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Cited by 298

The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.

A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice

Open access
Paul, Catriona, Murray, Alison A, Spears, Norah, Saunders, Philippa T K·2008·Reproduction
DOI 10.1530/rep-08-0036
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Abstract

Infertility represents a major clinical problem and 50% of cases are attributable to the male partner. Testicular function is temperature dependent, and in both man and mouse the position of the testes in the scrotum ensures that they are kept at between 2 and 8 °C below core body temperature. We used a mouse model to investigate the impact of a single, transient, mild, scrotal heat stress (38, 40 or 42 °C for 30 min) on testicular function, sperm DNA integrity and embryo survival. We detected temperature-dependent changes in testicular architecture, number of apoptotic cells and a significant reduction in testis weight 7 and 14 days after heat stress at 42 °C. We report for the first time that DNA strand breaks (γ-H2AX-positive foci) were present in spermatocytes recovered from testes subjected to 40 or 42 °C. Fertility of heat-stressed males was tested 23–28 d after treatment (sperm at this time would have been spermatocytes at time of heating). Paternal heat stress at 42 °C resulted in reduced pregnancy rate, placental weight and litter size; pregnancies from the 40 °C group had increased resorptions at e14.5. Abnormalities in embryonic development were detected at e3.5 and in vitro fertilisation with sperm recovered 16 h or 23 d after scrotal stress at 42 °C revealed a block in development between the 4-cell and blastocyst stages. This study has provided evidence of temperature-dependent effects on germ cell DNA integrity and highlighted the importance of an intact paternal genome for normal embryo development.

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Metadata

Authors
Paul, C., Murray, A. A., Spears, N., & Saunders, P. T. K.
Venue
Reproduction
Publisher
Oxford University Press (OUP)
Published
2008-07-01
Volume
136
Issue
1
Pages
73-84
ISSN
  • 1470-1626 (ISSN-L)
  • 1741-7899
DOI
10.1530/rep-08-0036
OpenAlex
W2013557046
Cited by
298
References
59
FWCI
11.88
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice. https://app.cochat.ai/papers/10.1530/rep-08-0036
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Paul, C., Murray, A. A., Spears, N., & Saunders, P. T. K. (2008). A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice. Reproduction. https://doi.org/10.1530/rep-08-0036

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.