Subchronic Administration of Phencyclidine Produces Hypermethylation in the Parvalbumin Gene Promoter in Rat Brain
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AIM: A deficit in parvalbumin neurons is found in schizophrenia and several animal models of the disease. In this preliminary study, we determined whether one such model, phencyclidine (PCP) administration, results in changes in DNA methylation in the rat Pvalb promoter. MATERIALS & METHODS: DNA from hippocampus and prefrontal cortex from rats, which 6 weeks previously received either 2 mg/kg PCP or vehicle for 7 days, underwent bisulphite pyrosequencing to determine methylation. RESULTS: PCP administration induced significantly greater methylation at one of two Pvalb CpG sites in both prefrontal cortex and hippocampus, while no significant difference was found in long interspersed nucleotide element-1, a global measure of DNA methylation. CONCLUSION: Subchronic PCP administration results in a specific hypermethylation in the Pvalb promoter which may contribute to parvalbumin deficits in this animal model of psychosis.
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- Authors
- Fachim, H. A., Srisawat, U., Dalton, C. F., Harte, M. K., Marsh, S., Neill, J. C., & Reynolds, G. P.
- Affiliations
- Sheffield Hallam University (GB)
- University of Manchester (GB)
- ORCID
- Venue
- Epigenomics
- Publisher
- Informa UK Limited
- Published
- 2016-08-16
- Volume
- 8
- Issue
- 9
- Pages
- 1179-1183
- ISSN
- 1750-1911 (ISSN-L)
- 1750-192X
- OpenAlex
- W2516037232
- Cited by
- 22
- References
- 29
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 1
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- Licence
- other-oa
- Locations
- Epigenomics (publishedVersion)
- PubMed (publishedVersion)
- SHURA (Sheffield Hallam University Research Archive) (Sheffield Hallam University) (acceptedVersion)
- Research Explorer (The University of Manchester) (submittedVersion, other-oa)
- Funders
- FAPESP (2015/02948-7)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Health Sciences › Medicine › Psychiatry and Mental health › Schizophrenia research and treatment
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- phencyclidine, subchronic administration, DNA methylation, promoter hypermethylation, parvalbumin, schizophrenia, hippocampus, prefrontal cortex, CpG sites
- MeSH terms
- Animals, Brain, Female, Parvalbumins, Phencyclidine, Promoter Regions, Genetic, Excitatory Amino Acid Antagonists, CpG Islands, DNA Methylation, Long Interspersed Nucleotide Elements, Rats
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