In vitro and in vivo antileishmanial activity of Artemisia annua L. leaf powder and its potential usefulness in the treatment of uncomplicated cutaneous leishmaniasis in humans
Open accessAbstract
INTRODUCTION:: Cutaneous leishmaniasis (CL) is a tropical disease that affects millions of individuals worldwide. The current drugs for CL may be effective but have serious side effects; hence, alternatives are urgently needed. Although plant-derived materials are used for the treatment of various diseases in 80% of the global population, the validation of these products is essential. Gelatin capsules containing dried Artemisia annua leaf powder were recently developed as a new herbal formulation (totum) for the oral treatment of malaria and other parasitic diseases. Here, we aimed to determine the usefulness of A. annua gel capsules in CL. METHODS:: The antileishmanial activity and cytotoxicity of A. annua L. capsules was determined via in vitro and in vivo studies. Moreover, a preliminary evaluation of its therapeutic potential as antileishmanial treatment in humans was conducted in 2 patients with uncomplicated CL. RESULTS:: Artemisia annua capsules showed moderate in vitro activity in amastigotes of Leishmania (Viannia) panamensis; no cytotoxicity in U-937 macrophages or genotoxicity in human lymphocytes was observed. Five of 6 (83.3%) hamsters treated with A. annua capsules (500mg/kg/day) for 30 days were cured, and the 2 examined patients were cured 45 days after initiation of treatment with 30g of A. annua capsules, without any adverse reactions. Both patients remained disease-free 26 and 24 months after treatment completion. CONCLUSION:: Capsules of A. annua L. represent an effective treatment for uncomplicated CL, although further randomized controlled trials are needed to validate its efficacy and safety.
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Self-citation
- Authors
- Mesa, L. E., Vasquez, D., Lutgen, P., Vélez, I. D., Restrepo, A. M., Ortiz, I., & Robledo, S. M.
- Affiliations
- Universidad de Antioquia (CO)
- Universidad de Antioquia, Colombia
- Iwerliewen Fir Bedreete Volleker-IFBV- Réseau belgo-luxembourgeois de valorisation des herbes médicinales-BELHERB, Luxembourg
- Universidad Pontificia Bolivariana (CO)
- Venue
- Revista da Sociedade Brasileira de Medicina Tropical
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2017-02
- Volume
- 50
- Issue
- 1
- Pages
- 52-60
- ISSN
- 0037-8682 (ISSN-L)
- 1678-9849
- OpenAlex
- W2596035464
- Cited by
- 39
- References
- 32
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 4 · 2020: 6 · 2019: 5 · 2018: 4 · 2017: 2 · 2016: 1
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by
- Locations
- Revista da Sociedade Brasileira de Medicina Tropical (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repository University of Antioquia (University of Antioquia) (publishedVersion, cc-by)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, cc-by-nc)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Greater South Information System
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Health Sciences › Medicine › Pharmacology › Leech Biology and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry Medicinal Plant Applications
- Keywords
- Artemisia annua, cutaneous leishmaniasis, antileishmanial activity, Leishmania (Viannia) panamensis, herbal medicine, gelatin capsules, amastigotes, cytotoxicity, genotoxicity, hamster model
- MeSH terms
- Adult, Animals, Antiprotozoal Agents, Female, Cricetinae, Humans, Leishmania, Male, Plant Extracts, Leishmaniasis, Cutaneous, Treatment Outcome, Plant Leaves, Parasitic Sensitivity Tests, Artemisia annua
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