Silver colloidal nanoparticles: antifungal effect against adhered cells and biofilms of Candida albicans and Candida glabrata
Abstract
The aim of this study was to evaluate the effect of silver nanoparticles (SN) against Candida albicans and Candida glabrata adhered cells and biofilms. SN (average diameter 5 nm) were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. Minimal inhibitory concentration (MIC) tests were performed for C. albicans (n = 2) and C. glabrata (n = 2) grown in suspension following the Clinical Laboratory Standards Institute microbroth dilution method. SN were applied to adhered cells (2 h) or biofilms (48 h) and after 24 h of contact their effect was assessed by enumeration of colony forming units (CFUs) and quantification of total biomass (by crystal violet staining). The MIC results showed that SN were fungicidal against all strains tested at very low concentrations (0.4-3.3 μg ml(-1)). Furthermore, SN were more effective in reducing biofilm biomass when applied to adhered cells (2 h) than to pre-formed biofilms (48 h), with the exception of C. glabrata ATCC, which in both cases showed a reduction ∼90%. Regarding cell viability, SN were highly effective on adhered C. glabrata and respective biofilms. On C. albicans the effect was not so evident but there was also a reduction in the number of viable biofilm cells. In summary, SN may have the potential to be an effective alternative to conventional antifungal agents for future therapies in Candida-associated denture stomatitis.
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Initial experiments demonstrated increased activity of AgNPs against C. albicans, including preformed biofilms, and most recently this research has expanded to other Candida spp., particularly C. auris.
“While initial experiments demonstrated the increased activity of AgNPs against C. albicans, including preformed biofilms [76,77], most recently this research has also expanded to other Candida spp., and in particular C. auris [78,79].”
From Antifungal therapy of Candida biofilms: Past, present and future (2023) · cites this paper as [77]
Cites
A study of the nucleation and growth processes in the synthesis of colloidal gold
Turkevich, J., Stevenson, P. C., & Hillier, J. (1951) · Discussions of the Faraday Society · doi:10.1039/df9511100055 · Cited by 7,806 · see citing works
Silver nanoparticles as a new generation of antimicrobials
Rai, M., Yadav, A., & Gade, A. (2008) · Biotechnology Advances · doi:10.1016/j.biotechadv.2008.09.002 · Cited by 6,182 · see citing works
Sondi, I., & Salopek‐Sondi, B. (2004) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2004.02.012 · Cited by 5,993 · see citing works
Pal, S., Tak, Y. K., & Song, J. M. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02218-06 · Cited by 4,042 · see citing works
Mechanisms of biofilm resistance to antimicrobial agents
Mah, T.-F. C., & O'Toole, G. A. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(00)01913-2 · Cited by 3,921 · see citing works
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
Asharani, P. V., Low, G. K. M., Hande, M. P., & Valiyaveettil, S. (2008) · ACS Nano · doi:10.1021/nn800596w · Cited by 3,714 · see citing works
Carlson, C., Hussain, S. M., Schrand, A. M., Braydich‐Stolle, L. K., Hess, K. L., Jones, R. L., & Schlager, J. J. (2008) · The Journal of Physical Chemistry B · doi:10.1021/jp712087m · Cited by 1,808 · see citing works
Interaction of silver nanoparticles with HIV-1
Elechiguerra, J. L., Burt, J. L., Morones‐Ramírez, J. R., Camacho-Bragado, A., Gao, X., Lara, H. H., & Yacamán, M. J. (2005) · Journal of Nanobiotechnology · doi:10.1186/1477-3155-3-6 · Cited by 1,713 · see citing works
Chandra, J., Kuhn, D. M., Mukherjee, P. K., Hoyer, L. L., McCormick, T., & Ghannoum, M. A. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.18.5385-5394.2001 · Cited by 1,578 · see citing works
Proteomic Analysis of the Mode of Antibacterial Action of Silver Nanoparticles
Lok, C., Ho, C., Chen, R., He, Q., Yu, W., Sun, H., Tam, P. K. H., Chiu, J., & Che, C. (2006) · Journal of Proteome Research · doi:10.1021/pr0504079 · Cited by 1,577 · see citing works
Antifungal activity of silver nanoparticles against Candida spp.
Panáček, A., Kolář, M., Večeřová, R., Prucek, R., Soukupová, J., Kryštof, V., Hamal, P., Zbořil, R., & Kvı́tek, L. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.07.065 · Cited by 970 · see citing works
Synthesis and Antibacterial Properties of Silver Nanoparticles
Baker, C. C., Pradhan, A. K., Pakstis, L., Pochan, D. J., & Shah, S. İ. (2005) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2005.034 · Cited by 963 · see citing works
Antifungal activity and mode of action of silver nano-particles on Candida albicans
Kim, K., Sung, W. S., Suh, B. K., Moon, S.-K., Choi, J., Kim, J. G., & Lee, D. G. (2008) · BioMetals · doi:10.1007/s10534-008-9159-2 · Cited by 915 · see citing works
Monteiro, D. R., Gorup, L. F., Takamiya, A. S., Filho, A. C. R., Camargo, E. R., & Barbosa, D. B. (2009) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2009.01.017 · Cited by 799 · see citing works
Self-citationKalishwaralal, K., BarathManiKanth, S., Pandian, S. R. K., Deepak, V., & Gurunathan, S. (2010) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2010.04.014 · Cited by 734 · see citing works
<i>Candida</i>Biofilms: an Update
Ramage, G., Saville, S. P., Thomas, D. P., & López-Ribot, J. L. (2005) · Eukaryotic Cell · doi:10.1128/ec.4.4.633-638.2005 · Cited by 678 · see citing works
Denture stomatitis: a role for Candida biofilms
Ramage, G., Tomsett, K., Wickes, B. L., López-Ribot, J. L., & Redding, S. W. (2004) · Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology · doi:10.1016/j.tripleo.2003.04.002 · Cited by 489 · see citing works
Antifungal effect of silver nanoparticles on dermatophytes.
Kim, K.-J., Sung, W. S., Moon, S.-K., Choi, J.-S., Kim, J. G., & Lee, D. G. (2008) · PubMed · Cited by 458 · see citing works
Candida-associated denture stomatitis
Salerno, C., Pascale, M., Contaldo, M., et al. (2011) · Medicina oral, patología oral y cirugía bucal · doi:10.4317/medoral.16.e139 · Cited by 399 · see citing works
The antimicrobial efficacy of polyamide 6/silver-nano- and microcomposites
Damm, C., Münstedt, H., & Rösch, A. (2007) · Materials Chemistry and Physics · doi:10.1016/j.matchemphys.2007.09.002 · Cited by 381 · see citing works
Silver and its application as an antimicrobial agent
Melaiye, A., & Youngs, W. J. (2005) · Expert Opinion on Therapeutic Patents · doi:10.1517/13543776.15.2.125 · Cited by 295 · see citing works
Oral mucosal fungal infections
Samaranayake, L. P., Leung, W. K., & Jin, L. (2008) · Periodontology 2000 · doi:10.1111/j.1600-0757.2008.00291.x · Cited by 279 · see citing works
Coco, B. J., Bagg, J., Cross, L. J., Jose, A., Cross, J., & Ramage, G. (2008) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2008.00439.x · Cited by 276 · see citing works
Balazs, D. J., Triandafillu, K., Wood, P., et al. (2003) · Biomaterials · doi:10.1016/j.biomaterials.2003.08.053 · Cited by 222 · see citing works
Webb, B. C., Thomas, C. J., Willcox, M. D. P., Harty, D. W. S., & Knox, K. W. (1998) · Australian Dental Journal · doi:10.1111/j.1834-7819.1998.tb00157.x · Cited by 217 · see citing works
Biofilm formation in clinical Candida isolates and its association with virulence
Hasan, F., Xess, I., Wang, X., Jain, N., & Fries, B. C. (2009) · Microbes and Infection · doi:10.1016/j.micinf.2009.04.018 · Cited by 206 · see citing works
Silver against Pseudomonas aeruginosa biofilms
Bjarnsholt, T., Kirketerp‐Møller, K., Kristiansen, S., Phipps, R. K., Nielsen, A. K., Jensen, P. Ø., Høiby, N., & Givskov, M. (2007) · Apmis · doi:10.1111/j.1600-0463.2007.apm_646.x · Cited by 198 · see citing works
Stobie, N., Duffy, B., McCormack, D. E., et al. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.10.057 · Cited by 175 · see citing works
Seneviratne, C. J., da Silva, W. J., Jin, L., Samaranayake, Y. H., & Samaranayake, L. P. (2009) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2009.08.002 · Cited by 120 · see citing works
Gorup, L. F., Longo, E., Leite, E. R., et al. (2011) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2011.04.099 · Cited by 117 · see citing works
Self-citationFungicidal activity of miconazole against Candida spp. biofilms
Vandenbosch, D., Braeckmans, K., Nelis, H. J., & Coenye, T. (2010) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkq019 · Cited by 113 · see citing works
Metal resistance in Candida biofilms
Harrison, J. J., Rabiei, M., Turner, R. J., Badry, E. A., Sproule-Willoughby, K. M., & Ceri, H. (2006) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2005.00045.x · Cited by 109 · see citing works
In vitro activity of antifungal combinations against Candida albicans biofilms
Tobudic, S., Kratzer, C., Lassnigg, A. M., Graninger, W. B., & Presterl, E. (2009) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkp429 · Cited by 109 · see citing works
Bagg, J., Sweeney, M. P., Lewis, M. A. O., Jackson, M., Coleman, D. C., Mosaid, A. A., Baxter, W. S., McEndrick, S., & McHugh, S. (2003) · Palliative Medicine · doi:10.1191/0269216303pm793oa · Cited by 107 · see citing works
Denture stomatitis — a review of the aetiology, diagnosis and management
Jeganathan, S., & Lin, C. C. (1992) · Australian Dental Journal · doi:10.1111/j.1834-7819.1992.tb03046.x · Cited by 104 · see citing works
Denture contamination by yeasts in the elderly
Abbeele, A. V., de Meel, H., Ahariz, M., Perraudin, J. P., Beyer, I., & Courtois, P. (2008) · Gerodontology · doi:10.1111/j.1741-2358.2007.00247.x · Cited by 98 · see citing works
In Vitro Biofilm Activity of Non-Candida albicans Candida Species
Silva, S. M., Henriques, M., Oliveira, R., Williams, D. W., & Azeredo, J. (2010) · Current Microbiology · doi:10.1007/s00284-010-9649-7 · Cited by 96 · see citing works
Self-citationFormation of Candida albicans biofilms on non‐shedding oral surfaces
Lamfon, H. A., Porter, S. R., McCullough, M. J., & Pratten, J. R. (2003) · European Journal Of Oral Sciences · doi:10.1111/j.0909-8836.2003.00084.x · Cited by 88 · see citing works
In Vitro Method To Study Antifungal Perfusion in Candida Biofilms
Samaranayake, Y. H., Ye, J., Yau, J. Y. Y., Cheung, B. P. K., & Samaranayake, L. P. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.2.818-825.2005 · Cited by 83 · see citing works
Zheng, J., Wu, X., Wang, M., et al. (2007) · Talanta · doi:10.1016/j.talanta.2007.06.014 · Cited by 79 · see citing works
Proteomics of drug resistance in <i>Candida glabrata</i> biofilms
Seneviratne, C. J., Wang, Y., Jin, L., Abiko, Y., & Samaranayake, L. P. (2010) · PROTEOMICS · doi:10.1002/pmic.200900611 · Cited by 74 · see citing works
Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans
Watamoto, T., Samaranayake, L. P., Jayatilake, J. A. M. S., Egusa, H., Yatani, H., & Seneviratne, C. J. (2009) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2009.03.008 · Cited by 51 · see citing works
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Vazquez-Muñoz, R., Avalos-Borja, M., & Castro-Longoria, E. (2014) · PLoS ONE · doi:10.1371/journal.pone.0108876 · Cited by 168 · see citing works
Lara, H. H., Guisbiers, G., Mendoza, J., Mimun, L. C., Vincent, B., Lopez-Ribot, J. L., & Nash, K. L. (2018) · International Journal of Nanomedicine · doi:10.2147/ijn.s151285 · Cited by 104 · see citing works
Silva, S., Pires, P., Monteiro, D. R., Negri, M., Gorup, L. F., Camargo, E. R., Barbosa, D. B., Oliveira, R., Williams, D. W., Henriques, M., et al. (2013) · Medical Mycology · doi:10.3109/13693786.2012.700492 · Cited by 101 · see citing works
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Vazquez-Munoz, R., Lopez, F. D., & Lopez-Ribot, J. L. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01673 · Cited by 59 · see citing works
Antifungal therapy of Candida biofilms: Past, present and future
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Recent developments on using nanomaterials to combat Candida albicans
Li, B., Pan, L., Zhang, H., Xie, L., Wang, X., Shou, J., Qi, Y., & Yan, X. (2021) · Frontiers in Chemistry · doi:10.3389/fchem.2021.813973 · Cited by 43 · see citing works
- Authors
- Monteiro, D. R., Gorup, L. F., Silva, S., Negri, M., de Camargo, E. R., Oliveira, R., Barbosa, D. B., & Henriques, M.
- Affiliations
- Universidade Estadual Paulista (Unesp) (BR)
- Universidade Federal de São Carlos (BR)
- Institute for Biotechnology and Bioengineering (PT)
- Corresponding author
- Henriques, M.
- Venue
- Biofouling
- Publisher
- Informa UK Limited
- Published
- 2011-07-28
- Volume
- 27
- Issue
- 7
- Pages
- 711-719
- ISSN
- 0892-7014 (ISSN-L)
- 1029-2454
- OpenAlex
- W2097830038
- Cited by
- 205
- References
- 42
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 6 · 2023: 15 · 2022: 20 · 2021: 17 · 2020: 14 · 2019: 22 · 2018: 15 · 2017: 18 · 2016: 15 · 2015: 11 · 2014: 13 · 2013: 15 · 2012: 9
- FWCI
- 8.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biofouling (publishedVersion)
- PubMed (publishedVersion)
- Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
- UNESP Institutional Repository (São Paulo State University) (submittedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Health Sciences › Dentistry › Orthodontics › Dental materials and restorations
- Keywords
- silver nanoparticles, antifungal activity, Candida albicans, Candida glabrata, biofilm, minimum inhibitory concentration, fungicidal activity, denture stomatitis, antifungal agents
- MeSH terms
- Antifungal Agents, Candida albicans, Cell Adhesion, Colloids, Microbial Sensitivity Tests, Silver, Biofilms, Candida glabrata, Metal Nanoparticles, Biofouling
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