Regulation of Dendritic Spine Morphogenesis by Insulin Receptor Substrate 53, a Downstream Effector of Rac1 and Cdc42 Small GTPases

Choi, Jeonghoon; Ko, Jaewon; Racz, Bence; Burette, Alain; Lee, Jae-Ran; Kim, Seho; Na, Moonseok; Lee, Hyun Woo; Kim, Karam; Weinberg, Richard J.; Kim, Eunjoon

doi:10.1523/jneurosci.3212-04.2005

Cited by 221

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Regulation of Dendritic Spine Morphogenesis by Insulin Receptor Substrate 53, a Downstream Effector of Rac1 and Cdc42 Small GTPases

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Choi, Jeonghoon, Ko, Jaewon, Racz, Bence, Burette, Alain, Lee, Jae-Ran, Kim, Seho, Na, Moonseok, Lee, Hyun Woo, Kim, Karam, Weinberg, Richard J., Kim, Eunjoon·2005·The Journal of Neuroscience

DOI 10.1523/jneurosci.3212-04.2005

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Abstract

The small GTPases Rac1 and Cdc42 are key regulators of the morphogenesis of actin-rich dendritic spines in neurons. However, little is known about how activated Rac1/Cdc42 regulates dendritic spines. Insulin receptor substrate 53 (IRSp53), which is highly expressed in the postsynaptic density (PSD), is known to link activated Rac1/Cdc42 to downstream effectors for actin regulation in non-neural cells. Here, we report that IRSp53 interacts with two specific members of the PSD-95 family, PSD-95 and chapsyn-110/PSD-93, in brain. An IRSp53 mutant lacking the C-terminal PSD-95-binding motif shows significant loss of synaptic localization in cultured neurons. Overexpression of IRSp53 in cultured neurons increases the density of dendritic spines but does not affect their length or width. Conversely, short-interfering RNA-mediated knock-down of IRSp53 reduces the density, length, and width of spines. In addition, the density and size of spines are decreased by a dominant-negative IRSp53 with a point mutation in the Src homology 3 (SH3) domain and a dominant-negative proline-rich region of WAVE2 (Wiskott-Aldrich syndrome protein family Verprolin-homologous protein), a downstream effector of IRSp53 that binds to the SH3 domain of IRSp53. These results suggest that PSD-95 interaction is an important determinant of synaptic IRSp53 localization and that the SH3 domain of IRSp53 links activated Rac1/Cdc42 to downstream effectors for the regulation of spine morphogenesis.

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Changing subunit composition of heteromeric NMDA receptors during development of rat cortex
Sheng, M., Cummings, J., Roldan, L. A., Jan, Y. N., & Jan, L. Y. (1994) · Nature · doi:10.1038/368144a0 · Cited by 1,377
PSD-95 Involvement in Maturation of Excitatory Synapses
El-Husseini, A. E.-D., Schnell, E., Chetkovich, D. M., Nicoll, R. A., & Bredt, D. S. (2000) · Science · doi:10.1126/science.290.5495.1364 · Cited by 1,303
Structure and function of dendritic spines
Nimchinsky, E. A., Sabatini, B. L., & Svoboda, K. (2002) · Annual Review of Physiology · doi:10.1146/annurev.physiol.64.081501.160008 · Cited by 1,208
Shank, a Novel Family of Postsynaptic Density Proteins that Binds to the NMDA Receptor/PSD-95/GKAP Complex and Cortactin
Naisbitt, S., Kim, E., Tu, J. C., Xiao, B., Sala, C., Valtschanoff, J., Weinberg, R. J., Worley, P. F., & Sheng, M. (1999) · Neuron · doi:10.1016/s0896-6273(00)80809-0 · Cited by 1,009
Self-citation
Actin-based plasticity in dendritic spines
Matus, A. (2000) · Science · doi:10.1126/science.290.5492.754 · Cited by 815
Activation of Arp2/3 complex-mediated actin polymerization by cortactin
Uruno, T., Liu, J., Zhang, P., Fan, Y.-X., Egile, C., Li, R., Mueller, S. C., & Zhan, X. (2001) · Nature Cell Biology · doi:10.1038/35060051 · Cited by 568 · cited 2×
“Cortactin, a Shank-associated protein that binds to F-actin and the Arp2/3 actin nucleation complex (Weed et al., 2000; Uruno et al., 2001), has been shown to induce the formation of protrusions in aspiny neurons and to be required for the maintenance of normal spine density, as demonstrated by siRNA downregulation (H…”
Background
Semiquantitative Proteomic Analysis of Rat Forebrain Postsynaptic Density Fractions by Mass Spectrometry
Peng, J., Kim, M. J., Cheng, D., Duong, D. M., Gygi, S. P., & Sheng, M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m400103200 · Cited by 473
Rapid Induction of Dendritic Spine Morphogenesis by trans-Synaptic EphrinB-EphB Receptor Activation of the Rho-GEF Kalirin
Penzes, P., Beeser, A., Chernoff, J., Schiller, M. R., Eipper, B. A., Mains, R. E., & Huganir, R. L. (2003) · Neuron · doi:10.1016/s0896-6273(02)01168-6 · Cited by 466
Identification of Two Human WAVE/SCAR Homologues as General Actin Regulatory Molecules Which Associate with the Arp2/3 Complex
Suetsugu, S., Miki, H., & Takenawa, T. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0894 · Cited by 246
The Shank Family of Postsynaptic Density Proteins Interacts with and Promotes Synaptic Accumulation of the βPIX Guanine Nucleotide Exchange Factor for Rac1 and Cdc42
Park, E., Na, M., Choi, J., Kim, S., Lee, J.-R., Yoon, J., Park, D., Sheng, M., & Kim, E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301052200 · Cited by 175 · cited 4×
“PSD-95 is also required for the synaptic localization of Kalirin-7 [Rac1 guanine nucleotide exchange factor (GEF)] (Penzes et al., 2001).”
BackgroundInfluentialSelf-citation

Show all 11 references

Research works printed but not linked: 53

1999, J Neurosci, 19, 7300, 10.1523/JNEUROSCI.19-17-07300.1999 · DOI · Paper page
10.1074/jbc.M202512200 · doi:10.1074/jbc.m202512200
10.1016/S0896-6273(00)80761-8 · DOI
10.1046/j.1471-4159.2002.01204.x · DOI
10.1016/0896-6273(92)90245-9 · DOI · Paper page
10.1074/jbc.M200528200 · DOI
10.1242/jcs.00617 · DOI
10.1016/S0028-3908(03)00267-3 · DOI
10.1006/bbrc.2000.2671 · DOI
10.1002/1096-9861(20001030)426:4<572::AID-CNE6>3.0.CO;2-9 · DOI · Paper page
Goslin K, Banker G (1991) Rat hippocampal neurons in low-density culture. In: Culturing nerve cells (Banker G, Goslin K, eds), pp 337-370. Cambridge, MA: MIT.
10.1083/jcb.152.3.579 · DOI · Paper page
10.1146/annurev.ne.17.030194.002013 · DOI · Paper page
10.1038/35104061 · DOI · Paper page
2003, J Neurosci, 23, 11759, 10.1523/JNEUROSCI.23-37-11759.2003 · DOI · Paper page
10.1016/S0014-5793(03)01074-3 · DOI
10.1016/S0896-6273(00)80319-0 · DOI · Paper page
10.1083/jcb.96.5.1374 · DOI · Paper page
10.1038/nn964 · DOI · Paper page
10.1038/nrn1517 · DOI · Paper page
10.1038/378085a0 · DOI · Paper page
10.1016/S0896-6273(00)80284-6 · DOI · Paper page
10.1016/S0960-9822(01)00506-1 · DOI · Paper page
10.1016/S0896-6273(00)81092-2 · DOI · Paper page
10.1016/S0896-6273(04)00108-4 · DOI · Paper page
10.1074/jbc.273.31.19518 · DOI · Paper page
10.1038/379837a0 · DOI · Paper page
10.1016/S0006-291X(02)00218-8 · DOI
10.1038/35047107 · DOI · Paper page
2002, J Neurosci, 22, 5253, 10.1523/JNEUROSCI.22-13-05253.2002 · DOI
2000, J Neurosci, 20, 5329, 10.1523/JNEUROSCI.20-14-05329.2000 · DOI · Paper page
2001, J Neurosci, 21, 6105, 10.1523/JNEUROSCI.21-16-06105.2001 · DOI · Paper page
10.1006/bbrc.2001.6102 · DOI
10.1126/science.1082475 · DOI · Paper page
10.1016/S0896-6273(01)00485-8 · DOI · Paper page
10.1074/jbc.275.9.6395 · DOI
10.1016/S0896-6273(01)00193-3 · DOI · Paper page
10.1523/JNEUROSCI.5479-03.2004 · DOI · Paper page
10.1016/S0896-6273(01)00339-7 · DOI · Paper page
2000, J Neurosci, 20, 1260, 10.1523/JNEUROSCI.20-03-01260.2000 · DOI · Paper page
2003, J Neurosci, 23, 3118, 10.1523/JNEUROSCI.23-08-03118.2003 · DOI
2003, J Neurosci, 23, 1310, 10.1523/JNEUROSCI.23-04-01310.2003 · DOI
2000, J Cell Sci, 113, 1851, 10.1242/jcs.113.11.1851 · DOI · Paper page
10.1146/annurev.neuro.24.1.1 · DOI · Paper page
10.1006/mcne.2002.1201 · DOI
10.1111/j.1471-4159.2004.02523.x · DOI
10.1093/cercor/10.10.927 · DOI · Paper page
10.1073/pnas.0335786100 · DOI
10.1083/jcb.151.1.29 · DOI · Paper page
10.1074/jbc.M309408200 · DOI · Paper page
10.1146/annurev.neuro.24.1.1071 · DOI · Paper page
10.1038/nrn1300 · DOI · Paper page
10.1083/jcb.200211002 · DOI · Paper page

The Postsynaptic Architecture of Excitatory Synapses: A More Quantitative View
Sheng, M., & Hoogenraad, C. C. (2007) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.76.060805.160029 · Cited by 1,003
The WASP-WAVE protein network: Connecting the membrane to the cytoskeleton.
Takenawa, T., & Suetsugu, S. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2069 · Cited by 947
Actin in dendritic spines: connecting dynamics to function
Hotulainen, P., & Hoogenraad, C. C. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201003008 · Cited by 815
Molecular mechanisms of dendritic spine morphogenesis
Tada, T., & Sheng, M. (2006) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2005.12.001 · Cited by 615
The Postsynaptic Organization of Synapses
Sheng, M., & Kim, E. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005678 · Cited by 603
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See all 221

Authors: Choi, J., Ko, J., Racz, B., Burette, A., Lee, J.-R., Kim, S., Na, M., Lee, H. W., Kim, K., Weinberg, R. J., & Kim, E.
Venue: The Journal of Neuroscience
Publisher: Society for Neuroscience
Published: 2005-01-26
Volume: 25
Issue: 4
Pages: 869-879
ISSN: 0270-6474 (ISSN-L)
1529-2401
DOI: 10.1523/jneurosci.3212-04.2005
OpenAlex: W2153951916
Cited by: 221
References: 11
FWCI: 4.42
Type: journal article
Language: en
Open access: hybrid
Licence: creativecommons.org/licenses/by-nc-sa/4.0/ (vor, 181 d)

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This page: CoChat. (2026). Citation check: Regulation of Dendritic Spine Morphogenesis by Insulin Receptor Substrate 53, a Downstream Effector of Rac1 and Cdc42 Small GTPases. https://app.cochat.ai/papers/10.1523/jneurosci.3212-04.2005
The paper: Choi, J., Ko, J., Racz, B., Burette, A., Lee, J.-R., Kim, S., Na, M., Lee, H. W., Kim, K., Weinberg, R. J., & Kim, E. (2005). Regulation of Dendritic Spine Morphogenesis by Insulin Receptor Substrate 53, a Downstream Effector of Rac1 and Cdc42 Small GTPases. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.3212-04.2005

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Regulation of Dendritic Spine Morphogenesis by Insulin Receptor Substrate 53, a Downstream Effector of Rac1 and Cdc42 Small GTPases

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