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Cited by 428Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryNanoplatforms for cancer theranostics

Engineered Extracellular Vesicles for Cancer Therapy

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Zhang, Xu, Zhang, Hongbo, Gu, Jianmei, Zhang, Jiayin, Shi, Hui, Qian, Hui, Wang, Dongqing, Xu, Wenrong +2 more·2021·Advanced Materials
DOI 10.1002/adma.202005709
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Abstract

Extracellular vesicles (EVs) have emerged as a novel cell‐free strategy for the treatment of many diseases including cancer. As a result of their natural properties to mediate cell‐to‐cell communication and their high physiochemical stability and biocompatibility, EVs are considered as excellent delivery vehicles for a variety of therapeutic agents such as nucleic acids and proteins, drugs, and nanomaterials. Increasing studies have shown that EVs can be modified, engineered, or designed to improve their efficiency, specificity, and safety for cancer therapy. Herein, a comprehensive overview of the recent advances in the strategies and methodologies of engineering EVs for scalable production and improved cargo‐loading and tumor‐targeting is provided. Additionally, the potential applications of engineered EVs in cancer therapy are discussed by presenting prominent examples, and the opportunities and challenges for translating engineered EVs into clinical practice are evaluated.

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  • Other mechanical methods rely on disrupting the EV membrane for cargo loading, including freeze-thaw cycles, sonication, and extrusion.

    “Other mechanical methods also rely on disrupting the EV membrane for cargo loading, including freeze-thaw cycles, sonication, and extrusion [222].”

    From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [222]

    Section 4.1.2 (page not available) identifies freeze–thaw cycles, sonication, and extrusion as mechanical methods for loading cargo into EVs. It says mechanical force may compromise EV membrane integrity, but does not establish membrane disruption as the mechanism these methods rely on. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • The cargo-carrying capacity and selectivity of exosomes can be improved for greater therapeutic effects using various modified exosomal production technologies through chemical or genetic engineering.

    “The cargo-carrying capacity and selectivity of exosomes can be improved for greater therapeutic effects using various modified exosomal production technologies through chemical or genetic engineering.”

    From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [18]

  • Exosomes can be engineered to target specific cells or tissues, which will enhance the efficacy and reduce the side effects of mRNAs.

    “Moreover, these exosomes can be engineered to utilize the natural carriers to target specific cells or tissues. This targeted approach will enhance the efficacy and reduce the side effects of mRNAs.”

    From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [18]

  • Engineered exosomes can deliver mRNA to specific sites, avoid immune system detection and early degradation, overcome biological barriers, and even control the release of the drug in response to a specific external stimulus.

    “Engineered exosomes can deliver mRNA to specific sites, avoid immune system detection and early degradation, overcome biological barriers, and even control the release of the drug in response to a specific external stimulus [18, 19].”

    From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [18]

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Authors
Zhang, X., Zhang, H., Gu, J., Zhang, J., Shi, H., Qian, H., Wang, D., Xu, W., Pan, J., & Santos, H. A.
Affiliations
  • Jiangsu University (CN)
  • University of Helsinki (FI)
Corresponding author
  • Zhang, Xu
  • Pan, Jianming
  • Santos, Hélder A.
ORCID
Venue
Advanced Materials
Publisher
Wiley
Published
2021-02-28
Volume
33
Issue
14
Pages
e2005709-e2005709
ISSN
  • 0935-9648 (ISSN-L)
  • 1521-4095
DOI
10.1002/adma.202005709
OpenAlex
W3096517545
Cited by
428
References
175
Citations by year
2026: 41 · 2025: 113 · 2024: 97 · 2023: 96 · 2022: 56 · 2021: 24 · 2013: 1
FWCI
7.15
Type
journal article
Language
en
Open access
Licence
Locations
  • Advanced Materials (publishedVersion)
  • PubMed (publishedVersion)
  • Työväentutkimus Vuosikirja (submittedVersion, cc-by)
  • Åbo Akademi University Research Portal (submittedVersion, other-oa)
Funders
  • National Natural Science Foundation of China (81972310, 81672416, 22078132, 21822807)
  • Priority Academic Program Development of Jiangsu Higher Education Institutions
  • Jiangsu Provincial Key Research and Development Program (BE2018690)
  • Natural Science Foundation of Jiangsu Province (18KJA320001)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
Keywords
extracellular vesicles, engineered extracellular vesicles, cancer therapy, cell-free therapy, drug carriers, cargo loading, tumor targeting, scalable fabrication, nucleic acid delivery, clinical translation
MeSH terms
Extracellular Vesicles, Animals, Antineoplastic Agents, Drug Carriers, Humans, Neoplasms, Drug Delivery Systems

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