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BDNF Is Expressed in Skeletal Muscle Satellite Cells and Inhibits Myogenic Differentiation
Open accessAbstract
In skeletal muscle, brain-derived neurotrophic factor (BDNF) has long been thought to serve as a retrograde trophic factor for innervating motor neurons throughout their lifespan. However, its localization in mature muscle fibers has remained elusive. Given the postulated roles of BDNF in skeletal muscle, we performed a series of complementary experiments aimed at defining the localization of BDNF and its transcripts in adult muscle. By reverse transcription-PCR, in situ hybridization, and immunofluorescence, we show that BDNF, along with the receptor p75 NTR , is not expressed at significant levels within mature myofibers and that it does not accumulate preferentially within subsynaptic regions of neuromuscular junctions. Interestingly, expression of BDNF correlated with that of Pax3, a marker of muscle progenitor cells, in several different adult skeletal muscles. Additionally, BDNF was expressed in Pax7+ satellite cells where it colocalized with p75 NTR . In complementary cell culture experiments, we detected high levels of BDNF and p75 NTR in myoblasts. During myogenic differentiation, expression of BDNF became drastically reduced. Using small interfering RNA (siRNA) technology to knock down BDNF expression, we demonstrate enhanced myogenic differentiation of myoblasts. This accelerated rate of myogenic differentiation seen in myoblasts expressing BDNF siRNA was normalized by administration of recombinant BDNF. Collectively, these findings show that BDNF plays an important regulatory function during myogenic differentiation. In addition, the expression of BDNF in satellite cells is coherent with the notion that BDNF serves a key role in maintaining the population of muscle progenitors in adult muscle.
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“BDNF can also enter the brain via freely diffusing across the blood brain barrier (Pan et al., 1998; Mousavi and Jasmin, 2006).”
From Exercise-Mediated Neurogenesis in the Hippocampus via BDNF (2018) · cites this paper as Mousavi and Jasmin, 2006
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Metadata
- Authors
- Mousavi, K., & Jasmin, B. J.
- Affiliations
- University of Ottawa (CA)
- Corresponding author
- Jasmin, Bernard J.
- ORCID
- Jasmin, Bernard J.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2006-05-24
- Volume
- 26
- Issue
- 21
- Pages
- 5739-5749
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6675269
- OpenAlex
- W2029048316
- Cited by
- 210
- References
- 58
- Citations by year
- 2026: 10 · 2025: 16 · 2024: 20 · 2023: 9 · 2022: 20 · 2021: 13 · 2020: 11 · 2019: 11 · 2018: 9 · 2017: 11 · 2016: 14 · 2015: 10 · 2014: 10 · 2013: 13 · 2012: 3
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Canadian Institutes of Health Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Health Sciences › Medicine › Genetics (clinical) › Neurogenetic and Muscular Disorders Research
- Keywords
- brain-derived neurotrophic factor, skeletal muscle satellite cells, myogenesis, p75 neurotrophin receptor, Pax3, Pax7, muscle progenitor cells, myoblasts, neuromuscular junction, muscle regeneration
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Female, Tissue Distribution, Rats, Sprague-Dawley, Muscle, Skeletal, Brain-Derived Neurotrophic Factor, Receptor, Nerve Growth Factor, Satellite Cells, Skeletal Muscle, Rats
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