Cited by 317Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryNanoplatforms for cancer theranostics

A Nanodrug Consisting Of Doxorubicin And Exosome Derived From Mesenchymal Stem Cells For Osteosarcoma Treatment In Vitro

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Wei, Hongxiang, Chen, Jinyuan, Wang, Shenglin, Fu, Feihuan, Zhu, Xia, Wu, Chaoyang, Liu, Zhoujie, Zhong, Guangxian +1 more·2019·International Journal of Nanomedicine

Abstract

PURPOSE: The primary goal of the present study was to develop the nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro. METHODS: The exosomes were isolated from bone marrow MSCs (BM-MSCs) by an Exosome Isolation Kit. The exosome-loaded doxorubicin (Exo-Dox) was prepared by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. The nanoparticle tracking analysis (NTA) and transmission electron microscope (TEM) were used to characterize of the exosome and Exo-Dox. The cytotoxicity of Exo-Dox was determined by CCK-8 assay. Further, the cellular uptake of different drugs was analyzed using inverted fluorescence microscope and flow cytometry. RESULTS: The typical exosome structures can be observed by TEM. After loading with doxorubicin, its size is larger than free exosome. Compared with the free Dox, the prepared Exo-Dox showed enhanced cellular uptake efficiency and anti-tumor effect in osteosarcoma MG63 cell line but low cytotoxicity in myocardial H9C2 cell line. CONCLUSION: The prepared Exo-Dox could be used as an excellent chemotherapeutic drug for treatment of osteosarcoma in vitro. Considering the tumor-homing feature of BM-MSCs, the Exo-Dox may be a good candidate for targeted osteosarcoma treatment in future study.

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Authors
Wei, H., Chen, J., Wang, S., Fu, F., Zhu, X., Wu, C., Liu, Z., Zhong, G., & Lin, J.
Affiliations
First Affiliated Hospital of Fujian Medical University (CN)
Venue
International Journal of Nanomedicine
Published
2019-11
Volume
Volume 14
Pages
8603-8610
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W2982334421
Cited by
317
References
34
Citations by year
2026: 30 · 2025: 44 · 2024: 53 · 2023: 76 · 2022: 56 · 2021: 38 · 2020: 20
FWCI
9.46
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
Keywords
doxorubicin, exosomes, mesenchymal stem cells, osteosarcoma, nanotherapeutics, drug delivery, bone marrow mesenchymal stem cells, cellular uptake, cytotoxicity, targeted chemotherapy, MG-63 cells, H9c2 cells
MeSH terms
Animals, Antineoplastic Agents, Bone Neoplasms, Cell Survival, Doxorubicin, Humans, Osteosarcoma, Cell Death, Cell Line, Tumor, Mice, Rats, Nanoparticles, Exosomes, Mesenchymal Stem Cells, Drug Liberation

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