Phytoecdysteroids effects on mammalians, isolation and analysis
Abstract
Ecdysteroids are known insect moulting hormones, regulating the insects' metamorphosis. At the same time, ecdysteroids reveal beneficial effects on humans and animals alike. Medicinal plants have been subjected to an intensive research, addressing the presence of ecdysteroids. The possible utilization of medicinal plant deals with their use as raw materials for health preparations and also for the isolation of new phytoecdysteroids. Research on the plant ecdysteroids involves two basic lines. Isolation of major compounds and scout their physiological effects; and isolation of minor ecdysteroids to find new compounds. This review summarizes the recent efforts in the ecdysteroid research including their indication as health improvement preparations, chromatography of ecdysteroids and certain methods for identification of new ecdysteroids.
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Beta-ecdysone has no direct binding to the androgen receptor or other cytosolic steroid receptors.
“However, βEcd is shown to have no direct binding to the androgen receptor or other cytosolic steroid receptors [25, 64, 65]”
From Prevention of glucocorticoid induced bone changes with beta-ecdysone (2015) · cites this paper as [64]
The anabolic effect of beta-ecdysone on male skeleton may be independent of its androgen-like structure.
“suggesting that our observation of an anabolic effect of βEcd on male skeleton may be independent of its androgen-like structure.”
From Prevention of glucocorticoid induced bone changes with beta-ecdysone (2015) · cites this paper as [64]
Cited by
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Innovations in Health Value and Functional Food Development of Quinoa ( Chenopodium quinoa Willd.)
Graf, B. L., Rojas‐Silva, P., Rojo, L. E., Delatorre-Herrera, J., Baldeon, M. E., & Raskin, I. (2015) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12135 · Cited by 290 · see citing works
“Extracts from several phytoecdysteroid-containing plants, especially traditional medicinal Chinese and Siberian herbs (Cheng and others 2008; Kokoska and Janovska 2009; Lafont 1998), have been used as adaptogens, muscle builders, and stress reducers (Báthori 2002).”
MethodEffects and applications of arthropod steroid hormones (ecdysteroids) in mammals
Dinan, L., & Lafont, R. (2006) · Journal of Endocrinology · doi:10.1677/joe.1.06900 · Cited by 200 · see citing works
“This was the starting point for many experiments, which reported a wide array of pharmacological effects of ecdysteroids on mammals (Sláma & Lafont 1995, Syrov 2000, Kholodova 2001, Báthori 2002, Lafont & Dinan 2003, Klein 2004, Báthori & Pongrácz 2005).”
Practical uses for ecdysteroids in mammals including humans: and update
Lafont, R., & Dinan, L. (2003) · Journal of Insect Science · doi:10.1093/jis/3.1.7 · Cited by 196 · see citing works
“…effects of ecdysteroids on vertebrates The pharmacological actions of ecdysteroids on vertebrates have been reviewed in several previous articles (Burdette, 1962, 1972; Ogawa et al., 1974; Syrov, 1984, 1994; Sláma and Lafont, 1995; Xu et al., 1997; Syrov, 2000; Kholodova, 2001; Báthori, 2002).”
BackgroundPractical uses for ecdysteroids in mammals including humans: And update
Lafont, R., & Dinan, L. (2003) · Journal of Insect Science · doi:10.1673/031.003.0701 · Cited by 196 · see citing works
“…effects of ecdysteroids on vertebrates The pharmacological actions of ecdysteroids on vertebrates have been reviewed in several previous articles (Burdette, 1962, 1972; Ogawa et al., 1974; Syrov, 1984, 1994; Sláma and Lafont, 1995; Xu et al., 1997; Syrov, 2000; Kholodova, 2001; Báthori, 2002).”
Lokhande, V. H., Gor, B. K., Desai, N. S., Nikam, T. D., & Suprasanna, P. (2012) · Agronomy for Sustainable Development · doi:10.1007/s13593-012-0113-x · Cited by 134 · see citing works
“…application, 20E and derivatives are used for health improvement as they have been shown to stimulate the synthesis of proteins, builds muscle, be adaptogenic for human immunodeficiency virus (HIV) patients and have antioxidant and tonic properties (Bathori 2002; Sinlaparaya et al. 2007).”
MethodQuinoa seeds leach phytoecdysteroids and other compounds with anti-diabetic properties
Graf, B. L., Poulev, A., Kuhn, P., Grace, M. H., Lila, M. A., & Raskin, I. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.04.088 · Cited by 129 · see citing works
“Phytoecdysteroids, extracted from these botanical sources, have been marketed and sold in commercially available health products, such as adaptogens, body building agents, stress reducers, performance enhancers and cosmetics (Bathori, 2002).”
BackgroundDinan, L., Dioh, W., Veillet, S., & Lafont, R. (2021) · Biomedicines · doi:10.3390/biomedicines9050492 · Cited by 99 · see citing works
“This topic has been extensively reviewed previously [7,33–45], so here we shall just present some data from more recent studies on the anabolic and hypolipidemic effects by way of example.”
Kayani, W. K., Dilshad, E., Ahmed, T., Ismail, H., & Mirza, B. (2016) · BMC Complementary and Alternative Medicine · doi:10.1186/s12906-016-1363-y · Cited by 85 · see citing works
Ecdysteroids from the Antarctic Tunicate Synoicum adareanum
Miyata, Y., Diyabalanage, T. K. K., Amsler, C. D., McClintock, J. B., Valeriote, F. A., & Baker, B. J. (2007) · Journal of Natural Products · doi:10.1021/np0702739 · Cited by 74 · see citing works
Muchate, N. S., Rajurkar, N. S., Suprasanna, P., & Nikam, T. D. (2019) · Scientific Reports · doi:10.1038/s41598-019-48737-6 · Cited by 66 · see citing works
Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
Kumpun, S., Girault, J.-P., Dinan, L., Blais, C., Maria, A., Dauphin-Villemant, C., Yingyongnarongkul, B., Suksamrarn, A., & Lafont, R. (2011) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2011.03.016 · Cited by 45 · see citing works
Prevention of glucocorticoid induced bone changes with beta-ecdysone
Dai, W., Jiang, L., Lay, Y.-A. E., Chen, H., Jin, G., Zhang, H., Kot, A., Ritchie, R. O., Lane, N. E., & Yao, W. (2015) · Bone · doi:10.1016/j.bone.2015.01.001 · Cited by 30 · see citing works
“However, βEcd is shown to have no direct binding to the androgen receptor or other cytosolic steroid receptors [25,64,65], suggesting that our observation of an anabolic effect of βEcd on male skeleton may be independent of its androgen-like structure.”
Role of phytoecdysteroids in insect pest management: a review
Chaubey, M. K. (2017) · Journal of Agronomy · doi:10.3923/ja.2018.1.10 · Cited by 30 · see citing works
Ecdysterone and its Activity on some Degenerative Diseases
Cahlíková, L., Macáková, K., Chlebek, J., Hošťálková, A., Kulhánková, A., & Opletal, L. (2011) · Natural Product Communications · doi:10.1177/1934578x1100600527 · Cited by 26 · see citing works
“Extraction with organic solvents is used for the isolation and purification of this type of substance [34].”
MethodMamadalieva, N. Z. (2012) · Redalyc (Universidad Autónoma del Estado de México) · Cited by 26 · see citing works
Ishola, I. O., Ochieng, C. O., Olayemi, S. O., Jimoh, M. O., & Lawal, S. M. (2014) · Pharmacology Biochemistry and Behavior · doi:10.1016/j.pbb.2014.11.005 · Cited by 22 · see citing works
“In addition, they exert protective, preventive, corrective, and adaptogenic effects (Aikake et al., 1996; Najmutdinova and Saatov, 1999; Bathori, 2002).”
Innovative and Future Applications for Ecdysteroids
Lafont, R., & Dinan, L. N. (2009) · Springer eBooks · doi:10.1007/978-1-4020-9112-4_23 · Cited by 17 · see citing works
“On the other hand, many plants used for traditional medicine belong to ecdysteroidrich species (Slama and Lafont, 1995; Lafont, 1998; Bathori, 2002 ).”
Backgroundβ-Ecdysone Augments Peak Bone Mass in Mice of Both Sexes
Dai, W., Zhang, H., Zhong, Z. A., Jiang, L., Chen, H., Lay, Y.-A. E., Kot, A., Ritchie, R. O., Lane, N. E., & Yao, W. (2015) · Clinical Orthopaedics & Related Research · doi:10.1007/s11999-015-4246-5 · Cited by 15 · see citing works
Lipophilicity Determination using Both TLC and Calculations
Kalász, H., Benkő, B., Gulyás, Z., et al. (2009) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826070902854466 · Cited by 15 · see citing works
“Details on isolation and the most important physico-chemical characteristics of ecdysteroids were detailed in her earlier publications.([23,24,25]) Calculation of logP and total polar surface area (TPSA) values were done using the Prolog Program of Pallas software of CompuDrug International, Inc.”
MethodPhytoecdysteroids: A Novel, Non-Androgenic Alternative for Muscle Health and Performance
McBride, J. M. (2013) · Journal of Steroids & Hormonal Science · doi:10.4172/2157-7536.s12-e001 · Cited by 14 · see citing works
Sinlaparaya, D., Duanghaklang, P., & Panichajakul, S. (2007) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.4314/ajb.v6i14.57734 · Cited by 14 · see citing works
“20-hydroxyecdysone and derivatives were prepared for health improvement; they have been shown to stimulate the synthesis of protein, builds muscle, be adaptogenic for HIV patients, have antioxidant and tonics properties (Bathori, 2002).”
Low-polarity phytoecdysteroids from the juice of Serratula coronata L. (Asteraceae)
Odinokov, V. N., Kumpun, S., Galyautdinov, I. V., Evrard-Todeschi, N., Veskina, N. A., Khalilov, L. M., Girault, J.-P., Dinan, L., Maria, A., & Lafont, R. (2005) · Collection of Czechoslovak Chemical Communications · doi:10.1135/cccc20052038 · Cited by 23 · see citing works
Urinary Excretion of Ecdysterone and Its Metabolites Following Spinach Consumption
Yuliandra, T., Touvleliou, K., de la Torre, X., Botrè, F., Loke, S., Isenmann, E., Valder, S., Diel, P., & Parr, M. K. (2023) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202200518 · Cited by 12 · see citing works
Extraction and Monitoring of Phytoecdysteroids Through HPLC
Ghosh, D., & Laddha, K. S. (2006) · Journal of Chromatographic Science · doi:10.1093/chromsci/44.1.22 · Cited by 11 · see citing works
TLC Determination of Hydrophilicity Parameter of Some Pyridinium Aldoximes
Laufer, R., Báthori, M., Csermely, T., et al. (2007) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826070701451738 · Cited by 10 · see citing works
Self-citationChamnipa, J. (2012) · Journal of Medicinal Plants Research · doi:10.5897/jmpr12.059 · Cited by 10 · see citing works
“as insecticides (Dhadialla and Tzertzinis, 1998), a growth stimulating agent for shrimp culture (Chaiwatcharakool, 1986), an anabolic steroid in sport and bodybuilding and tonic supplement for male and female reproductive system (Ahmad et al., 2000c; Bathori, 2002; Dinan and Lafont, 2006).”
BackgroundParis, A., André, F., Antignac, J., et al. (2006) · INRAE Productions Animales · doi:10.20870/productions-animales.2006.19.3.3491 · Cited by 10 · see citing works
“Slama K., Lafont R., 1995.”
Wu, J., Cheng, K., Wang, H., Ye, W., Li, E. T. S., & Wang, M. (2008) · Phytochemical Analysis · doi:10.1002/pca.1097 · Cited by 9 · see citing works
Mokua, G. N. (2013) · Kenyatta University Institutional Repository (Kenyatta University) · doi:10.12691/wjoc-1-1-1 · Cited by 8 · see citing works
“They affect a wide range of insects at low concentrations and are not harmful to human and animal cells [10].”
BackgroundInduced accumulation of 20-hydroxyecdysone in cell suspension cultures of Vitex glabrata R.Br.
Thanonkeo, P. (2011) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb11.1543 · Cited by 7 · see citing works
“…example, as a growth stimulating agent for shrimp culture (Chaiwatcharakool, 1986), insecticides (Dhadialla and Tzertzinis, 1998), an anabolic steroid in sport and bodybuilding, and tonic supplement for male and female reproductive system (Ahmad et al., 2000; Bathori, 2002; Dinan and Lafont, 2006).”
BackgroundNovel Results of Two‐Dimensional Thin‐Layer Chromatography
Kalász, H., Hunyadi, A., & Báthori, M. (2005) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826070500189612 · Cited by 6 · see citing works
“The roots of Serratula wolffii were extracted in the usual way.([5]) The extract of Silene viridifloras was used without any preliminary purification.”
MethodSelf-citationThin‐Layer Chromatography of Phytoecdysteroids
Báthori, M., Kalász, H., Janicsák, G., Pongrácz, Z., & Vámos, J. (2003) · Journal of Liquid Chromatography & Related Technologies · doi:10.1081/jlc-120024534 · Cited by 6 · see citing works
Self-citationBiological activity of phytosterols and their derivatives
Mehtiev, A. R., & Misharin, A. Y. (2008) · Biochemistry (Moscow) Supplement Series B Biomedical Chemistry · doi:10.1007/s11828-008-1001-4 · Cited by 4 · see citing works
Spot and Mobile-Phase Front Anomalies in Planar (Thin-Layer) Chromatography
Kalász, H. (2005) · Chromatographia · doi:10.1365/s10337-005-0605-9 · Cited by 3 · see citing works
de Castro, S. C., Oliveira, N. M., de Oliveira, J. R., Machado, L. L., Leão, K. V., & Bueno, M. A. (2021) · Revista Virtual de Química · doi:10.21577/1984-6835.20210075 · Cited by 2 · see citing works
“Several studies have demonstrated the biological properties of 20E 21,22,23 , including analgesic activity.”
Biological Activities and Phytochemistry of Sesuvium portulacastrum (L.) L.
Shelke, D. B., Chambhare, M. R., Nikam, T. D., & Suprasanna, P. (2025) · Reference series in phytochemistry · doi:10.1007/978-3-031-63920-3_15-1 · Cited by 2 · see citing works
Valorization of Secondary Metabolites in Plants
Patil-Patankar, V., Yadav‐Bhagwat, P., & Kedari, P. (2022) · Wiley eBooks · doi:10.1002/9781119818816.ch13 · Cited by 2 · see citing works
Prieto, J. M., Barker, A. M., Schaffner, U., et al. (2024) · Plants · doi:10.3390/plants13162230 · Cited by 1 · see citing works
“The identification of individual constituents was achieved by matching mass spectral data with those held in the NIST98 [46] and a home-made library mass spectra from pure substances.”
MethodEcdiesteroides y su actividad farmacológica sobre el músculo esquelético
Egocheaga, V. O. (2023) · Ars Pharmaceutica (Internet) · doi:10.30827/ars.v64i4.28863
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Biological Activities and Phytochemistry of Sesuvium portulacastrum (L.) L.
Shelke, D. B., Chambhare, M. R., Nikam, T. D., & Suprasanna, P. (2025) · Reference series in phytochemistry · doi:10.1007/978-3-031-91066-1_15
- Authors
- Bathori, M.
- Venue
- Mini-Reviews in Medicinal Chemistry
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2002-06-01
- Volume
- 2
- Issue
- 3
- Pages
- 285-293
- ISSN
- 1389-5575 (ISSN-L)
- OpenAlex
- W2024140170
- Cited by
- 42
- References
- 0
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2019: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mini-Reviews in Medicinal Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hungarian Scientific Research Fund
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- phytoecdysteroids, ecdysteroids, medicinal plants, chromatographic separation
- MeSH terms
- Animals, Ecdysone, Humans, Mammals, Phytotherapy, Phytosterols, Plant Extracts, Plants, Medicinal, Structure-Activity Relationship, Dietary Supplements, Ecdysteroids
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