The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
C-phycocyanin extraction from Spirulina platensis wet biomass
Open accessAbstract
C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymatic (lysozyme treatment) methods. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing (the method most frequently used), and presented a extraction yield of 43.75 mg.g-1 and a C-phycocyanin concentration of 0.21 mg.mL-1.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“It has been shown that the use of an ultrasonic water bath is the most effective way for extracting phycocyanin from spirulina algae. This method produced 43.75 mg/g of pigment at a concentration of 0.21 mg/mL [106].”
From Trends and Technological Advancements in the Possible Food Applications of Spirulina and Their Health Benefits: A Review. (2022) · cites this paper as [106]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
COMPLEMENTARY CHROMATIC ADAPTATION IN A FILAMENTOUS BLUE-GREEN ALGA
Bennett, A., & Bogorad, L. (1973) · The Journal of Cell Biology · doi:10.1083/jcb.58.2.419 · Cited by 1,773
C-Phycocyanin: A Biliprotein with Antioxidant, Anti-Inflammatory and Neuroprotective Effects
Romay, C., Gonzalez, R., Ledón, N., Remírez, D., & Rimbau, V. (2003) · Current Protein and Peptide Science · doi:10.2174/1389203033487216 · Cited by 718
C-Phycocyanin: A potent peroxyl radical scavenger in Vivo and in Vitro
Bhat, V. B., & Madyastha, K. M. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.3270 · Cited by 477
Antioxidant activity of different fractions of Spirulina platensis protean extract
Piñero Estrada, J. (2001) · Il Farmaco · doi:10.1016/s0014-827x(01)01084-9 · Cited by 447
Sarada, R., Pillai, M. G., & Ravishankar, G. A. (1999) · Process Biochemistry · doi:10.1016/s0032-9592(98)00153-8 · Cited by 442
HANDBOOK OF PHYCOLOGICAL METHODS–1973
(1975) · Journal of Phycology · doi:10.1111/j.1529-8817.1975.tb02783.x · Cited by 417
Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum
Román, R. B., Álvarez‐Pez, J. M., Acién, F. G., & Grima, E. M. (2002) · Journal of Biotechnology · doi:10.1016/s0168-1656(01)00385-6 · Cited by 314
Optimization of phycocyanin extraction from Spirulina platensis using factorial design
Silveira, S., Burkert, J. F. M., Costa, J. A. V., Burkert, C. A. V., & Kalil, S. J. (2006) · Bioresource Technology · doi:10.1016/j.biortech.2006.05.050 · Cited by 306
Bhat, V. B., & Madyastha, K. M. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.5195 · Cited by 277
C-phycocyanin as a storage protein in the blue-green alga Spirulina platensis
Boussiba, S., & RICHMOND, A. E. (1980) · Archives of Microbiology · doi:10.1007/bf00403211 · Cited by 222
Reddy, M. C., Subhashini, J., Mahipal, S. V. K., Bhat, V. B., Reddy, P. S., Kiranmai, G., Madyastha, K. M., & Reddanna, P. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(03)00586-2 · Cited by 208
Bhat, V. B., & Madyastha, K. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.5368 · Cited by 206
Abalde, J., Betancourt, L., Torres, E., Cid, Á., & Barwell, C. J. (1998) · Plant Science · doi:10.1016/s0168-9452(98)00113-7 · Cited by 204
Soni, B., Trivedi, U. B., & Madamwar, D. (2007) · Bioresource Technology · doi:10.1016/j.biortech.2006.11.010 · Cited by 148
Purification of Proteins and the Disruption of Microbial Cells
Kula, M., & Schütte, H. (1987) · Biotechnology Progress · doi:10.1002/btpr.5420030107 · Cited by 136
Protective effect of intracellular ice during freezing?
Acker, J. P., & McGann, L. E. (2003) · Cryobiology · doi:10.1016/s0011-2240(03)00025-7 · Cited by 119
Niu, J., Wang, G., & Tseng, C. (2006) · Protein Expression and Purification · doi:10.1016/j.pep.2006.02.001 · Cited by 105
Modelling of growth conditions for cyanobacterium Spirulina platensis in microcosms
Costa, J. A. V., Linde, G. A., Atala, D. I. P., Mibielli, G. M., & Krüger, R. T. (2000) · World Journal of Microbiology and Biotechnology · doi:10.1023/a:1008992826344 · Cited by 104
C-PHYCOCYANIN EXTRACTION PROCESS FOR LARGE-SCALE USE
Moraes, C. C., Burkert, J. F. D. M., & Kalil, S. J. (2010) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2009.00317.x · Cited by 97
Extraction, identification, and quantitation of phycobiliprotein pigments from phototrophic plankton
Stewart, D. E., & Farmer, F. H. (1984) · Limnology and Oceanography · doi:10.4319/lo.1984.29.2.0392 · Cited by 87
Cyanobacterial Phycobilisomes. Particles from synechocystis 6701 and two pigment mutants
Williams, R., Gingrich, J., & Glazer, A. N. (1980) · The Journal of Cell Biology · doi:10.1083/jcb.85.3.558 · Cited by 76
Cyanobacterial phycobilisomes. Role of the linker polypeptides in the assembly of phycocyanin.
Yu, M., & Glazer, A. N. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34795-1 · Cited by 75
Bermejo, R., Felipe, M. A., Talavera, E. M., & Álvarez‐Pez, J. M. (2006) · Chromatographia · doi:10.1365/s10337-005-0702-9 · Cited by 57
de Medeiros, F. O., Alves, F. G., Lisbôa, C. R., Martins, D. D. S., Burkert, C. A. V., & Kalil, S. J. (2008) · Química Nova · doi:10.1590/s0100-40422008000200028 · Cited by 34
Gacesa, P., & Hubble, J. (1990) · Dialnet (Universidad de la Rioja) · Cited by 10
Research works printed but not linked: 1
- Siegelman H. W., 1978, Algal biliproteins
Cited by
Sorted by citations
Phycobiliproteins from cyanobacteria: Chemistry and biotechnological applications
Pagels, F., Guedes, A. C., Amaro, H. M., Kijjoa, A., & Vasconcelos, V. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.02.010 · Cited by 426
Phycobiliprotein: Potential microalgae derived pharmaceutical and biological reagent
Manirafasha, E., Ndikubwimana, T., Zeng, X., Lu, Y., & Jing, K. (2016) · Biochemical Engineering Journal · doi:10.1016/j.bej.2016.01.025 · Cited by 321
Phycocyanin from Spirulina: A Review of Extraction Methods and Stability
Pez Jaeschke, D., Rocha Teixeira, I., Damasceno Ferreira Marczak, L., & Domeneghini Mercali, G. (2021) · Food Research International · doi:10.1016/j.foodres.2021.110314 · Cited by 254
AlFadhly, N. K. Z., Alhelfi, N., Altemimi, A. B., Verma, D. K., Cacciola, F., & Narayanankutty, A. (2022) · Molecules · doi:10.3390/molecules27175584 · Cited by 225
Prabakaran, G., Sampathkumar, P., Kavisri, M., & Moovendhan, M. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.03.009 · Cited by 215
Retracted
Metadata
- Authors
- Moraes, C. C., Sala, L., Cerveira, G. P., & Kalil, S. J.
- Affiliations
- Universidade Federal do Pampa (BR)
- Universidade Federal do Rio Grande (BR)
- Venue
- Brazilian Journal of Chemical Engineering
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2011-03
- Volume
- 28
- Issue
- 1
- Pages
- 45-49
- ISSN
- 0104-6632 (ISSN-L)
- OpenAlex
- W2113998511
- Cited by
- 187
- References
- 25
- Citations by year
- 2026: 9 · 2025: 11 · 2024: 25 · 2023: 18 · 2022: 23 · 2021: 16 · 2020: 22 · 2019: 16 · 2018: 19 · 2017: 10 · 2016: 8 · 2015: 4 · 2014: 3 · 2013: 3
- FWCI
- 2.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Brazilian Journal of Chemical Engineering (publishedVersion, cc-by-nc)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- C-phycocyanin, Spirulina platensis, phycocyanin extraction, wet biomass, ultrasound-assisted extraction, sonication, lysozyme treatment, acid treatment, freeze-thaw cycles, extraction yield
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
