Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk
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Lactobacillus plantarum WLPL04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on Caco-2 cells). The results showed that Lb. plantarum WLPL04 had broad-spectrum activity against gram-positive strains (Listeria monocytogenes CMCC54007, Bacillus cereus ATCC14579, and Staphylococcus aureus CMCC26003) and gram-negative strains (Pseudomonas aeruginosa MCC10104, Shigella sonnei ATCC25931, Enterobacter sakazakii ATCC29544, Salmonella typhimurium ATCC13311, and Escherichia coli O157:H7). Antibiotic susceptibility tests showed that Lb. plantarum WLPL04 was susceptible to 8 of 14 antibiotics (e.g., erythromycin and nitrofurantoin) and resistant to 6 of 14 antibiotics (e.g., kanamycin and bacitracin). Lactobacillus plantarum WLPL04 was able to survive at pH 2.5 for 3h and at 0.45% bile salt for 12h, suggesting that it can survive well in the gastrointestinal tract. In addition, the exopolysaccharide yield of Lb. plantarum WLPL04 reached 426.73 ± 65.56 mg/L at 24h. With strategies of competition, inhibition, and displacement, Lb. plantarum WLPL04 reduced the adhesion of E. coli O157:H7 (35.51%), Sal. typhimurium ATCC 13311 (8.10%), and Staph. aureus CMCC 26003 (40.30%) on Caco-2 cells by competition, and subsequently by 59.80, 62.50, and 42.60%, respectively, for the 3 pathogens through inhibition, and by 75.23, 39.97, and 52.88%, respectively, through displacement. Lactobacillus plantarum WLPL04 attenuated the acute stress induced by sodium dodecyl sulfate on Caco-2 cells and significantly inhibited the expression of inflammatory cytokines (IL-6, IL-8 and tumor necrosis factor-α) on Caco-2 cells but increased IL-10 expression in vitro compared with the Salmonella-treated group. In summary, Lb. plantarum WLPL04 from breast milk could be considered as a probiotic candidate for dairy products to promote human health.
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Some lactic acid bacteria isolated from human breast milk have been characterized as potential probiotics, including Lactobacillus casei SR1, L. casei SR2, L. paracasei SR4, and L. plantarum WLPL04.
“In correlation, it has been reported that some LAB isolated from human breast milk were consequently characterized as potential probiotics, such as Lactobacillus casei SR1, L. casei SR2 and Lactobacillus paracasei SR4 [24] and Lactobacillus plantarum WLPL04 [25].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [25]
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Probiotics in the dairy industry—Advances and opportunities
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- Authors
- Jiang, M., Zhang, F., Wan, C., Xiong, Y., Shah, N. P., Wei, H., & Tao, X.
- Affiliations
- Nanchang University (CN)
- University of Hong Kong (HK)
- Corresponding author
- Tao, Xueying
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2016-03
- Volume
- 99
- Issue
- 3
- Pages
- 1736-1746
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2285906411
- Cited by
- 125
- References
- 36
- Citations by year
- 2026: 12 · 2025: 9 · 2024: 14 · 2023: 10 · 2022: 12 · 2021: 21 · 2020: 17 · 2019: 7 · 2018: 13 · 2017: 6 · 2016: 4
- FWCI
- 12.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Funders
- the China Postdoctoral Science Foundation (2015M570567)
- Jiangxi Province Postdoctoral Science Foundation (2014KY07)
- National Natural Science Foundation of China (31170091, 31360377, 31260363)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus plantarum, probiotics, breast milk, pathogen adhesion inhibition, adhesion prevention, antibiotic susceptibility, bile salt tolerance, acid resistance, exopolysaccharides, sodium dodecyl sulfate, anti-inflammatory activity, Caco-2 cells, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7, Salmonella typhimurium, Cronobacter sakazakii
- MeSH terms
- Animals, Female, Gram-Negative Bacteria, Gram-Positive Bacteria, Humans, Milk, Human, Cytokines, Caco-2 Cells, Probiotics, Lactobacillus plantarum, Lactiplantibacillus plantarum
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