Cited by 456Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsBiopolymer Synthesis and Applications

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Invited Review: Methods for the Screening, Isolation, and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria

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Ruas-Madiedo, P., de los Reyes-Gavilán, C.G.·2005·Journal of Dairy Science

DOI 10.3168/jds.s0022-0302(05)72750-8

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Abstract

The ability to produce exopolysaccharides (EPS) is widespread among lactic acid bacteria (LAB), although the physiological role of these molecules has not been clearly established yet. Some EPS confer on LAB a "ropy" character that can be detected in cultures that form long strands when extended with an inoculation loop. When EPS are produced in situ during milk fermentation they can act as natural biothickeners, giving the product a suitable consistency, improving viscosity, and reducing syneresis. In addition, some of these EPS may have beneficial effects on human health. The increasing demand by consumers of novel dairy products requires a better understanding of the effect of EPS on existing products and, at the same time, the search for new EPS-producing strains with desirable properties. The use of genetically modified organisms capable of producing high levels of EPS or newly designed biopolymers is still very limited. Therefore, exploration of the biodiversity of wild LAB strains from natural ecological environments is currently the most suitable approach to search for the desired EPS-phenotype. The screening of ropy strains and the isolation and characterization of EPS responsible for this characteristic have led to the application over the past years of a wide variety of techniques. This review summarizes the available information on methods and procedures used for research on this topic. The information provided deals with methods for screening of EPS-producing LAB, detection of the ropy phenotype, and the physicochemical and structural characterization of these molecules, including parameters related to their viscosifying properties. To our knowledge, this is the first compilation of methods available for the study of EPS produced by LAB.

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See all 456

Authors: Ruas-Madiedo, P., & de los Reyes-Gavilán, C. G.
Venue: Journal of Dairy Science
Publisher: American Dairy Science Association
Published: 2005-03
Volume: 88
Issue: 3
Pages: 843-856
ISSN: 0022-0302 (ISSN-L)
DOI: 10.3168/jds.s0022-0302(05)72750-8
OpenAlex: W2004400025
Cited by: 456
References: 135
Citations by year: 2026: 19 · 2025: 28 · 2024: 23 · 2023: 30 · 2022: 35 · 2021: 37 · 2020: 31 · 2019: 20 · 2018: 31 · 2017: 19 · 2016: 21 · 2015: 26 · 2014: 25 · 2013: 15 · 2012: 17
FWCI: 1.75
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 5069 d)
Locations: Journal of Dairy Science (publishedVersion)
PubMed (publishedVersion)
Funders: FEDER funds
Spanish “Plan Nacional de I+D+I”
“Fundación Príncipe de Asturias”
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
Keywords: exopolysaccharides, lactic acid bacteria, dairy fermentation, syneresis, dairy products
MeSH terms: Dairy Products, Fermentation, Food Microbiology, Lactobacillus, Polysaccharides, Bacterial, Viscosity, Probiotics, Organisms, Genetically Modified

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Invited Review: Methods for the Screening, Isolation, and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria

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