DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae Encodes an RNA-Binding Protein from the Pentatricopeptide Repeat Family Required for the Maintenance of the Mitochondrial 15S Ribosomal RNA
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Pentatricopeptide repeat (PPR) proteins form the largest known RNA-binding protein family and are found in all eukaryotes, being particularly abundant in higher plants. PPR proteins localize mostly in mitochondria and chloroplasts, where they modulate organellar genome expression on the post-transcriptional level. The Saccharomyces cerevisiae DMR1 (CCM1, YGR150C) encodes a PPR protein that localizes to mitochondria. Deletion of DMR1 results in a complete and irreversible loss of respiratory capacity and loss of wild-type mtDNA by conversion to rho(-)/rho(0) petites, regardless of the presence of introns in mtDNA. The phenotype of the dmr1Delta mitochondria is characterized by fragmentation of the small subunit mitochondrial rRNA (15S rRNA), that can be reversed by wild-type Dmr1p. Other mitochondrial transcripts, including the large subunit mitochondrial rRNA (21S rRNA), are not affected by the lack of Dmr1p. The purified Dmr1 protein specifically binds to different regions of 15S rRNA in vitro, consistent with the deletion phenotype. Dmr1p is therefore the first yeast PPR protein, which has an rRNA target and is probably involved in the biogenesis of mitochondrial ribosomes and translation.
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Anikin, M., Henry, M. F., Hodorová, V., Houbaviy, H. B., Nosek, J., Pestov, D. G., & Markov, D. A. (2024) · RNA · doi:10.1261/rna.080254.124 · Cited by 3 · see citing works
Wenda, J. M., Drzewicka, K., Mulica, P., Tetaud, E., di Rago, J. P., Golik, P., & Łabędzka-Dmoch, K. (2024) · GENETICS · doi:10.1093/genetics/iyae124 · Cited by 2 · see citing works
Self-citationPentatricopeptide Repeat Domain-Containing Proteins in Mammals and Budding Yeast
Piva, M. A., & Moreno, J. I. (2012) · Cell & Developmental Biology · doi:10.4172/2168-9296.1000e108 · Cited by 1 · see citing works
Molecular analysis of PPR proteins in Chlamydomonas reinhardtii
Jalal, A. N. (2012) · Elektronische Hochschulschriften der LMU München (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.15080 · Cited by 1 · see citing works
Synthetic Pentatricopeptide Repeat Proteins: Building a Toolkit for Precise RNA Control
Lombana, J. M., Hanson, M. R., & Bentolila, S. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms262412033
Piva, M. A., Mayo, I., Chambers, J. A., Olivier, A. S., & Moreno, J. I. (2026) · Fermentation · doi:10.3390/fermentation12110506
- Authors
- Puchta, O., Lubas, M., Lipinski, K. A., Piatkowski, J., Malecki, M., & Golik, P.
- Affiliations
- Institute of Biochemistry and Biophysics, Polish Academy of Sciences (PL)
- Institute of Genetics and Biotechnology , Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland and
- University of Warsaw (PL)
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-04-01
- Volume
- 184
- Issue
- 4
- Pages
- 959-973
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- PMC
- PMC2865930
- OpenAlex
- W2138353295
- Cited by
- 33
- References
- 79
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 2 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 6
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- CCM1, pentatricopeptide repeat proteins, RNA-binding proteins, mitochondrial ribosome biogenesis, mitochondrial DNA maintenance, petite mutants, respiratory deficiency, Saccharomyces cerevisiae, rRNA binding
- MeSH terms
- Base Sequence, Cytoplasm, Exoribonucleases, Mitochondria, RNA, Ribosomal, Saccharomyces cerevisiae, Substrate Specificity, Gene Expression Regulation, Bacterial, RNA-Binding Proteins, Sequence Deletion, Cell Respiration, Amino Acid Motifs, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins
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