The DOI matches its Crossref record; 80 of the 80 references it cites were found in a registry or an index.
Delayed Stabilization of Dendritic Spines in Fragile X Mice
Open accessAbstract
Fragile X syndrome (FXS) causes mental impairment and autism through transcriptional silencing of the Fmr1 gene, resulting in the loss of the RNA-binding protein fragile X mental retardation protein (FMRP). Cortical pyramidal neurons in affected individuals and Fmr1 knock-out (KO) mice have an increased density of dendritic spines. The mutant mice also show defects in synaptic and experience-dependent circuit plasticity, which are known to be mediated in part by dendritic spine dynamics. We used in vivo time-lapse imaging with two-photon microscopy through cranial windows in male and female neonatal mice to test the hypothesis that dynamics of dendritic protrusions are altered in KO mice during early postnatal development. We find that layer 2/3 neurons from wild-type mice exhibit a rapid decrease in dendritic spine dynamics during the first 2 postnatal weeks, as immature filopodia are replaced by mushroom spines. In contrast, KO mice show a developmental delay in the downregulation of spine turnover and in the transition from immature to mature spine subtypes. Blockade of metabotropic glutamate receptor (mGluR) signaling, which reverses some adult phenotypes of KO mice, accentuated this immature protrusion phenotype in KO mice. Thus, absence of FMRP delays spine stabilization and dysregulated mGluR signaling in FXS may partially normalize this early synaptic defect.
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“These experiments revealed that in our xenotransplantation assay, human cortical neurons show increasingly stable dendritic spine dynamics, comparable to juvenile mouse cortical neurons”
From Xenotransplanted human cortical neurons reveal species-specific development and functional integration into mouse visual circuits (2019) · cites this paper as Cruz-Martín et al., 2010
In the citing paper
“Fmr1 mutant mice have a significant delay in the stabilization of dendritic spines and in the maturation of thalamocortical and corticocortical synapses 94-98 , defects associated with a temporal shift in the critical period for sensory-induced plasticity across different cortical areas 94,97,99-101 .”
From Developmental timing and critical windows for the treatment of psychiatric disorders (2016) · cites this paper as [95]
In the citing paper
“They also show a delayed maturation of spines [75]”
From Experience-dependent structural plasticity in the cortex (2011) · cites this paper as [75]
In the citing paper
“Both studies reveal a significant increase in spine turnover in the barrel cortex of Fmr1 KO mice.”
From Experience-dependent structural plasticity in the cortex (2011) · cites this paper as [75]
In the citing paper
“For example, spines on the apical dendrites of layer 2/3 pyramidal neurons in rodent barrel cortex exhibit gradually decreasing motility (elongation and shortening of spines) and turnover rate (defined as the total amount of gains and losses of spines) between postnatal day 7 and 24 (P7-24; Lendvai et al., 2000; Cruz-Martin et al., 2010).”
From Spatiotemporal dynamics of dendritic spines in the living brain (2014) · cites this paper as Cruz-Martin et al., 2010
In the citing paper
“Nevertheless, spine density continuously increases over this period of time (Cruz-Martin et al., 2010).”
From Spatiotemporal dynamics of dendritic spines in the living brain (2014) · cites this paper as Cruz-Martin et al., 2010
In the citing paper
“In vivo studies further showed that in such animals spine turnover increased in various cortical areas (Cruz-Martin et al., 2010; Pan et al., 2010; Padmashri et al., 2013), and neither whisker trimming nor motor learning could further alter spine dynamics (Pan et al., 2010; Padmashri et al., 2013).”
From Spatiotemporal dynamics of dendritic spines in the living brain (2014) · cites this paper as Cruz-Martin et al., 2010
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Citations
Cites
Structural basis of long-term potentiation in single dendritic spines
Matsuzaki, M., Honkura, N., Ellis-Davies, G. C. R., & Kasai, H. (2004) · Nature · doi:10.1038/nature02617 · Cited by 2,483
Experience-dependent structural synaptic plasticity in the mammalian brain
Holtmaat, A., & Svoboda, K. (2009) · Nature Reviews Neuroscience · doi:10.1038/nrn2699 · Cited by 1,934
The mGluR theory of fragile X mental retardation
Bear, M. F., Huber, K. M., & Warren, S. T. (2004) · Trends in Neurosciences · doi:10.1016/j.tins.2004.04.009 · Cited by 1,580
Matsuzaki, M., Ellis-Davies, G. C. R., Nemoto, T., Miyashita, Y., Iino, M., & Kasai, H. (2001) · Nature Neuroscience · doi:10.1038/nn736 · Cited by 1,563 · cited 2×
“Previous work has established a strong correlation between protrusion size and synaptic strength (Matsuzaki et al., 2001; Zito et al., 2009), and modulating synaptic strength results in changes in spine volume (Lang et al.”
BackgroundImpaired Spatial Learning in α-Calcium-Calmodulin Kinase II Mutant Mice
Silva, A. J., Paylor, R. E., Wehner, J. M., & Tonegawa, S. (1992) · Science · doi:10.1126/science.1321493 · Cited by 1,366
Altered synaptic plasticity in a mouse model of fragile X mental retardation
Huber, K. M., Gallagher, S. M., Warren, S. T., & Bear, M. F. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.122205699 · Cited by 1,349
ScanImage: Flexible software for operating laser scanning microscopes
Pologruto, T. A., Sabatini, B. L., & Svoboda, K. (2003) · BioMedical Engineering OnLine · doi:10.1186/1475-925x-2-13 · Cited by 1,300
Morphological Changes in Dendritic Spines Associated with Long-Term Synaptic Plasticity
Yuste, R., & Bonhoeffer, T. (2001) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.24.1.1071 · Cited by 1,269
Migaud, M., Charlesworth, P., Dempster, M., Webster, L. C., Watabe, A. M., Makhinson, M., He, Y., Ramsay, M. F., Morris, R. G. M., Morrison, J. H., et al. (1998) · Nature · doi:10.1038/24790 · Cited by 1,160
Transient and persistent dendritic spines in the neocortex in vivo
Holtmaat, A. J. G. D., Trachtenberg, J. T., Wilbrecht, L., Shepherd, G. M., Zhang, X., Knott, G. W., & Svoboda, K. (2005) · Neuron · doi:10.1016/j.neuron.2005.01.003 · Cited by 1,131
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Synaptic Pruning by Microglia Is Necessary for Normal Brain Development
Paolicelli, R. C., Bolasco, G., Pagani, F., Maggi, L., Scianni, M., Panzanelli, P., Giustetto, M., Ferreira, T. A., Guiducci, E., Dumas, L., et al. (2011) · Science · doi:10.1126/science.1202529 · Cited by 4,244
Autism spectrum disorder: neuropathology and animal models
Varghese, M., Keshav, N., Jacot-Descombes, S., Warda, T., Wicinski, B., Dickstein, D. L., Harony-Nicolas, H., De Rubeis, S., Drapeau, E., Buxbaum, J. D., et al. (2017) · Acta Neuropathologica · doi:10.1007/s00401-017-1736-4 · Cited by 536
Structural plasticity upon learning: regulation and functions
Caroni, P., Donato, F., & Muller, D. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3258 · Cited by 491
The translation of translational control by FMRP: therapeutic targets for FXS
Darnell, J. C., & Klann, E. (2013) · Nature Neuroscience · doi:10.1038/nn.3379 · Cited by 491
Spine Dynamics: Are They All the Same?
Berry, K., & Nedivi, E. (2017) · Neuron · doi:10.1016/j.neuron.2017.08.008 · Cited by 497
Metadata
- Authors
- Cruz-Martin, A., Crespo, M., & Portera-Cailliau, C.
- Affiliations
- Pediatrics and Genetics (US)
- Venue
- Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2010-06-09
- Volume
- 30
- Issue
- 23
- Pages
- 7793-7803
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC2903441
- OpenAlex
- W2012010559
- Cited by
- 321
- References
- 80
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 14 · 2023: 15 · 2022: 18 · 2021: 24 · 2020: 25 · 2019: 19 · 2018: 18 · 2017: 24 · 2016: 27 · 2015: 23 · 2014: 34 · 2013: 28 · 2012: 16
- FWCI
- 12.42
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- FRAXA Research Foundation
- National Institutes of Health
- National Institute of General Medical Sciences
- National Institute of Child Health and Human Development
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD054453)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
- Keywords
- fragile X syndrome, FMRP, Fmr1 knockout mice, spine dynamics, filopodia, mushroom spines, layer 2/3 pyramidal neurons, synaptic plasticity
- MeSH terms
- Animals, Animals, Newborn, Disease Models, Animal, Fragile X Syndrome, Male, Mice, Inbred C57BL, Pseudopodia, Pyridines, Receptors, Metabotropic Glutamate, Mice, Knockout, Excitatory Amino Acid Antagonists, Dendritic Spines, Mice, Fragile X Messenger Ribonucleoprotein 1, Fragile X Mental Retardation Protein
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