Association of Oxidative Stress, Insulin Resistance, and Diabetes Risk Phenotypes
Open accessAbstract
OBJECTIVE—Systemic oxidative stress causes insulin resistance in rodents. We tested the hypothesis that oxidative stress and insulin resistance are associated in humans. RESEARCH DESIGN AND METHODS—We used cross-sectional data from 2,002 nondiabetic subjects of the community-based Framingham Offspring Study. We measured insulin resistance with the homeostasis model and defined categorical insulin resistance as homeostasis model assessment of insulin resistance (HOMA-IR) >75th percentile. We measured oxidative stress using the ratio of urine 8-epi-prostaglandin F2α (8-epi-PGF2α) to creatinine and used age- and sex-adjusted regression models to test the association of oxidative stress with insulin resistance in individuals without diabetes and among subgroups at elevated risk of diabetes. RESULTS—Across 8-epi-PGF2α/creatinine tertiles, the prevalence of insulin resistance increased (18.0, 27.5, and 29.4% for the first, second, and third tertiles, respectively; P < 0.0001), as did mean levels of HOMA-IR (3.28, 3.83, and 4.06 units; P < 0.0001). The insulin resistance–oxidative stress association was attenuated by additional adjustment for BMI (P = 0.06 across tertiles for insulin resistance prevalence; P = 0.004 for mean HOMA-IR). Twenty-six percent of participants were obese (BMI ≥30 kg/m2), 39% had metabolic syndrome (according to the Adult Treatment Panel III definition), and 37% had impaired fasting glucose (IFG) (fasting glucose 5.6–6.9 mmol/l). Among 528 obese participants, respectively, insulin resistance prevalence was 41.3, 60.6, and 54.2% across 8-epi-PGF2α/creatinine tertiles (P = 0.005); among 781 subjects with metabolic syndrome, insulin resistance prevalence was 41.3, 56.7, and 51.7% (P = 0.0025); and among 749 subjects with IFG, insulin resistance prevalence was 39.6, 47.2, and 51.6% (P = 0.04). CONCLUSIONS—Systemic oxidative stress is associated with insulin resistance in individuals at average or elevated risk of diabetes even after accounting for BMI.
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ROSs play a substantive role in insulin resistance and diabetic sequelae, and mitochondrial dysfunction may be an early step.
“Certainly ROSs are known to play a substantive role in both insulin resistance [549-556] and in a variety of diabetic sequelae [95,557-559], and mitochondrial dysfunction may be an early step in this [560].”
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- Authors
- Meigs, J. B., Larson, M. G., Fox, C. S., Keaney, J. F., Vasan, R. S., & Benjamin, E. J.
- Affiliations
- Harvard University (US)
- Boston University (US)
- Brigham and Women's Hospital (US)
- University of Massachusetts Chan Medical School (US)
- ORCID
- Venue
- Diabetes Care
- Publisher
- American Diabetes Association
- Published
- 2007-10-01
- Volume
- 30
- Issue
- 10
- Pages
- 2529-2535
- ISSN
- 0149-5992 (ISSN-L)
- 1935-5548
- OpenAlex
- W2006578694
- Cited by
- 220
- References
- 45
- Citations by year
- 2025: 5 · 2024: 7 · 2023: 14 · 2022: 10 · 2021: 14 · 2020: 13 · 2019: 15 · 2018: 6 · 2017: 15 · 2016: 13 · 2015: 12 · 2014: 13 · 2013: 20 · 2012: 17
- FWCI
- 8.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Diabetes Care (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Diabetes Association
- National Institutes of Health (n01hc25195-41-0-1, n01hv28178-12-0-0)
- National Institute on Aging (R01AG028321, AG-028321)
- National Heart, Lung, and Blood Institute (HL-076784, 2K24 HL 04334, N01 HV28178, N01‐HC25195, K24HL004334, HL71039, R01HL064753, R01HL076784, HL64753, R01HL071039)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- oxidative stress, insulin resistance, diabetes risk, 8-isoprostane, urinary biomarkers, HOMA-IR, metabolic syndrome, impaired fasting glucose, obesity, Framingham Offspring Study, type 2 diabetes prevention, body mass index adjustment, impaired glucose tolerance
- MeSH terms
- Aged, Cardiovascular Diseases, Creatinine, Diabetes Mellitus, Female, Health Surveys, Humans, Insulin Resistance, Male, Massachusetts, Middle Aged, Nuclear Family, Phenotype, Prediabetic State, Dinoprost, Biomarkers, Body Mass Index, Prevalence, Oxidative Stress, Body Size
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