l-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes
Open accessAbstract
l-Theanine (γ-glutamylethylamide), a component of green tea, is considered to have regulatory and neuroprotective roles in the brain. The present study was designed to determine the effect of l-theanine on excess dopamine-induced neurotoxicity in both cell culture and animal experiments. The primary cultured mesencephalic neurons or co-cultures of mesencephalic neurons and striatal astrocytes were pretreated with l-theanine for 72 h, and then treated with excess dopamine for further 24 h. The cell viability of dopamine neurons and levels of glutathione were evaluated. Excess dopamine-induced neurotoxicity was significantly attenuated by 72 h preincubation with l-theanine in neuron-astrocyte co-cultures but not in neuron-rich cultures. Exposure to l-theanine increased the levels of glutathione in both astrocytes and glial conditioned medium. The glial conditioned medium from l-theanine-pretreated striatal astrocytes attenuated dopamine-induced neurotoxicity and quinoprotein formation in mesencephalic neurons. In addition, replacement of l-glutamate with l-theanine in an in vitro cell-free glutathione-synthesis system produced glutathione-like thiol compounds. Furthermore, l-theanine administration (4 mg/kg, p.o.) for 14 days significantly increased glutathione levels in the striatum of mice. The results suggest that l-theanine provides neuroprotection against oxidative stress-induced neuronal damage by humoral molecules released from astrocytes, probably including glutathione.
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DNA fragmentation and nerve cell (SH-SY5Y) apoptosis are reduced by L-theanine.
“DNA fragmentation and nerve cell (SH-SY5Y) apoptosis are also reduced by L-the- anine [41][42][43].”
From l -theanine: A potential multifaceted natural bioactive amide as health supplement (2017) · cites this paper as [43]
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- Authors
- Takeshima, M., Miyazaki, I., Murakami, S., Kita, T., & Asanuma, M.
- Affiliations
- Okayama University (JP)
- Kyushu Nutrition Welfare University (JP)
- Corresponding author
- Asanuma, Masato
- Venue
- Journal of Clinical Biochemistry and Nutrition
- Published
- 2016
- Volume
- 59
- Issue
- 2
- Pages
- 93-99
- ISSN
- 0912-0009 (ISSN-L)
- 1880-5086
- PMC
- PMC5018574
- OpenAlex
- W2460913867
- Cited by
- 31
- References
- 40
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 3 · 2018: 2 · 2017: 3
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Journal of Clinical Biochemistry and Nutrition (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (KAKENHI #24111533)
- Japan Society for the Promotion of Science (21591082, 26350170, 16K09673, 22590934, 25461279)
- Topics
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Keywords
- theanine, neuroprotection, astrocytes, astrocyte-neuron co-culture, mesencephalic neurons, glutathione, oxidative stress, glutathione synthesis, striatum
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