Effect of nitrogen limitation on cell growth, lipid accumulation and gene expression in Chlorella sorokiniana
Open accessAbstract
The influence of nitrogen (N) limitation on the cell growth, chlorophyll content, intracellular lipid production and expression levels of two pathway genes in Chlorella sorokiniana was investigated in mixotrophic culture in this study. The maximum biomass concentration of C. sorokinianacultured in modified BG11 medium containing 3, 6, 9, 12 and 15 mM N were 1.60, 2.21, 2.74, 3.18 and 3.21 g/L of dry cell weight, respectively with maximum specific growth rate of 0.180, 0.198, 0.201, 0.203, 0.206 day−1 during culture with an initial N feed of 3, 6, 9, 12 and 15 mM in the first eight days, respectively. The maximum lipid content was 51 % with 3 mM N. However, the maximum lipid productivity of 0.00884 g L−1 day−1 was achieved with 6 mM N. Expression levels of accD (heteromeric acetyl-CoA carboxylase beta subunit) and accI (homomeric acetyl-CoA carboxylase) genes in C. sorokiniana were studied by real-time PCR. Increased expression levels of accD reflected the increased lipid content in stationary phase. In contrast, expression of the acc1 gene always remained low, showing that the gene might not be critical for lipid accumulation.
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An increase in lipid content in response to nitrogen restriction is considered a general pattern.
“An increase in lipid content in response to nitrogen restriction is considered a general pattern [36, 80].”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [36]
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Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524 · see citing works
Xu, H., Miao, X., & Wu, Q. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.05.002 · Cited by 1,125 · see citing works
Li, Y., Horsman, M., Wang, B., Wu, N., & Lan, C. Q. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1681-1 · Cited by 1,116 · see citing works
Self-citationLiang, Y., Sarkany, N., & Cui, Y. (2009) · Biotechnology Letters · doi:10.1007/s10529-009-9975-7 · Cited by 1,059 · see citing works
Multi-subunit acetyl-CoA carboxylases
Cronan, J. E., & Waldrop, G. L. (2002) · Progress in Lipid Research · doi:10.1016/s0163-7827(02)00007-3 · Cited by 469 · see citing works
Sasaki, Y., & Nagano, Y. (2004) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.68.1175 · Cited by 463 · see citing works
Wan, M., Liu, P., Xia, J., Rosenberg, J. N., Oyler, G. A., Betenbaugh, M. J., Nie, Z., & Qiu, G. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3399-8 · Cited by 300 · see citing works
Sukenik, A., Zmora, O., & Carmeli, Y. (1993) · Aquaculture · doi:10.1016/0044-8486(93)90328-v · Cited by 265 · see citing works
Effect of C/N ratio and aeration on the fatty acid composition of heterotrophicChlorella sorokiniana
Chen, F., & Johns, M. R. (1991) · Journal of Applied Phycology · doi:10.1007/bf00003578 · Cited by 224 · see citing works
Cheng, Y., Zhou, W., Gao, C., Lan, K., Gao, Y., & Wu, Q. (2009) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2111 · Cited by 194 · see citing works
Liu, J., Yuan, C., Hu, G., & Li, F. (2012) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-9639-2 · Cited by 193 · see citing works
Sukenik, A., & Wahnon, R. (1991) · Aquaculture · doi:10.1016/0044-8486(91)90279-g · Cited by 155 · see citing works
Huang, X., Huang, Z., Wen, W., & Yan, J. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-012-9846-9 · Cited by 150 · see citing works
Nakkaew, A., Chotigeat, W., Eksomtramage, T., et al. (2008) · Plant Science · doi:10.1016/j.plantsci.2008.05.023 · Cited by 53 · see citing works
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Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae
Maltsev, Y., Kulikovskiy, M., & Maltseva, S. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02244-6 · Cited by 174 · see citing works
“There is evidence of a significant increase in lipid levels during cultivation with nitrogen restriction in Tetradesmus obliquus , Chlorella vulgaris and Mychon-astes homosphaera [34]; in Auxenochlorella pyrenoidosa [35] in Isochrysis galbana [36]; in Microcystis [37].”
Background
- Authors
- Li, Y. X., Zhao, F. J., & Yu, D. D.
- Affiliations
- Qingdao Agricultural University (CN)
- Shandong University of Aeronautics (CN)
- Huangdao Entry-Exit Inspection and Quarantian Bureau, China
- Corresponding author
- Yu, Dong Dong
- Venue
- Brazilian Archives of Biology and Technology
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2015-06
- Volume
- 58
- Issue
- 3
- Pages
- 462-467
- ISSN
- 1516-8913 (ISSN-L)
- OpenAlex
- W1540784925
- Cited by
- 38
- References
- 14
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 7 · 2016: 1
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc
- Locations
- Brazilian Archives of Biology and Technology (publishedVersion, cc-by-nc)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, cc-by-nc)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Qingdao Agricultural University
- Natural Science Foundation of Shandong Province (ZR2011DL012)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Chlorella sorokiniana, nitrogen limitation, lipid accumulation, microalgae, mixotrophic cultivation, cell growth, chlorophyll content, acetyl-CoA carboxylase, gene expression, lipid productivity, stationary phase
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