Cited by 84Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyFood and Agricultural Sciences

Effect of cellulase and Lactobacillus casei on ensiling characteristics, chemical composition, antioxidant activity, and digestibility of mulberry leaf silage

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He, Liwen, Zhou, Wei, Wang, Cheng, Yang, Fuyu, Chen, Xiaoyang, Zhang, Qing·2019·Journal of Dairy Science

Abstract

With the lack of feed resources in China, mulberry leaves have been developed as a ruminant forage rich in protein and functional components. To make full use of mulberry leaves, including their nutrients and biological activities, we investigated the effects of cellulase and Lactobacillus casei LC on the nutritive value and antioxidant capacity of mulberry leaf silage. Mulberry leaves from 2 locally prevailing high-yield cultivars were separately subjected to ensiling treatments either with or without addition of Lactobacillus culture, in combination with variable levels of cellulase application. After 60-d ensiling, the resulting silage was sampled and analyzed for fermentation parameters, antioxidant activity, carbohydrates, and protein fractions, as well as in vitro rumen gas production. The results showed that nutrients, including functional components of mulberry leaves, were well preserved during the ensiling process. Compared with the control, the application of cellulase and Lactobacillus casei LC resulted in greater dry matter recovery, higher contents of lactic acid, true protein, and total flavonoid, and increased antioxidant activity and microbial protein production, as well as decreased pH value, lower concentrations of ammonium nitrogen, and lower fiber fractions in the mulberry leaf silage, which varied between the 2 selected mulberry cultivars. These results suggest that mulberry leaf silage could be a quality feed with robust antioxidant capacity, which could be improved by the application of cellulase and Lactobacillus casei LC.

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  • This could be due to the CE addition promoting the degradation of fiber in SR and ML silage mixes; on the other hand, it could be due to the degradation of carbohydrates by LAB during silage fermentation to produce CO2, resulting in the reduction of fiber.

    “On the one hand, this could be due to the CE addition promoting the degradation of fiber in SR and ML silage mixes; on the other hand, it could be due to the degradation of carbohydrates by LAB during silage fermentation to produce CO2, resulting in the reduction of fiber.”

    From Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover (2021) · cites this paper as [29]

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Research works printed but not linked: 2

  1. HE ET AL. Journal of Dairy Science Vol. 102 No. 11, 2019
  2. Li, W. 2012. Effects of mulberry leaves on performance, serum bio- chemical parameters, immune function, antioxidation and meat quality in meat sheep. Master's thesis, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

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Authors
He, L., Zhou, W., Wang, C., Yang, F., Chen, X., & Zhang, Q.
Affiliations
  • South China Agricultural University (CN)
  • China Agricultural University (CN)
Corresponding author
  • Chen, Xiaoyang
  • Zhang, Qing
Venue
Journal of Dairy Science
Published
2019-11
Volume
102
Issue
11
Pages
9919-9931
ISSN
0022-0302 (ISSN-L)
OpenAlex
W2969324738
Cited by
84
References
55
Citations by year
2026: 7 · 2025: 12 · 2024: 23 · 2023: 13 · 2022: 14 · 2021: 9 · 2020: 5 · 2019: 1
FWCI
10.18
Type
journal article
Language
en
Open access
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (2017YFD0502102, 31702177)
  • Guangzhou Science, Technology and Innovation Commission (201707010462)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords
cellulase, Lactobacillus casei, silage fermentation, silage additives, antioxidant activity, flavonoids, in vitro rumen fermentation, microbial protein, ruminant forage, dry matter recovery, silage fermentation quality, mulberry cultivars
MeSH terms
Animals, Antioxidants, Carbohydrates, Cellulase, Dietary Fiber, Fermentation, Fruit, Lacticaseibacillus casei, Nitrogen, Nutritive Value, Rumen, Silage, Plant Leaves, Morus

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