbio.03504-23 · Cited by 15 · see citing works
A CO <sub>2</sub> sensing module modulates β-1,3-glucan exposure in <i>Candida albicans</i>
Avelar, G. M., Pradhan, A., Ma, Q., Hickey, E., Leaves, I., Liddle, C., Rodriguez Rondon, A. V., Kaune, A.-K., Shaw, S., Maufrais, C., et al. (2024) · mBio · doi:10.1128/mbio.01898-23 · Cited by 15 · see citing works
Gomomo, Z., Fanadzo, M., Mewa-Ngongang, M., Chidi, B. S., Hoff, J. W., van der Rijst, M., Mokwena, L., Setati, M. E., & du Plessis, H. W. (2025) · Journal of Fungi · doi:10.3390/jof11010026 · Cited by 14 · see citing works
Cek1 regulates ß(1,3)-glucan exposure through calcineurin effectors in Candida albicans
Wagner, A. S., Lumsdaine, S. W., Mangrum, M. M., King, A. E., Hancock, T. J., Sparer, T. E., & Reynolds, T. B. (2022) · PLOS Genetics · doi:10.1371/journal.pgen.1010405 · Cited by 12 · see citing works
Davis, F. A., Singh, K., Krampen, J. M., Rapala, J. R., Bryant, J. A., McFadden, M. J., Ost, K. S., Esher, S. K., Balunas, M. J., Wang, T., et al. (2026) · Current Biology · doi:10.1016/j.cub.2025.12.029 · Cited by 13 · see citing works
Bae, W., Lee, Y. J., Jung, W., Shin, S. L., Kim, T., & Sohn, M. (2023) · Scientific Reports · doi:10.1038/s41598-023-39454-2 · Cited by 12 · see citing works
Ma, Q., Pradhan, A., Leaves, I., Hickey, E., Roselletti, E., Dambuza, I. M., Larcombe, D. E., de Assis, L. J., Wilson, D., Erwig, L. P., et al. (2024) · The Cell Surface · doi:10.1016/j.tcsw.2024.100128 · Cited by 9 · see citing works
Teng, W., Subsomwong, P., Narita, K., Nakane, A., & Asano, K. (2024) · Cells · doi:10.3390/cells13020127 · Cited by 8 · see citing works
Yeast β-glucan promotes antiviral type I interferon response via dectin-1
Wang, J., Jin, X., Yan, S., Zhao, H., Pang, D., Ouyang, H., & Tang, X. (2024) · Veterinary Microbiology · doi:10.1016/j.vetmic.2024.110107 · Cited by 8 · see citing works
Wagner, A. S., Vogel, A. K., Lumsdaine, S. W., Phillips, E. K., Willems, H. M. E., Peters, B. M., & Reynolds, T. B. (2022) · Infection and Immunity · doi:10.1128/iai.00342-22 · Cited by 8 · see citing works
Wang, H., Song, G.-M., Li, X.-N., Huo, P., Huang, S., Lu, Z.-Y., Qin, X., Luo, Z.-B., & Zhang, Y.-J. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c06747 · Cited by 5 · see citing works
Protein kinase A signaling regulates immune evasion by shaving and concealing fungal β-1,3-glucan
Pradhan, A., Nev, O. A., Leaves, I., Nev, O. A., Ma, Q., Milne, G., Patterson, G., Netea, M. G., Erwig, L. P., Farrer, R. A., et al. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2423864122 · Cited by 6 · see citing works
Kulig, K., Rudolphi-Skórska, E., Barbasz, A., Surowiec, M., Wronowska, E., Kowalik, K., Satała, D., Braś, G., Woźnicka, O., Karnas, E., et al. (2025) · Microbiology · doi:10.1099/mic.0.001565 · Cited by 6 · see citing works
Baicalin promotes β-1,3-glucan exposure in Candida albicans and enhances macrophage response
Pan, Y., Shi, Z., Wang, Y., Chen, F., Yang, Y., Ma, K., & Li, W. (2024) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2024.1487173 · Cited by 6 · see citing works
Lim, S. J., Noor, N. D. M., Sabri, S., Ali, M. S. M., Salleh, A. B., & Oslan, S. N. (2023) · Medical Mycology · doi:10.1093/mmy/myad126 · Cited by 6 · see citing works
Soto, N. M. M., Horn, A. J., Keller, N. P., Huttenlocher, A., & Wagner, A. S. (2025) · mBio · doi:10.1128/mbio.03480-24 · Cited by 5 · see citing works
Functional characterization of endo-β-1,3-glucanase in Trichoderma reesei
Wang, H., Chen, P., Wang, Y., Khan, B., Pang, A., Wu, F., & Lin, F. (2025) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2025.104022 · Cited by 5 · see citing works
Xin, C., Wang, F., Zhang, J., Zhou, Q., Liu, F., Zhao, C., & Song, Z. (2023) · The Journal of Microbiology · doi:10.1007/s12275-022-00007-3 · Cited by 5 · see citing works
Zhao, Y., Zou, W., Su, C., & Lu, Y. (2026) · iFungi · doi:10.26599/ifungi.2026.9670011 · Cited by 4 · see citing works
Sah, S. K., Yadav, A., Kruppa, M., & Rustchenko, E. P. (2023) · Microbiology Spectrum · doi:10.1128/spectrum.03295-23 · Cited by 5 · see citing works
Cohen, S., Crossen, A. J., Jensen, O., Neely, M. N., Doaré, K. L., Nobbs, A. H., Ost, K. S., & Doran, K. S. (2026) · Cell Host & Microbe · doi:10.1016/j.chom.2026.04.013 · Cited by 4 · see citing works
Wei, Q. (2025) · Carbohydrate Research · doi:10.1016/j.carres.2025.109715 · Cited by 3 · see citing works
Zhang, Q., Li, G., Wang, Y., Yang, C., Bai, W., Li, Q., Zhang, J., & Zhang, P. (2025) · Microorganisms · doi:10.3390/microorganisms13030683 · Cited by 2 · see citing works
Choi, W., Li, D.-D., McLaughlin, C. T., Peroumal, D., Shiomitsu, K. P., Moschetta, G. A., Rahimi, H., Wang, S., Biswas, K., Xie, D., et al. (2026) · Cell Host & Microbe · doi:10.1016/j.chom.2026.02.015 · Cited by 2 · see citing works
Henry, M., Khouas, M., Théberge-Julien, G., Vincent, A. T., Villeneuve, L. R., Rhéaume, É., Tardif, J., & Sellam, A. (2025) · Virulence · doi:10.1080/21505594.2025.2569630 · Cited by 1 · see citing works
Lactic Acid Influences Iron Assimilation by a Fungal Pathogen via the Iron Reductive Uptake Pathway
Gomes-Gonçalves, A., Genechten, W. V., Ataíde, P., Barata-Antunes, C., Ghasemi, F., Casal, M., Teixeira, M. C., Ariño, J., Brown, A. J. P., Dijck, P. V., et al. (2025) · MicrobiologyOpen · doi:10.1002/mbo3.70167 · Cited by 1 · see citing works
Human Dectin-1- and Dectin-2-targeted DectiSomes are effective against diverse pathogenic fungi
Ambati, S., Lin, X., Lewis, Z., PETER, J., & Meagher, R. B. (2026) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01689-25 · Cited by 1 · see citing works
Santos, S. R. D., Stempinski, P. R., Wieren, A. V., Gerbig, G. R., Smith, D. F. Q., Zamith‐Miranda, D., Nosanchuk, J. D., & Casadevall, A. (2026) · Microbiology Spectrum · doi:10.1128/spectrum.02897-25 · Cited by 1 · see citing works
Shared antibiofilm targets of biofilm regulators Wor3 and Bcr1 in Candida albicans
Goerlich, K., Solis, N. V., Filler, S. G., & Mitchell, A. P. (2026) · Genetics · doi:10.1093/genetics/iyag129 · Cited by 1 · see citing works
Self-citationXu, X., Jiang, B., Xiong, J., Jiang, Y., & Lu, H. (2026) · Vaccine · doi:10.1016/j.vaccine.2026.128864 · Cited by 1 · see citing works
Trentin, G., Martins‐Santana, L., Taborda, C. P., & Almeida, F. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1912476 · Cited by 1 · see citing works
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallPreprint
Bekirian, C., Valsecchi, I., Bachellier‐Bassi, S., Scandola, C., Guijarro, J. I., Chauvel, M., Mourer, T., Gow, N. A. R., Aimanianda, V., d’Enfert, C., et al. (2024) · eLife · doi:10.7554/elife.100569.1 · Cited by 1 · see citing works
Convergent evolution on RBA1 regulates Candidozyma auris host adaptationPreprint
Doorley, L. A., Bella, A. A. L., Jones, S. J., Zhao, G., Asadzadeh, M., Choudhury, Q. J., Ahmad, S., Mokaddas, E. M., Al‐Obaid, I., Alfouzan, W. A., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.04.692421 · Cited by 1 · see citing works
Bae, W., Lee, Y., Jung, W., Shin, S. L., Sohn, M., & Kim, T. (2022) · Research Square · doi:10.21203/rs.3.rs-2017122/v1
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallPreprint
Bekirian, C., Valsecchi, I., Bachellier‐Bassi, S., Scandola, C., Guijarro, J. I., Chauvel, M., Mourer, T., Gow, N. A. R., Aimanianda, V., d’Enfert, C., et al. (2024) · eLife · doi:10.7554/elife.100569.2
A conserved in vivo burn wound infection model for diverse pathogenic fungiPreprint
Soto, N. M. M., Horn, A. J., Keller, N. P., Anna Huttenlocher, A., & Wagner, A. S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.12.623264
King, A. E., Mangrum, M. M., Kauffman, S., Taylor, K. E., Arnold, D., Wagner, A. S., & Reynolds, T. B. (2026) · Infection and Immunity · doi:10.1128/iai.00523-25
Fonctions et biosynthèse des β-1,6-glucanes de la paroi cellulaire chez Candida albicans
Bekirian, C. (2024) · theses.fr (ABES) · doi:10.70675/b1028289z0c26z4acaz8f96z18e5c0c94464
Daudo, H. A., Marinho, E. S., de Oliveira, V. M., Marinho, M. M., & Ribeiro, R. V. (2026) · Molecular Diversity · doi:10.1007/s11030-026-11621-3
From confrontation to co-adaptation: the dynamic dialogue of Candida albicans and macrophages
Miramón, P., Wilson, H. B., & Lorenz, M. C. (2026) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00191-22
Peptide G3 Suppresses Hyphal Development in Candida albicans by Disrupting β-1,3-Glucan
Zhang, Y., Zhang, Y., Gu, C., Zhang, X., Jiang, X., Fan, X., Xu, H., & Chen, C. (2026) · Langmuir · doi:10.1021/acs.langmuir.6c02457
- Authors
- Yang, M., Solis, N. V., Marshall, M., Garleb, R., Zhou, T., Wang, D., Swidergall, M., Pearlman, E., Filler, S. G., & Liu, H.
- Affiliations
- University of California, Irvine (US)
- University of California, Los Angeles (US)
- Amgen (United States) (US)
- Corresponding author
- Liu, Haoping
- ORCID
- Venue
- PLOS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2022-01-07
- Volume
- 18
- Issue
- 1
- Pages
- e1010192
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- PMC
- PMC8775328
- OpenAlex
- W4206403554
- Cited by
- 62
- References
- 77
- Citations by year
- 2026: 13 · 2025: 12 · 2024: 17 · 2023: 7 · 2022: 9 · 2021: 4
- FWCI
- 5.50
- Type
- journal article
- Language
- en
- Update notices
- new_version (2022-01-20)
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS Pathogens (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM117111)
- National Institute of Allergy and Infectious Diseases (R01AI124566)
- National Institute of Dental and Craniofacial Research (R01DE026600)
- National Eye Institute (R01EY18612)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida albicans, Dectin-1, fungal virulence, hyphal morphogenesis, systemic candidiasis, sex differences in susceptibility, innate immune recognition, lactate, N-acetylglucosamine, septa
- MeSH terms
- Animals, Candida albicans, Candidiasis, Glucan Endo-1,3-beta-D-Glucosidase, Female, Male, Mice, Inbred C57BL, Virulence, beta-Glucans, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review