The apical sensory organ of a gastropod veliger is a receptor for settlement cues
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On the basis of anatomy and larval behavior, the apical sensory organ (ASO) of gastropod veliger larvae has been implicated as the site of perception of cues for settlement and metamorphosis. Until now, there have been no experimental data to support this hypothesis. In this study, cells in the ASO of veliger larvae of the tropical nudibranch Phestilla sibogae were stained with the styryl vital dye DASPEI and then irradiated with a narrow excitatory light beam on a fluorescence microscope. When its ASO cells were bleached by irradiation for 20 min or longer, an otherwise healthy larva was no longer able to respond to the usual metamorphic cue, a soluble metabolite from a coral prey of the adult nudibranch. The irradiated cells absorbed the dye acridine orange, suggesting that they were dying. When larvae not stained with DASPEI were similarly irradiated, or when stained larvae were irradiated with the light beam focused on other parts of the body, there was no loss of ability to metamorphose. Together these data provide strong support for the hypothesis. Potassium and cesium ions, known to induce metamorphosis in larvae of many marine-invertebrate phyla, continue to induce metamorphosis in larvae that have lost the ability to respond to the coral inducer due to staining and irradiation. These results demonstrate that (1) the ASO-ablated larvae have not lost the ability to metamorphose and (2) the ions do not act only on the metamorphic-signal receptor cells, but at other sites downstream in the metamorphic signal transduction pathway.
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This environmental-cue-triggered metamorphosis pattern is seen across corals, annelids, molluscs, ascidians and others.
“This pattern is seen across corals, annelids, molluscs, ascidians and others [73-77].”
From An option space for early neural evolution (2015) · cites this paper as [75]
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- Authors
- Hadfield, M., Meleshkevitch, E., & Boudko, D.
- Affiliations
- University of Hawaiʻi at Mānoa (US)
- Venue
- The Biological Bulletin
- Publisher
- University of Chicago Press
- Published
- 2000-02
- Volume
- 198
- Issue
- 1
- Pages
- 67-76
- ISSN
- 0006-3185 (ISSN-L)
- 1939-8697
- OpenAlex
- W2102241613
- Cited by
- 176
- References
- 58
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 6 · 2022: 3 · 2021: 7 · 2020: 15 · 2019: 4 · 2018: 5 · 2017: 5 · 2016: 6 · 2015: 9 · 2014: 6 · 2013: 6 · 2012: 5
- FWCI
- 10.92
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-sa
- Locations
- Biological Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Biodiversity Heritage Library (Smithsonian Institution) (submittedVersion, cc-by-nc-sa)
- Topics
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Biology and Ecology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- settlement cues, metamorphosis, nudibranchs, photobleaching, acridine orange, potassium ions, cesium ions, signal transduction, larval behavior
- MeSH terms
- Animals, Cesium, Fluorescent Dyes, Light, Metamorphosis, Biological, Mollusca, Potassium, Pyridinium Compounds, Sense Organs, Photoreceptor Cells, Invertebrate
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