The breakthrough in primary human hepatocytes in vitro expansion
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Primary hepatocytes undergo severe morphological and functional deterioration within 24-48 h in two-dimensional monolayer culture, characterized by loss of polygonal shape, disruption of tight junctions, collapse of bile canaliculi, and gradual downregulation of liver-specific genes.
“PHs undergo severe morphological and functional deterioration within 24–48 h in two-dimensional (2D) monolayer culture, characterized by a loss of polygonal shape, disruption of tight junctions, collapse of bile canaliculi, and gradual downregulation of liver-specific genes [13].”
From The Hepatocyte Expansion Paradox: A Review of In Vitro Challenges and Advances (2026) · cites this paper as [13]
The Introduction says primary human hepatocytes are difficult to culture and expand in vitro, but it does not describe deterioration within 24–48 hours in 2D monolayers. Searches for that timeframe and for the claimed changes in shape, tight junctions, bile canaliculi, and liver-specific genes found no supporting passage in our copy. (LLM, conf=0.72)
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Long-term culture and expansion of primary human hepatocytes
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Romaldini, A., Spanò, R., Catalano, F., Villa, F., Poggi, A. L., & Sabella, S. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.867728 · Cited by 13 · see citing works
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Bhat, S. A., Ahanger, I. A., & Kazim, S. N. (2023) · ACS Omega · doi:10.1021/acsomega.2c07154 · Cited by 6 · see citing works
A breakthrough in liver regeneration for treatment of liver cancer
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The Hepatocyte Expansion Paradox: A Review of In Vitro Challenges and Advances
Kolahdouzmohammadi, M., Tjandra, N., Wu, K., Nikoumaram, R., & Oldani, G. (2026) · Cells · doi:10.3390/cells15161465
“PHs undergo severe morphological and functional deterioration within 24–48 h in two-dimensional (2D) monolayer culture, characterized by a loss of polygonal shape, disruption of tight junctions, collapse of bile canaliculi, and gradual downregulation of liver-specific genes [13].”
Romaldini, A., Spanò, R., & Sabella, S. (2021) · Research Square · doi:10.21203/rs.3.rs-147026/v1
- Authors
- Translational Cancer Research Center, Feng, M., Department of General Surgery, P. U. F. H., Ruirui, K., Translational Cancer Research Center, Yisheng, P., & Translational Cancer Research Center; Department of General Surgery, P. U. F. H.
- Affiliations
- Peking University First Hospital (CN)
- ORCID
- Feng, Mei
- Venue
- Cancer Biology & Medicine
- Publisher
- China Anti-cancer Association
- Published
- 2019-02-01
- Volume
- 16
- Issue
- 1
- Pages
- 1-3
- ISSN
- 2095-3941 (ISSN-L)
- PMC
- PMC6528455
- OpenAlex
- W2939157916
- Cited by
- 7
- References
- 17
- Citations by year
- 2026: 1 · 2023: 2 · 2022: 2 · 2021: 2
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Cancer Biology and Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- primary human hepatocytes, hepatocyte expansion, in vitro culture, hepatocyte culture
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