Multidrug-resistant Candida glabrata strains obtained by induction of anidulafungin resistance in planktonic and biofilm cells
Open accessAbstract
Candida glabrata has emerged as a common cause of serious life-threatening fungal infections, largely owing to their low susceptibility to azole antifungals. Recent guidance indicates the use of echinocandins as the first-choice drug for the treatment of systemic infections of C. glabrata; however, C. glabrata resistance to echinocandins is reportedly increasing. Herein, we present the induction of anidulafungin resistance in planktonic and sessile cells of C. glabrata and the development of fluconazole cross-resistance. MICs of 21 clinical C. glabrata strains were determined by a broth microdilution method using anidulafungin and fluconazole. Biofilm formation on a tracheal catheter was determined using 1- × 1-cm2 polyvinyl polychloride catheter fragments. Induction of anidulafungin resistance in planktonic and sessile cells and evaluation of its stability were performed by exposing the strains to successively higher concentrations of the antifungal. The induction resulted in strains strongly resistant to anidulafungin (MICs: 1-2 µg/mL) and fluconazole (≥64 µg/mL). Most of the sessile cells of C. glabrata presented slightly reduced susceptibility compared with the planktonic cells. Clinically, this cross-resistance could lead to therapeutic failure while using fluconazole in patients previously exposed to subinhibitory concentrations of anidulafungin for extended periods.
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These findings are consistent with a recent report of fluconazole cross-resistance in ani-adapted C. glabrata isolates.
“Importantly, these findings are consistent with a recent report of flz cross-resistance in ani-adapted C. glabrata isolates.38”
From Narrow mutational signatures drive acquisition of multidrug resistance in the fungal pathogen Candida glabrata (2021) · cites this paper as [38]
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- Authors
- Hatwig, C., Balbueno, E. A., Bergamo, V. Z., Pippi, B., Fuentefria, A. M., & Silveira, G. P.
- Affiliations
- Universidade Federal do Rio Grande do Sul (BR)
- Universidade Federal do Rio Grande do Sul, Brazil
- Universidade Federal do Rio Grande do Sul, Brazil; Universidade Federal do Rio Grande do Sul, Brazil
- Venue
- Brazilian Journal of Pharmaceutical Sciences
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2019
- Volume
- 55
- ISSN
- 1984-8250 (ISSN-L)
- 2175-9790
- OpenAlex
- W2996081866
- Cited by
- 6
- References
- 23
- Citations by year
- 2026: 1 · 2024: 3 · 2022: 1 · 2021: 1
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Brazilian Journal of Pharmaceutical Sciences (publishedVersion, cc-by)
- Americanae (AECID Library) (publishedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Lume (Universidade Federal do Rio Grande do Sul) (submittedVersion, cc-by-nc-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (04/2016)
- Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (04/2016)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida glabrata, echinocandin resistance, multidrug resistance, biofilm formation, planktonic cells, antifungal susceptibility, clinical isolates
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