Conserved MIP receptor–ligand pair regulates Platynereis larval settlement
Open accessAbstract
Life-cycle transitions connecting larval and juvenile stages in metazoans are orchestrated by neuroendocrine signals including neuropeptides and hormones. In marine invertebrate life cycles, which often consist of planktonic larval and benthic adult stages, settlement of the free-swimming larva to the sea floor in response to environmental cues is a key life cycle transition. Settlement is regulated by a specialized sensory–neurosecretory system, the larval apical organ. The neuroendocrine mechanisms through which the apical organ transduces environmental cues into behavioral responses during settlement are not yet understood. Here we show that myoinhibitory peptide (MIP)/allatostatin-B, a pleiotropic neuropeptide widespread among protostomes, regulates larval settlement in the marine annelid Platynereis dumerilii . MIP is expressed in chemosensory–neurosecretory cells in the annelid larval apical organ and signals to its receptor, an orthologue of the Drosophila sex peptide receptor, expressed in neighboring apical organ cells. We demonstrate by morpholino-mediated knockdown that MIP signals via this receptor to trigger settlement. These results reveal a role for a conserved MIP receptor–ligand pair in regulating marine annelid settlement.
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“While useful for gaining spatio-temporal insight into protein activity, the penetration (i.e., effective dosage), affinity, and specificity of smallmolecule inhibitors (often developed for use in other systems) may limit the pathways and targets that can be investigated in vivo in Platynereis.”
BackgroundMethodNeuropeptides control life-phase transitions
Schoofs, L., & Beets, I. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1305724110 · Cited by 27 · see citing works
- Authors
- Conzelmann, M., Williams, E. A., Tunaru, S., Randel, N., Shahidi, R., Asadulina, A., Berger, J., Offermanns, S., & Jékely, G.
- Affiliations
- Max Planck Institute for Developmental Biology (DE)
- Max Planck Institute for Heart and Lung Research (DE)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2013-04-08
- Volume
- 110
- Issue
- 20
- Pages
- 8224-8229
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3657792
- OpenAlex
- W1983885633
- Cited by
- 155
- References
- 65
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 32 · 2023: 15 · 2022: 8 · 2021: 8 · 2020: 12 · 2019: 11 · 2018: 9 · 2017: 8 · 2016: 8 · 2015: 13 · 2014: 12 · 2013: 4
- FWCI
- 6.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Funders
- European Commission (2007–2013, FP7 2007-2013, 260821, FP7/2007)
- Max-Planck-Gesellschaft
- FP7 Ideas: European Research Council (260821)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- myoinhibitory peptides, neuropeptide signaling, larval settlement, Platynereis dumerilii, sex peptide receptor, life cycle transitions, Polychaeta, morpholino knockdown
- MeSH terms
- Animals, Annelida, Dose-Response Relationship, Drug, Image Processing, Computer-Assisted, Larva, Ligands, Microscopy, Electron, Scanning, Molecular Sequence Data, Neurons, Neuropeptides, Signal Transduction, Gene Library, Receptors, Neuropeptide, DNA, Complementary, Expressed Sequence Tags, Receptors, G-Protein-Coupled, Microscopy, Electron, Transmission, Gene Knockdown Techniques
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