Cited by 33Mitochondrial Function and PathologyRNA Research and SplicingRNA and protein synthesis mechanisms

DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae Encodes an RNA-Binding Protein from the Pentatricopeptide Repeat Family Required for the Maintenance of the Mitochondrial 15S Ribosomal RNA

Open access
Puchta, Olga, Lubas, Michal, Lipinski, Kamil A, Piatkowski, Jakub, Malecki, Michal, Golik, Pawel·2010·Genetics

Abstract

Pentatricopeptide repeat (PPR) proteins form the largest known RNA-binding protein family and are found in all eukaryotes, being particularly abundant in higher plants. PPR proteins localize mostly in mitochondria and chloroplasts, where they modulate organellar genome expression on the post-transcriptional level. The Saccharomyces cerevisiae DMR1 (CCM1, YGR150C) encodes a PPR protein that localizes to mitochondria. Deletion of DMR1 results in a complete and irreversible loss of respiratory capacity and loss of wild-type mtDNA by conversion to rho(-)/rho(0) petites, regardless of the presence of introns in mtDNA. The phenotype of the dmr1Delta mitochondria is characterized by fragmentation of the small subunit mitochondrial rRNA (15S rRNA), that can be reversed by wild-type Dmr1p. Other mitochondrial transcripts, including the large subunit mitochondrial rRNA (21S rRNA), are not affected by the lack of Dmr1p. The purified Dmr1 protein specifically binds to different regions of 15S rRNA in vitro, consistent with the deletion phenotype. Dmr1p is therefore the first yeast PPR protein, which has an rRNA target and is probably involved in the biogenesis of mitochondrial ribosomes and translation.

Integrity signals

Registry

Crossref · 10.1534/genetics.110.113969

Cites

79 of 79 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Puchta, O., Lubas, M., Lipinski, K. A., Piatkowski, J., Malecki, M., & Golik, P.
Affiliations
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences (PL)
  • Institute of Genetics and Biotechnology , Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland and
  • University of Warsaw (PL)
Venue
Genetics
Published
2010-04-01
Volume
184
Issue
4
Pages
959-973
ISSN
  • 0016-6731 (ISSN-L)
  • 1943-2631
OpenAlex
W2138353295
Cited by
33
References
79
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 2 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 6
FWCI
1.82
Type
journal article
Language
en
Open access
Locations
  • Genetics (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
CCM1, pentatricopeptide repeat proteins, RNA-binding proteins, mitochondrial ribosome biogenesis, mitochondrial DNA maintenance, petite mutants, respiratory deficiency, Saccharomyces cerevisiae, rRNA binding
MeSH terms
Base Sequence, Cytoplasm, Exoribonucleases, Mitochondria, RNA, Ribosomal, Saccharomyces cerevisiae, Substrate Specificity, Gene Expression Regulation, Bacterial, RNA-Binding Proteins, Sequence Deletion, Cell Respiration, Amino Acid Motifs, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review