Gene expression of epigenetic regulatory factors related to primary silencing mechanism is less susceptible to lower doses of bisphenol A in embryonic hypothalamic cells
Open accessAbstract
DNA methyltransferases (DNMTs) are associated with epigenetic regulation of gene expression, and methyl-CpG binding protein 2 (MECP2) acts as a long-range regulator of methylated genes. We evaluated the effects of bisphenol A (BPA) on embryonic mouse hypothalamic cells, with particular emphasis on the gene expression of Dnmts (Dnmt1, Dnmt3a, and Dnmt3b) and Mecp2 isoforms. In a dose-dependent (0.02-200 µM BPA) 3-hr experiment, real-time reverse transcription polymerase chain reaction revealed that gene expression of both Dnmts and Mecp2_e2 was affected at 200 µM and that of Mecp2_e1 was affected at > 20 µM. These results suggest that gene expression of Dnmts and Mecp2 are less susceptible to lower doses of BPA in developing hypothalamic cells. However, as BPA concentration increases, this agent has the potential to alter gene expression of key players that provide stability and flexibility of epigenetic gene regulation, which could disrupt the normal development of hypothalamic functions.
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BPA decreases Dnmt1 and 3a expression but increases Dnmt3b abundance relative to control levels in embryonic hypothalamic mouse cell lines.
“Warita et al . [ 47 ] | BPA | Embryonic hypothalamic mouse cell lines | Decreases Dnmt1 and 3a expression but increases Dnmt3b abundance relative to control levels.”
From DNA methylation: a mechanism linking environmental chemical exposures to risk of autism spectrum disorders? (2016) · cites this paper as [47]
In embryonic hypothalamic mouse cell lines, BPA (200 μM) decreases Dnmt1 and 3a expression by ∼30% but increases Dnmt3b abundance nearly 2-fold relative to control levels.
“In embryonic hypothalamic mouse cell lines, BPA (200 µM) decreases Dnmt1 and 3a expression by ∼30% but increases Dnmt3b abundance nearly 2-fold relative to control levels [ 47 ].”
From DNA methylation: a mechanism linking environmental chemical exposures to risk of autism spectrum disorders? (2016) · cites this paper as [47]
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- Authors
- Warita, K., Mitsuhashi, T., Ohta, K.-I., Suzuki, S., Hoshi, N., Miki, T., & Takeuchi, Y.
- Affiliations
- University of Pittsburgh (US)
- Kagawa University (JP)
- Kobe University (JP)
- Venue
- The Journal of Toxicological Sciences
- Publisher
- Japanese Society of Toxicology
- Published
- 2013
- Volume
- 38
- Issue
- 2
- Pages
- 285-289
- ISSN
- 0388-1350 (ISSN-L)
- 1880-3989
- OpenAlex
- W2030088208
- Cited by
- 41
- References
- 21
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 1 · 2019: 3 · 2018: 6 · 2017: 6 · 2016: 4 · 2015: 7 · 2014: 4 · 2013: 1
- FWCI
- 2.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Toxicological Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (21791037)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- bisphenol A, DNA methyltransferases, DNMT1, DNMT3A, DNMT3B, MeCP2, epigenetic regulation, DNA methylation, dose-dependent effects, endocrine disruptors, hypothalamic development
- MeSH terms
- DNA (Cytosine-5-)-Methyltransferase 1, DNA Methyltransferase 3A, DNA Methyltransferase 3B, Bisphenol A Compounds, Animals, Benzhydryl Compounds, Cells, Cultured, DNA (Cytosine-5-)-Methyltransferases, Dose-Response Relationship, Drug, Hypothalamus, Phenols, RNA, Gene Expression, DNA Methylation, Protein Isoforms, Epigenesis, Genetic, Mice, Methyl-CpG-Binding Protein 2, Real-Time Polymerase Chain Reaction
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