Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: Protection against oxidative damage to DNA
Abstract
Peroxynitrite (ONOO(-)) is known to inactivate important cellular targets and also mediate oxidative damage in DNA. The present study has demonstrated that phycocyanin, a biliprotein from spirulina platensis and its chromophore, phycocyanobilin (PCB), efficiently scavenge ONOO(-), a potent physiological inorganic toxin. Scavenging of ONOO(-) by phycocyanin and PCB was established by studying their interaction with ONOO(-) and quantified by using competition kinetics of pyrogallol red bleaching assay. The relative antioxidant ratio and IC(50) value clearly indicate that phycocyanin is a more efficient ONOO(-) scavenger than PCB. The present study has also shown that PCB significantly inhibits the ONOO(-)-mediated single-strand breaks in supercoiled plasmid DNA in a dose-dependent manner with an IC(50) value of 2.9 +/- 0.6 microM. These results suggest that phycocyanin, has the ability to inhibit the ONOO(-)-mediated deleterious biological effects and hence has the potential to be used as a therapeutic agent.
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Some cyanobacteria such as Arthrospira produce phycocyanin and its chromophore PCB, which act as potent antioxidants that inhibit peroxynitrite-mediated oxidative damage in DNA.
“From a crew radiation mitigation perspective, some cyanobacteria such as those from the genus Arthrospira offer the unique benefit of producing phycocyanin and its chromophore phycocyanobilin (PCB). These molecules act as potent antioxidants which inhibit peroxynitrite (ONOO–) mediated oxidative damage in DNA [107].”
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- Authors
- Bhat, V. B., & Madyastha, K. M.
- Affiliations
- Jawaharlal Nehru Centre for Advanced Scientific Research (IN)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2001-07
- Volume
- 285
- Issue
- 2
- Pages
- 262-266
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2087459960
- Cited by
- 277
- References
- 39
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 14 · 2023: 10 · 2022: 27 · 2021: 21 · 2020: 15 · 2019: 14 · 2018: 15 · 2017: 10 · 2016: 10 · 2015: 13 · 2014: 11 · 2013: 8 · 2012: 13
- FWCI
- 2.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- NOT FOUND REPOSITORY (Indian Institute of Science Bangalore) (acceptedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Jawaharlal Nehru Centre for Advanced Scientific Research
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- peroxynitrite, phycocyanin, phycocyanobilin, Spirulina platensis, free radical scavenging, oxidative DNA damage, single-strand breaks, plasmid DNA, antioxidant activity, biliproteins
- MeSH terms
- Cyanobacteria, DNA Damage, DNA, Superhelical, Kinetics, Nitrates, Phycocyanin, Plasmids, Pyrroles, Spectrophotometry, Free Radical Scavengers, Tetrapyrroles, Phycobilins
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