Cited by 22Epigenetics and DNA MethylationSchizophrenia research and treatmentNeurotransmitter Receptor Influence on Behavior

Subchronic Administration of Phencyclidine Produces Hypermethylation in the Parvalbumin Gene Promoter in Rat Brain

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Fachim, Helene A, Srisawat, Umarat, Dalton, Caroline F, Harte, Michael K, Marsh, Samuel, Neill, Joanna C, Reynolds, Gavin P·2016·Epigenomics

Abstract

AIM: A deficit in parvalbumin neurons is found in schizophrenia and several animal models of the disease. In this preliminary study, we determined whether one such model, phencyclidine (PCP) administration, results in changes in DNA methylation in the rat Pvalb promoter. MATERIALS & METHODS: DNA from hippocampus and prefrontal cortex from rats, which 6 weeks previously received either 2 mg/kg PCP or vehicle for 7 days, underwent bisulphite pyrosequencing to determine methylation. RESULTS: PCP administration induced significantly greater methylation at one of two Pvalb CpG sites in both prefrontal cortex and hippocampus, while no significant difference was found in long interspersed nucleotide element-1, a global measure of DNA methylation. CONCLUSION: Subchronic PCP administration results in a specific hypermethylation in the Pvalb promoter which may contribute to parvalbumin deficits in this animal model of psychosis.

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Crossref · 10.2217/epi-2016-0050

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Authors
Fachim, H. A., Srisawat, U., Dalton, C. F., Harte, M. K., Marsh, S., Neill, J. C., & Reynolds, G. P.
Affiliations
  • Sheffield Hallam University (GB)
  • University of Manchester (GB)
Venue
Epigenomics
Published
2016-08-16
Volume
8
Issue
9
Pages
1179-1183
ISSN
  • 1750-1911 (ISSN-L)
  • 1750-192X
OpenAlex
W2516037232
Cited by
22
References
29
Citations by year
2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 1
FWCI
1.91
Type
journal article
Language
en
Licence
other-oa
Locations
  • Epigenomics (publishedVersion)
  • PubMed (publishedVersion)
  • SHURA (Sheffield Hallam University Research Archive) (Sheffield Hallam University) (acceptedVersion)
  • Research Explorer (The University of Manchester) (submittedVersion, other-oa)
Funders
FAPESP (2015/02948-7)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Health Sciences › Medicine › Psychiatry and Mental health › Schizophrenia research and treatment
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
phencyclidine, subchronic administration, DNA methylation, promoter hypermethylation, parvalbumin, schizophrenia, hippocampus, prefrontal cortex, CpG sites
MeSH terms
Animals, Brain, Female, Parvalbumins, Phencyclidine, Promoter Regions, Genetic, Excitatory Amino Acid Antagonists, CpG Islands, DNA Methylation, Long Interspersed Nucleotide Elements, Rats

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