The effect of silver nanoparticles and nystatin on mixed biofilms of Candida glabrata and Candida albicans on acrylic
Abstract
The aim of this study was to compare biofilm formation by Candida glabrata and Candida albicans on acrylic, either individually or when combined (single and dual species) and then examine the antimicrobial effects of silver nanoparticles and nystatin on these biofilms. Candidal adhesion and biofilm assays were performed on acrylic surface in the presence of artificial saliva (AS) for 2 h and 48 h, respectively. Candida glabrata and C. albicans adherence was determined by the number of colony forming units (CFUs) recovered from the biofilms on CHROMagar(®) Candida. In addition, crystal violet (CV) staining was used as an indicator of biofilm biomass and to quantify biofilm formation ability. Pre-formed biofilms were treated either with silver nanoparticles or nystatin and the effect of these agents on the biofilms was evaluated after 24 h. Results showed that both species adhered to and formed biofilms on acrylic surfaces. A significantly (P < 0.05) higher number of CFUs was evident in C. glabrata biofilms compared with those formed by C. albicans. Comparing single and dual species biofilms, equivalent CFU numbers were evident for the individual species. Both silver nanoparticles and nystatin reduced biofilm biomass and the CFUs of single and dual species biofilms (P < 0.05). Silver nanoparticles had a significantly (P < 0.05) greater effect on reducing C. glabrata biofilm biomass compared with C. albicans. Similarly, nystatin was more effective in reducing the number of CFUs of dual species biofilms compared with those of single species (P < 0.05). In summary, C. glabrata and C. albicans can co-exist in biofilms without apparent antagonism, and both silver nanoparticles and nystatin exhibit inhibitory effects on biofilms of these species.
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Silver nanoparticles have a significant effect on reducing C. glabrata biofilm.
“Also, Silva et al. [273] presented results with silver nanoparticles having a significant effect on reducing C. glabrata biofilm.”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [273]
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Candida glabrata Biofilms: How Far Have We Come?
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- Authors
- Silva, S., Pires, P., Monteiro, D. R., Negri, M., Gorup, L. F., Camargo, E. R., Barbosa, D. B., Oliveira, R., Williams, D. W., Henriques, M., & Azeredo, J.
- Affiliations
- Institute for Biotechnology and Bioengineering (PT)
- Universidade Federal de São Carlos (BR)
- Universidade Estadual Paulista (Unesp) (BR)
- Cardiff University (GB)
- ORCID
- Venue
- Medical Mycology
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-02
- Volume
- 51
- Issue
- 2
- Pages
- 178-184
- ISSN
- 0268-1218 (ISSN-L)
- 1369-3786
- 1460-2709
- OpenAlex
- W2159020936
- Cited by
- 101
- References
- 33
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 24 · 2023: 6 · 2022: 6 · 2021: 9 · 2020: 7 · 2019: 6 · 2018: 9 · 2017: 5 · 2016: 11 · 2015: 6 · 2014: 6 · 2013: 3
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medical Mycology (publishedVersion)
- PubMed (publishedVersion)
- Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- UNESP Institutional Repository (São Paulo State University) (submittedVersion)
- Funders
- Fundação para a Ciência e a Tecnologia
- National Institute on Aging
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Dentistry › Orthodontics › Dental materials and restorations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Candida glabrata, Candida albicans, polymicrobial biofilms, silver nanoparticles, nystatin, acrylic resin, dental materials, biofilm inhibition, antifungal agents, fungal adhesion, biofilm biomass
- MeSH terms
- Acrylic Resins, Adhesiveness, Antifungal Agents, Candida albicans, Microscopy, Electron, Scanning, Nystatin, Silver, Colony Count, Microbial, Biofilms, Biomass, Candida glabrata, Nanoparticles
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