CoChat
Sign Up
Cited by 276

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells

Liang, Gaofeng, Kan, Shu, Zhu, Yanliang, Feng, Shuying, Feng, Wenpo, Gao, Shegan·2018·International Journal of Nanomedicine
DOI 10.2147/ijn.s154458PMC PMC5796471
Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.2147/ijn.s154458

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 Schematic representation showing the design, construction, and transfection of pEGFP-CD63-Apo-A1 in HEK293T cells. Notes: (A) Schematic representations of the engineered Apo-A1 expression vector. (B1–B3) Micrographs of fluorescent, bright field, and merged images of untransfected HEK293T cells. (C1–C3) Micrographs of GFP-fluorescence, bright field, and merged images of pEGFP-CD63-Apo-A1-transfected HEK293T cells. Abbreviation: GFP, green fluorescent protein.
Figure 1 Schematic representation showing the design, construction, and transfection of pEGFP-CD63-Apo-A1 in HEK293T cells. Notes: (A) Schematic representations of the engineered Apo-A1 expression vector. (B1–B3) Micrographs of fluorescent, bright field, and merged images of untransfected HEK293T cells. (C1–C3) Micrographs of GFP-fluorescence, bright field, and merged images of pEGFP-CD63-Apo-A1-transfected HEK293T cells. Abbreviation: GFP, green fluorescent protein.CC BY-NC · PubMed Central
Figure 2 Characterization of exosomes. Notes: (A, B) TEM micrographs of Apo-Exo before and after loading with miR-26a, respectively. (C, D) DLS assay corresponding to (A and B). (E) Western blots of 293T cells untransfected (mock) and transfected with pEGFP-CD63-Apo-A1 vector (transfected). (F) Western blots of exosomes obtained from culture supernatants of 293T cells that had been untreated (mock) and transfected with pEGFP-CD63-Apo-A1 vector (transfected; n=3). Abbreviations: Apo-Exo, Apo-A1-Exosomes; DLS, dynamic light scattering; TEM, transmission electron microscope.
Figure 2 Characterization of exosomes. Notes: (A, B) TEM micrographs of Apo-Exo before and after loading with miR-26a, respectively. (C, D) DLS assay corresponding to (A and B). (E) Western blots of 293T cells untransfected (mock) and transfected with pEGFP-CD63-Apo-A1 vector (transfected). (F) Western blots of exosomes obtained from culture supernatants of 293T cells that had been untreated (mock) and transfected with pEGFP-CD63-Apo-A1 vector (transfected; n=3). Abbreviations: Apo-Exo, Apo-A1-Exosomes; DLS, dynamic light scattering; TEM, transmission electron microscope.CC BY-NC · PubMed Central
Figure 3 Uptake of exosome by HepG2 cells. Notes: (A) Confocal images of HepG2 cells after 12 h incubation with 200 μg/mL of DiO-labeled Exo and Apo-Exo-A1 under 37°C 5% CO2 condition. Images of DiO-labeled exosomes (green) and DAPI (blue) stained nuclei were imaged by merging the confocal images. (B) Relative fluorescence intensity of DiO-labeled exosomes internalized in HepG2 cells. Scale bars represent 100 μm. Data are shown as mean ± SD (n=3) with significance value *p<0.05. Abbreviations: Apo-Exo-A1, Apo-A1-modified exosomes; DiO, 3′-dioctadecyloxacarbocyanine perchlorate.
Figure 3 Uptake of exosome by HepG2 cells. Notes: (A) Confocal images of HepG2 cells after 12 h incubation with 200 μg/mL of DiO-labeled Exo and Apo-Exo-A1 under 37°C 5% CO2 condition. Images of DiO-labeled exosomes (green) and DAPI (blue) stained nuclei were imaged by merging the confocal images. (B) Relative fluorescence intensity of DiO-labeled exosomes internalized in HepG2 cells. Scale bars represent 100 μm. Data are shown as mean ± SD (n=3) with significance value *p<0.05. Abbreviations: Apo-Exo-A1, Apo-A1-modified exosomes; DiO, 3′-dioctadecyloxacarbocyanine perchlorate.CC BY-NC · PubMed Central
Figure 4 Confocal images of Cy5-labeled miR-26a-loaded Apo-Exo in HepG2 cells recorded at different time points. Notes: (A) The uptake of Apo-Exo in different time points (1 h, 3 h, 6 h, 12 h, and 24 h). (B) The relative fluorescence in the Apo-Exo treated HepG2 cells. Scale bar: the first list is 50 μm, and the rest is 10 μm. All data are shown as mean ± SD compared to 1 h group with significance value *p<0.05 (n=3). Abbreviation: Apo-Exo, Apo-A1-Exosomes.
Figure 4 Confocal images of Cy5-labeled miR-26a-loaded Apo-Exo in HepG2 cells recorded at different time points. Notes: (A) The uptake of Apo-Exo in different time points (1 h, 3 h, 6 h, 12 h, and 24 h). (B) The relative fluorescence in the Apo-Exo treated HepG2 cells. Scale bar: the first list is 50 μm, and the rest is 10 μm. All data are shown as mean ± SD compared to 1 h group with significance value *p<0.05 (n=3). Abbreviation: Apo-Exo, Apo-A1-Exosomes.CC BY-NC · PubMed Central
Figure 5 Results of HepG2 cells treated with Apo-Exo/miR-26a in vitro. Notes: (A) Effect of Apo-Exo/miR-26a on HepG2 cells migration. Images were recorded 1, 2, and 4 days after scratching. T1, T2, and T4 represented the first, second, and fourth days. (B) Ratio of migration corresponding to (A). (C) Cell proliferation of HepG2 cells after treatments with different exosome preparations. (D) Relative expression change of miR-26a level in HepG2 cells at different time points (n=3). All data are shown as mean ± SD with significance value *p<0.05. Abbreviation: Apo-Exo, Apo-A1-Exosomes.
Figure 5 Results of HepG2 cells treated with Apo-Exo/miR-26a in vitro. Notes: (A) Effect of Apo-Exo/miR-26a on HepG2 cells migration. Images were recorded 1, 2, and 4 days after scratching. T1, T2, and T4 represented the first, second, and fourth days. (B) Ratio of migration corresponding to (A). (C) Cell proliferation of HepG2 cells after treatments with different exosome preparations. (D) Relative expression change of miR-26a level in HepG2 cells at different time points (n=3). All data are shown as mean ± SD with significance value *p<0.05. Abbreviation: Apo-Exo, Apo-A1-Exosomes.CC BY-NC · PubMed Central
Figure 6 The effects of miR-26a-delivered Apo-Exo on cell cycle and protein expression. Notes: Cell cycle profiles of HepG2 cells after different treatments (A–C) untreated, Exo/miR-26a, and Apo-Exo/miR-26a, respectively. (D) Distribution of the cell cycle untreated and after treated with Exo/miR-26a and Apo-Exo/miR-26a, respectively. (E) Western blot analysis of CCNE2, CCND2, and CDK6 in the HepG2 cells after treatment with CK (untreated), Exo (exo/miR-26a), and Apo-Exo/miR-26a, respectively (n=3). All data are shown as mean ± SD with significance value *p<0.05. Abbreviation: Apo-Exo, Apo-A1-Exosomes.
Figure 6 The effects of miR-26a-delivered Apo-Exo on cell cycle and protein expression. Notes: Cell cycle profiles of HepG2 cells after different treatments (A–C) untreated, Exo/miR-26a, and Apo-Exo/miR-26a, respectively. (D) Distribution of the cell cycle untreated and after treated with Exo/miR-26a and Apo-Exo/miR-26a, respectively. (E) Western blot analysis of CCNE2, CCND2, and CDK6 in the HepG2 cells after treatment with CK (untreated), Exo (exo/miR-26a), and Apo-Exo/miR-26a, respectively (n=3). All data are shown as mean ± SD with significance value *p<0.05. Abbreviation: Apo-Exo, Apo-A1-Exosomes.CC BY-NC · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 3 references

Research works printed but not linked: 46

  1. [1]Niidome T Huang L Gene therapy progress and prospects: nonviral vectors Gene Ther 2002 9 24 1647 1652 12457277
  2. [2]Lee SJ Kim MJ Kwon IC Roberts TM Delivery strategies and potential targets for siRNA in major cancer types Adv Drug Deliv Rev 2016 104 2 15 27259398 PMC4958528
  3. [3]Liang G Li Y Feng W Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells Int J Nanomedicine 2016 11 6079 6088 27895481 PMC5117883
  4. [4]Alvarez-Erviti L Seow Y Yin H Betts C Lakhal S Wood MJ Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes Nat Biotechnol 2011 29 4 341 345 21423189
  5. [5]Dai L Gu N Chen BA Marriott G Human platelets repurposed as vehicles for in vivo imaging of myeloma xenotransplants Oncotarget 2016 7 16 21076 21090 27049725 PMC5008270
  6. [6]Marriott G Engineering platelets for tumour targeting Aging (Albany NY) 2016 8 8 1572 1573 27508429 PMC5032680
  7. [7]Li J Sharkey CC Wun B Liesveld JL King MR Genetic engineering of platelets to neutralize circulating tumor cells J Control Release 2016 228 38 47 26921521 PMC4828270
  8. [8]Shi J Kundrat L Pishesha N Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes Proc Natl Acad Sci U S A 2014 111 28 10131 10136 24982154 PMC4104923
  9. [9]Denzer K Kleijmeer MJ Heijnen HF Stoorvogel W Geuze HJ Exosome: from internal vesicle of the multivesicular body to intercellular signaling device J Cell Sci 2000 113 3365 3374 10984428
  10. [10]Fuhrmann G Herrmann IK Stevens MM Cell-derived vesicles for drug therapy and diagnostics: opportunities and challenges Nano Today 2015 10 3 397 409 28458718 PMC5409525
  11. [11]Eckard SC Rice GI Fabre A The SKIV2L RNA exosome limits activation of the RIG-I-like receptors Nat Immunol 2014 15 9 839 845 25064072 PMC4139417
  12. [12]Gidlof O van der Brug M Ohman J Platelets activated during myocardial infarction release functional miRNA, which can be taken up by endothelial cells and regulate ICAM1 expression Blood 2013 121 9 3908 3917 23493781
  13. [13]Karantalis V Hare JM Use of mesenchymal stem cells for therapy of cardiac disease Circ Res 2015 116 8 1413 1430 25858066 PMC4429294
  14. [14]Powers S Pollack RE Inducing stable reversion to achieve cancer control Nat Rev Cancer 2016 16 4 266 270 27458638
  15. [16]Ghai V Wang K Recent progress toward the use of circulating micro RNAs as clinical biomarkers Arch Toxicol 2016 90 12 2959 2978 27585665
  16. [17]Lee C Carney RP Hazari S 3D plasmonic nanobowl platform for the study of exosomes in solution Nanoscale 2015 7 20 9290 9297 25939587 PMC11781986
  17. [18]Tian Y Li S Song J A doxorubicin delivery platform using engineered natural membrane vesicle exosomes for targeted tumor therapy Biomaterials 2014 35 7 2383 2390 24345736
  18. [19]Kalra H Adda CG Liem M Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma Proteomics 2013 13 22 3354 3364 24115447
  19. [20]Rana S Malinowska K Zoeller M Exosomal tumor microRNA modulates premetastatic organ cells Neoplasia 2013 15 3 281 295 23479506 PMC3593151
  20. [21]Didiot MC Hall LM Coles AH Exosome-mediated delivery of hydrophobically modified siRNA for huntingtin mRNA silencing Mol Ther 2016 24 10 1836 1847 27506293 PMC5112038
  21. [22]Morishita M Takahashi Y Matsumoto A Nishikawa M Takakura Y Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA Biomaterials 2016 111 55 65 27723556
  22. [23]O’Connor RM Gururajan A Dinan TG Kenny PJ Cryan JF All roads lead to the miRNome: miRNAs Have a central role in the molecular pathophysiology of psychiatric disorders Trends Pharmacol Sci 2016 37 12 1029 1044 27832923
  23. [24]Kortylewski M Nechaev S How to train your dragon: targeted delivery of MicroRNA to cancer cells in vivo Mol Ther 2014 22 6 1070 1071 24881764 PMC4048907
  24. [25]Rincon MY VandenDriessche T Chuah MK Gene therapy for cardiovascular disease: advances in vector development, targeting, and delivery for clinical translation Cardiovasc Res 2015 108 1 4 20 26239654 PMC4571836
  25. [26]Hatakeyama H Murata M Sato Y The systemic administration of an anti-miRNA oligonucleotide encapsulated pH-sensitive liposome results in reduced level of hepatic microRNA-122 in mice J Control Release 2014 173 1 43 50 24511611
  26. [27]Scomparin A Polyak D Krivitsky A Satchi-Fainaro R Achieving successful delivery of oligonucleotides – From physico-chemical characterization to in vivo evaluation Biotechnol Adv 2015 33 6 Pt 3 1294 1309 25916823
  27. [28]Kheirolomoom A Kim CW Seo JW Multifunctional nanoparticles facilitate molecular targeting and miRNA delivery to inhibit atherosclerosis in ApoE(−/−) mice Acs Nano 2015 9 9 8885 8897 26308181 PMC4581466
  28. [29]Chen F Huang P Zhu YJ Wu J Zhang CL Cui DX The photoluminescence, drug delivery and imaging properties of multifunctional Eu3+/Gd3+ dual-doped hydroxyapatite nanorods Biomaterials 2011 32 34 9031 9039 21875748
  29. [30]Kota J Chivukula RR O’Donnell KA Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model Cell 2009 137 6 1005 1017 19524505 PMC2722880
  30. [31]Liang G Zhu Y Jing A Cationic microRNA-delivering nanocarriers for efficient treatment of colon carcinoma in xenograft model Gene Ther 2016 23 12 829 838 27482839
  31. [32]Urban S Zieseniss S Werder M Hauser H Budzinski R Engelmann B Scavenger receptor BI transfers major lipoprotein-associated phospholipids into the cells J Biol Chem 2000 275 43 33409 33415 10938082
  32. [33]Yamamoto S Fukuhara T Ono C Lipoprotein receptors redundantly participate in entry of hepatitis C virus PLoS Pathog 2016 12 5 e1005610 27152966 PMC4859476
  33. [34]Kharaziha P Chioureas D Rutishauser D Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel Oncotarget 2015 6 25 21740 21754 25844599 PMC4673300
  34. [35]Lazar I Clement E Ducoux-Petit M Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Pigment Cell Melanoma Res 2015 28 4 464 475 25950383
  35. [37]Hung ME Leonard JN Stabilization of exosome-targeting peptides via engineered glycosylation J Biol Chem 2015 290 13 8166 8172 25657008 PMC4375473
  36. [38]Mo ZC Ren K Liu X Tang ZL Yi GH A high-density lipoprotein-mediated drug delivery system Adv Drug Deliv Rev 2016 106 Pt A 132 147 27208399
  37. [39]Kanada M Bachmann MH Hardy JW Differential fates of biomolecules delivered to target cells via extracellular vesicles Proc Natl Acad Sci U S A 2015 112 12 E1433 E1442 25713383 PMC4378439
  38. [40]Lunavat TR Jang SC Nilsson L RNAi delivery by exosome-mimetic nanovesicles - Implications for targeting c-Myc in cancer Biomaterials 2016 102 231 238 27344366
  39. [41]Chen L Zheng J Zhang Y Tumor-specific expression of MicroRNA-26a suppresses human hepatocellular carcinoma growth via cyclin-dependent and -independent pathways Mol Ther 2011 19 8 1521 1528 21610700 PMC3149175
  40. [42]Gandhi NS Tekade RK Chougule MB Nanocarrier mediated delivery of siRNA/miRNA in combination with chemotherapeutic agents for cancer therapy: current progress and advances J Control Release 2014 194 238 256 25204288 PMC4254052
  41. [43]Tkach M Thery C Communication by extracellular vesicles: where we are and where we need to go Cell 2016 164 6 1226 1232 26967288
  42. [45]Choi JS Yoon HI Lee KS Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration J Control Release 2016 222 107 115 26699421
  43. [46]Tian Y Li S Song J A doxorubicin delivery platform using engineered natural membrane vesicle exosomes for targeted tumor therapy Biomaterials 2014 35 7 2383 2390 24345736
  44. [47]Martinez LO Jacquet S Esteve JP Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis Nature 2003 421 6918 75 79 12511957
  45. [48]Tominaga N Yoshioka Y Ochiya T A novel platform for cancer therapy using extracellular vesicles Adv Drug Deliv Rev 2015 95 50 55 26482189
  46. [49]Jo W Kim J Yoon J Large-scale generation of cell-derived nanovesicles Nanoscale 2014 6 20 12056 12064 25189198

Cited by

Sorted by citations

See all 276

Metadata

Authors
Liang, G., Kan, S., Zhu, Y., Feng, S., Feng, W., & Gao, S.
Venue
International Journal of Nanomedicine
Publisher
Informa UK Limited
Published
2018-01
Volume
Volume 13
Pages
585-599
ISSN
1178-2013
DOI
10.2147/ijn.s154458
PMC
PMC5796471
Cited by
276
References
3
Language
en
Licence
creativecommons.org/licenses/by-nc/3.0/ (unspecified)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells. https://app.cochat.ai/papers/10.2147/ijn.s154458
The paper
Liang, G., Kan, S., Zhu, Y., Feng, S., Feng, W., & Gao, S. (2018). Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells. International Journal of Nanomedicine. https://doi.org/10.2147/ijn.s154458