A Nanodrug Consisting Of Doxorubicin And Exosome Derived From Mesenchymal Stem Cells For Osteosarcoma Treatment In Vitro
Open accessAbstract
PURPOSE: The primary goal of the present study was to develop the nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro. METHODS: The exosomes were isolated from bone marrow MSCs (BM-MSCs) by an Exosome Isolation Kit. The exosome-loaded doxorubicin (Exo-Dox) was prepared by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. The nanoparticle tracking analysis (NTA) and transmission electron microscope (TEM) were used to characterize of the exosome and Exo-Dox. The cytotoxicity of Exo-Dox was determined by CCK-8 assay. Further, the cellular uptake of different drugs was analyzed using inverted fluorescence microscope and flow cytometry. RESULTS: The typical exosome structures can be observed by TEM. After loading with doxorubicin, its size is larger than free exosome. Compared with the free Dox, the prepared Exo-Dox showed enhanced cellular uptake efficiency and anti-tumor effect in osteosarcoma MG63 cell line but low cytotoxicity in myocardial H9C2 cell line. CONCLUSION: The prepared Exo-Dox could be used as an excellent chemotherapeutic drug for treatment of osteosarcoma in vitro. Considering the tumor-homing feature of BM-MSCs, the Exo-Dox may be a good candidate for targeted osteosarcoma treatment in future study.
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Engineered exosomes that silenced Bcl-2 and Bax had a therapeutic effect on apoptosis after traumatic brain injury.
“Wang et al. studied engineered exosomes that silenced Bcl-2 and Bax with a promising result that suggested the exosomes had a therapeutic effect on apoptosis after TBI [72].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [72]
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- Authors
- Wei, H., Chen, J., Wang, S., Fu, F., Zhu, X., Wu, C., Liu, Z., Zhong, G., & Lin, J.
- Affiliations
- First Affiliated Hospital of Fujian Medical University (CN)
- Venue
- International Journal of Nanomedicine
- Publisher
- Informa UK Limited
- Published
- 2019-11
- Volume
- Volume 14
- Pages
- 8603-8610
- ISSN
- 1176-9114 (ISSN-L)
- 1178-2013
- PMC
- PMC6830377
- OpenAlex
- W2982334421
- Cited by
- 317
- References
- 34
- Citations by year
- 2026: 30 · 2025: 44 · 2024: 53 · 2023: 76 · 2022: 56 · 2021: 38 · 2020: 20
- FWCI
- 9.46
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Nanomedicine (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- doxorubicin, exosomes, mesenchymal stem cells, osteosarcoma, nanotherapeutics, drug delivery, bone marrow mesenchymal stem cells, cellular uptake, cytotoxicity, targeted chemotherapy, MG-63 cells, H9c2 cells
- MeSH terms
- Animals, Antineoplastic Agents, Bone Neoplasms, Cell Survival, Doxorubicin, Humans, Osteosarcoma, Cell Death, Cell Line, Tumor, Mice, Rats, Nanoparticles, Exosomes, Mesenchymal Stem Cells, Drug Liberation
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