Cited by 118Mitochondrial Function and PathologyCardiac Ischemia and ReperfusionCell death mechanisms and regulation

Coordinated Behavior of Mitochondria in Both Space and Time: A Reactive Oxygen Species-Activated Wave of Mitochondrial Depolarization

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Brady, Nathan R., Elmore, Steven P., van Beek, Johannes J.H.G.M., Krab, Klaas, Courtoy, Pierre J., Hue, Louis, Westerhoff, Hans V.·2004·Biophysical Journal

Abstract

Reactive oxygen species (ROS) can trigger a transient burst of mitochondrial ROS production via ROS activation of the mitochondrial permeability transition pore (MPTP), a phenomenon termed ROS-induced ROS release (RIRR). The goal of this study was to investigate if the generation of ROS in a discrete region of a cardiomyocyte could serve to propagate RIRR-mediated mitochondrial depolarizations throughout a cell. Our experiments revealed that localized RIRR activated either RIRR-mediated fluctuations in mitochondrial membrane potential (time period: 3-10 min) or a traveling wave of depolarization of the cell's mitochondria (velocity: approximately 5 microm/min). Both phenomena appeared to be mediated by the mitochondrial permeability transition pore and eventually encompassed the majority of the mitochondrial population of both isolated rat and rabbit cardiomyocytes. Furthermore, depolarization was often reversible; the waves of depolarization were then followed by a rapid (approximately 40 microm/min) repolarization wave of the mitochondria. We show that the RIRR can function to communicate the mitochondrial permeability transition from one mitochondrion to another in the isolated adult cardiomyocyte.

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Crossref · 10.1529/biophysj.103.035097

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Authors
Brady, N. R., Elmore, S. P., van Beek, J. J. H. G. M., Krab, K., Courtoy, P. J., Hue, L., & Westerhoff, H. V.
Affiliations
  • Amsterdam Cardiovascular Sciences (NL)
  • UCLouvain (BE)
Corresponding author
Westerhoff, Hans V.
Venue
Biophysical Journal
Publisher
Elsevier BV
Published
2004-09
Volume
87
Issue
3
Pages
2022-2034
ISSN
0006-3495 (ISSN-L)
OpenAlex
W2112524117
Cited by
118
References
45
Citations by year
2026: 4 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 7 · 2016: 3 · 2015: 7 · 2014: 6 · 2013: 8 · 2012: 10
FWCI
2.13
Type
journal article
Language
en
Open access
Locations
  • Biophysical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion, public-domain)
  • VU Research Portal (submittedVersion, other-oa)
  • DIAL (Catholic University of Leuven) (submittedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Funders
  • Fédération Wallonie-Bruxelles
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Keywords
reactive oxygen species, mitochondrial permeability transition pore, mitochondrial depolarization, traveling waves, cardiomyocytes, mitochondrial membrane potential, mitochondrial reactive oxygen species, depolarization wave
MeSH terms
Animals, Calcium, Image Processing, Computer-Assisted, Kinetics, Light, Membrane Potentials, Mitochondria, Models, Biological, Rabbits, Rotenone, Time Factors, 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Reactive Oxygen Species, Microscopy, Confocal, Myocytes, Cardiac

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