Antioxidant activity of different fractions of Spirulina platensis protean extract
Abstract
Spirulina platensis, planktonic blue-green algae, is gaining increasing attention because of its nutritional and medicinal properties. This microalgae contains phycobiliproteins (phycocyanin and allophycocyanin). Previous reports from our laboratory have shown that a protean extract of S. platensis is a potent free-radical scavenger (hydroxyl and peroxyl radicals) and inhibits microsomal lipid peroxidation. The aim of this study was to purify and characterize phycocyanin of S. platensis. Besides, we tried to demonstrate that one of the main components responsible for this antioxidant activity is a biliprotein phycocyanin. For this purpose, we studied the antioxidant activity of different fractions obtained during the phycocyanin purification process, through the scavenger activity of hydroxyl radical. We also observed that an increase in phycocyanin content was related to an increase in the antioxidant activity in different fractions, and therefore phycobiliprotein phycocyanin is the component mainly responsible for the antioxidant activity.
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The antioxidant activity of spirulina is directly proportional to the quantity of phycocyanin.
“The antioxidant activity of spirulina has been proven to be directly proportional to the quantity of phycocyanin (which contains phycocyanobilin).12–14 45”
From Effects of spirulina on weight loss and blood lipids: A review (2020) · cites this paper as [45]
Phycocyanobilin is structurally similar to bilirubin and can inhibit NADPH oxidase.
“Phycocyanobilin is structurally similar to bilirubin and can inhibit NADPH oxidase.”
From Effects of spirulina on weight loss and blood lipids: A review (2020) · cites this paper as [45]
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- Authors
- Piñero Estrada, J.
- Venue
- Il Farmaco
- Publisher
- Elsevier BV
- Published
- 2001-07-01
- Volume
- 56
- Issue
- 5-7
- Pages
- 497-500
- ISSN
- 0014-827X (ISSN-L)
- OpenAlex
- W2122583090
- Cited by
- 447
- References
- 18
- Citations by year
- 2026: 11 · 2025: 11 · 2024: 20 · 2023: 21 · 2022: 28 · 2021: 24 · 2020: 32 · 2019: 23 · 2018: 33 · 2017: 17 · 2016: 18 · 2015: 30 · 2014: 20 · 2013: 16 · 2012: 20
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Il Farmaco (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- Spirulina platensis, phycocyanin, phycobiliproteins, antioxidant activity, hydroxyl radical scavenging, free radical scavenging, phycocyanin purification, microalgae, microsomal lipid peroxidation, cyanobacteria
- MeSH terms
- Cyanobacteria, Antioxidants, Ascorbic Acid, Chromatography, DEAE-Cellulose, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Hydrogen Peroxide, Iron, Molecular Weight, Phycocyanin, Free Radical Scavengers, Hydroxyl Radical
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