Cited by 217Microbial Metabolites in Food BiotechnologyProbiotics and Fermented FoodsInfant Nutrition and Health

In Vitro Study of Prebiotic Properties of Levan-Type Exopolysaccharides from Lactobacilli and Non-Digestible Carbohydrates Using Denaturing Gradient Gel Electrophoresis

Bello, Fabio Dal, Walter, Jens, Hertel, Christian, Hammes, Walter P.·2001·Systematic and Applied Microbiology

Abstract

Batch cultures inoculated with human faeces were used to study the prebiotic properties of levan-type exopolysaccharides (EPS) from Lactobacillus sanfranciscensis as well as levan, inulin, and fructooligosaccharide (FOS). Denaturing gradient gel electrophoresis of 16S rDNA fragments generated by PCR with universal primers was used to analyse the cultures. Characteristic changes were revealed in the composition of the gut bacteria during fermentation of the carbohydrates. An enrichment of Bifidobacterium spp. was found for the EPS and inulin but not for levan and FOS. The bifidogenic effect of the EPS was confirmed by culturing on selective medium. In addition, the use of EPS and FOS resulted in enhanced growth of Eubacterium biforme and Clostridium perfringens, respectively.

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Crossref · 10.1078/0723-2020-00033

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Authors
Bello, F. D., Walter, J., Hertel, C., & Hammes, W. P.
Affiliations
University of Hohenheim (DE)
Venue
Systematic and Applied Microbiology
Publisher
Elsevier BV
Published
2001
Volume
24
Issue
2
Pages
232-237
ISSN
0723-2020 (ISSN-L)
OpenAlex
W2081966773
Cited by
214
References
30
Citations by year
2026: 1 · 2025: 8 · 2024: 4 · 2023: 12 · 2022: 10 · 2021: 14 · 2020: 14 · 2019: 13 · 2018: 14 · 2017: 8 · 2016: 4 · 2015: 16 · 2014: 12 · 2013: 9 · 2012: 12
FWCI
3.65
Type
journal article
Language
en
Open access
closed
Locations
  • Systematic and Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
prebiotics, levan, exopolysaccharides, Lactobacillus sanfranciscensis, non-digestible carbohydrates, inulin, fructooligosaccharides, gut microbiota, Bifidobacterium, bifidogenic effect, fecal fermentation, 16S rRNA gene, Clostridium perfringens
MeSH terms
Culture Media, Electrophoresis, Agar Gel, Fermentation, Fructans, Humans, Intestines, Inulin, Lactobacillus, Oligosaccharides, Polysaccharides, RNA, Ribosomal, 16S, Colony Count, Microbial, Polymerase Chain Reaction, Probiotics

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