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The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
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There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details is required. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report addresses known requirements for dPCR that have already been identified during this early stage of its development and commercial implementation. Adoption of these guidelines by the scientific community will help to standardize experimental protocols, maximize efficient utilization of resources, and enhance the impact of this promising new technology.
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“As a first step toward this, guidelines for digital MIQE have been proposed (16).”
From Analysis of Extracellular RNA by Digital PCR (2014) · cites this paper as [16]
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Self-citation
Metadata
- Authors
- Huggett, J. F., Foy, C. A., Benes, V., Emslie, K., Garson, J. A., Haynes, R., Hellemans, J., Kubista, M., Mueller, R. D., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., Wittwer, C. T., & Bustin, S. A.
- Affiliations
- LGC (GB)
- Genomics Core Facility, EMBL Heidelberg, Heidelberg, Germany
- National Measurement Institute (AU)
- University College London (GB)
- National Institute of Standards and Technology (US)
- Ghent University Hospital (BE)
- Czech Academy of Sciences, Institute of Biotechnology (CZ)
- Sequenom Center for Molecular Medicine, San Diego, CA
- Sigma Custom Products, Haverhill, Suffolk, UK
- Physiology Weihenstephan, Center of Life and Food Sciences Weihenstephan, Germany
- Shipley Consulting, LLC, Houston, TX
- University of Utah (US)
- Anglia Ruskin University (GB)
- Corresponding author
- Huggett, Jim F
- ORCID
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-06-01
- Volume
- 59
- Issue
- 6
- Pages
- 892-902
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2099066165
- Cited by
- 859
- References
- 45
- Citations by year
- 2026: 47 · 2025: 47 · 2024: 50 · 2023: 56 · 2022: 78 · 2021: 95 · 2020: 96 · 2019: 67 · 2018: 71 · 2017: 59 · 2016: 74 · 2015: 58 · 2014: 48 · 2013: 11
- FWCI
- 28.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Anglia Ruskin Research Online (Anglia Ruskin University) (submittedVersion)
- ASEP (submittedVersion, public-domain)
- UCL Discovery (University College London) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- UK National Measurement System (AV0Z50520701, GACR GA P303/10/1338)
- BioFire Diagnostics
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Social Sciences › Decision Sciences › Statistics, Probability and Uncertainty › Meta-analysis and systematic reviews
- Keywords
- digital PCR, MIQE guidelines, reporting guidelines, experimental reproducibility, nucleic acid quantification, experimental design, control, standardization of protocols
- MeSH terms
- Computers, Guidelines as Topic, Real-Time Polymerase Chain Reaction
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