Regulation of brain-derived neurotrophic factor and nerve growth factor mRNA in primary cultures of hippocampal neurons and astrocytes
Open accessAbstract
Brain-derived neurotrophic factor (BDNF) and NGF are both expressed by neurons in the hippocampus. In previous studies, it has been demonstrated that both BDNF and NGF mRNA levels are regulated by neuronal activity. Upregulation is predominantly regulated by the glutamate (NMDA and non-NMDA receptors); downregulation, predominantly by the GABA system (Zafra et al., 1990, 1991). In neuronal cultures of the rat hippocampus, potassium depolarization and kainic acid-mediated increases in BDNF and NGF mRNA were eliminated in a dose-dependent manner by the calcium channel blocker nifedipine. Conversely, calcium ionophores (Bay-K8644 and ionomycin) augmented BDNF and NGF mRNA levels by a calmodulin-mediated mechanism. In view of the fact that many potential modulators (conventional transmitters and neuropeptides) of neuronal and astrocytic BDNF and NGF mRNA synthesis may act via the adenylate cyclase system, we studied the effect of forskolin, an activator of adenylate cyclase. Indeed, forskolin enhanced the effects of calcium ionophores and kainic acid on BDNF and NGF mRNA levels. Cytokines, such as interleukin-1 and transforming growth factor-beta 1, which have previously been shown to increase NGF mRNA markedly in astrocytes, were without effect on neuronal BDNF and NGF mRNA levels. In contrast to neuronal cultures, where the regulation of BDNF and NGF mRNA was generally very similar, the regulation in astrocytes was distinctly different. All the cytokines that produce a marked increase in NGF mRNA were without effect on astrocyte BDNF mRNA levels, which under basic conditions were below the detection limit. However, norepinephrine produced a marked elevation of BDNF mRNA in astrocytes, an effect that was further enhanced by glutamate receptor agonists.(ABSTRACT TRUNCATED AT 250 WORDS)
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
BDNF synthesis in the visual cortex is regulated by visual stimulation.
“The observation that BDNF synthesis in the visual cortex is regulated by visual stimulation made BDNF the prime candidate for this activity-dependent regulated factor [163,164,165].”
From Neurotrophic Factor BDNF, Physiological Functions and Therapeutic Potential in Depression, Neurodegeneration and Brain Cancer (2020) · cites this paper as [163]
Cites
Ernfors, P., Wetmore, C., Olson, L., & Persson, H. (1990) · Neuron · doi:10.1016/0896-6273(90)90090-3 · Cited by 819 · see citing works
Isackson, P. J., Huntsman, M. M., Murray, K. D., & Gall, C. M. (1991) · Neuron · doi:10.1016/0896-6273(91)90234-q · Cited by 711 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Neurotrophins and Neuronal Plasticity
Thoenen, H. (1995) · Science · doi:10.1126/science.270.5236.593 · Cited by 1,927 · see citing works
Self-citationBrain-derived neurotrophic factor and its clinical Implications
Bathina, S., & Das, U. N. (2015) · Archives of Medical Science · doi:10.5114/aoms.2015.56342 · Cited by 1,381 · see citing works
NEUROTROPHINS AND SYNAPTIC PLASTICITY
McAllister, A. K., Katz, L. C., & Lo, D. C. (1999) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.22.1.295 · Cited by 1,337 · see citing works
Colucci-D’Amato, L., Speranza, L., & Volpicelli, F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21207777 · Cited by 991 · see citing works
BDNF and Activity-Dependent Synaptic Modulation: Figure 1.
Lu, B. (2003) · Learning & Memory · doi:10.1101/lm.54603 · Cited by 945 · see citing works
Murer, M. G., Yan, Q., & Raisman-Vozari, R. (2001) · Progress in Neurobiology · doi:10.1016/s0301-0082(00)00014-9 · Cited by 874 · see citing works
Pruunsild, P., Kazantseva, A., Aid, T., Palm, K., & Timmusk, T. (2007) · Genomics · doi:10.1016/j.ygeno.2007.05.004 · Cited by 699 · see citing works
Brain-derived neurotrophic factor is reduced in Alzheimer's disease
Connor, B., Young, D., Yan, Q., Faull, R. L. M., Synek, B., & Dragunow, M. (1997) · Molecular Brain Research · doi:10.1016/s0169-328x(97)00125-3 · Cited by 581 · see citing works
Vaidya, V. A., Marek, G. J., Aghajanian, G. K., & Duman, R. S. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-08-02785.1997 · Cited by 479 · see citing works
McLaughlin, T., Torborg, C. L., Feller, M. B., & O'Leary, D. D. M. (2003) · Neuron · doi:10.1016/s0896-6273(03)00790-6 · Cited by 422 · see citing works
Blood-brain barrier permeability and physical exercise
Małkiewicz, M. A., Szarmach, A., Sabisz, A., Cubała, W. J., Szurowska, E., & Winklewski, P. J. (2019) · Journal of Neuroinflammation · doi:10.1186/s12974-019-1403-x · Cited by 276 · see citing works
Guan, Z., & Fang, J. (2006) · Brain, Behavior, and Immunity · doi:10.1016/j.bbi.2005.04.005 · Cited by 247 · see citing works
The function of gut microbiota in immune-related neurological disorders: a review
Sittipo, P., Choi, J., Lee, S., & Lee, Y. K. (2022) · Journal of Neuroinflammation · doi:10.1186/s12974-022-02510-1 · Cited by 221 · see citing works
Yamamoto-Sasaki, M., Ozawa, H., Saito, T., Rösler, M., & Riederer, P. (1999) · Brain Research · doi:10.1016/s0006-8993(99)01220-2 · Cited by 198 · see citing works
Chen, M. J., Nguyen, T. V., Pike, C. J., & Russo-Neustadt, A. A. (2007) · Cellular Signalling · doi:10.1016/j.cellsig.2006.05.028 · Cited by 158 · see citing works
Chronic Stress Weakens Connectivity in the Prefrontal Cortex: Architectural and Molecular Changes.
Woo, E., Sansing, L. H., Arnsten, A. F. T., & Datta, D. (2021) · Chronic Stress · doi:10.1177/24705470211029254 · Cited by 159 · see citing works
Hing, B., Sathyaputri, L., & Potash, J. B. (2018) · American Journal of Medical Genetics Part B: Neuropsychiatric Genetics · doi:10.1002/ajmg.b.32616 · Cited by 137 · see citing works
Fahimi, A., Baktir, M. A., Moghadam, S., Mojabi, F. S., Sumanth, K., McNerney, M. W., Ponnusamy, R., & Salehi, A. (2017) · Brain Structure and Function · doi:10.1007/s00429-016-1308-8 · Cited by 94 · see citing works
iPlasticity: Induced juvenile‐like plasticity in the adult brain as a mechanism of antidepressants
Umemori, J., Winkel, F., Didio, G., Llach Pou, M., & Castrén, E. (2018) · Psychiatry and Clinical Neurosciences · doi:10.1111/pcn.12683 · Cited by 80 · see citing works
Cheng, Q., & Yeh, H. H. (2003) · The Journal of Physiology · doi:10.1113/jphysiol.2002.037846 · Cited by 73 · see citing works
Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes
Takano, K., Yamasaki, H., Kawabe, K., Moriyama, M., & Nakamura, Y. (2012) · Journal of Pharmacological Sciences · doi:10.1254/jphs.12039fp · Cited by 70 · see citing works
Neurotoxicity of Aβ amyloid protein in vitro is not altered by calcium channel blockade
Whitson, J. S., & Appel, S. H. (1995) · Neurobiology of Aging · doi:10.1016/0197-4580(95)80002-9 · Cited by 41 · see citing works
- Authors
- Zafra, F., Lindholm, D., Castren, E., Hartikka, J., & Thoenen, H.
- Affiliations
- Max Planck Society (DE)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1992-12-01
- Volume
- 12
- Issue
- 12
- Pages
- 4793-4799
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6575775
- OpenAlex
- W1597356000
- Cited by
- 414
- References
- 2
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 7 · 2019: 8 · 2018: 6 · 2017: 15 · 2016: 9 · 2015: 12 · 2014: 8 · 2013: 14 · 2012: 9
- FWCI
- 8.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- brain-derived neurotrophic factor, nerve growth factor, mRNA regulation, hippocampal neurons, astrocytes, forskolin, adenylate cyclase, calcium ionophore, calmodulin, norepinephrine, glutamate receptors, kainic acid, nifedipine, cytokines, interleukin-1, TGF-β1, neuronal activity
- MeSH terms
- Cyclic AMP, Animals, Astrocytes, 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester, Calcium, Cells, Cultured, Colforsin, Glutamates, Growth Substances, Hippocampus, Isoquinolines, Kainic Acid, Kinetics, Nerve Growth Factors, Nerve Tissue Proteins, Neurons, Nifedipine, Norepinephrine, Piperazines, Potassium, RNA, Messenger, Tetradecanoylphorbol Acetate, Ionomycin, Cytokines, Quisqualic Acid, Glutamic Acid, Brain-Derived Neurotrophic Factor, 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, Protein Kinase Inhibitors, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
