Cited by 456Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsBiopolymer Synthesis and Applications

The DOI matches its Crossref record; 129 of the 135 references it cites were found in a registry or an index.

Invited Review: Methods for the Screening, Isolation, and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria

Open access

Ruas-Madiedo, P., de los Reyes-Gavilán, C.G.·2005·Journal of Dairy Science

DOI 10.3168/jds.s0022-0302(05)72750-8

Open this paper in CoChat — free account Open PDF

Abstract

The ability to produce exopolysaccharides (EPS) is widespread among lactic acid bacteria (LAB), although the physiological role of these molecules has not been clearly established yet. Some EPS confer on LAB a "ropy" character that can be detected in cultures that form long strands when extended with an inoculation loop. When EPS are produced in situ during milk fermentation they can act as natural biothickeners, giving the product a suitable consistency, improving viscosity, and reducing syneresis. In addition, some of these EPS may have beneficial effects on human health. The increasing demand by consumers of novel dairy products requires a better understanding of the effect of EPS on existing products and, at the same time, the search for new EPS-producing strains with desirable properties. The use of genetically modified organisms capable of producing high levels of EPS or newly designed biopolymers is still very limited. Therefore, exploration of the biodiversity of wild LAB strains from natural ecological environments is currently the most suitable approach to search for the desired EPS-phenotype. The screening of ropy strains and the isolation and characterization of EPS responsible for this characteristic have led to the application over the past years of a wide variety of techniques. This review summarizes the available information on methods and procedures used for research on this topic. The information provided deals with methods for screening of EPS-producing LAB, detection of the ropy phenotype, and the physicochemical and structural characterization of these molecules, including parameters related to their viscosifying properties. To our knowledge, this is the first compilation of methods available for the study of EPS produced by LAB.

Integrity signals

Registry

Crossref · 10.3168/jds.s0022-0302(05)72750-8

Cites

129 of 129 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Miller, G. L. (1959) · Analytical Chemistry · doi:10.1021/ac60147a030 · Cited by 28,963
Brock Biology of Microorganisms
Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A. (1996) · Cited by 5,759
A simple and rapid preparation of alditol acetates for monosaccharide analysis
Blakeney, A. B., Harris, P. J., Henry, R. J., & Stone, B. (1983) · Carbohydrate Research · doi:10.1016/0008-6215(83)88244-5 · Cited by 1,971
Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent
Morris, D. L. (1948) · Science · doi:10.1126/science.107.2775.254 · Cited by 1,945
Determination of the d and l configuration of neutral monosaccharides by high-resolution capillary g.l.c.
Gerwig, G. J., Kamerling, J. P., & Vliegenthart, J. F. G. (1978) · Carbohydrate Research · doi:10.1016/s0008-6215(00)80881-2 · Cited by 859
Heteropolysaccharides from lactic acid bacteria
Vuyst, L. D., & Degeest, B. (1999) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1999.tb00395.x · Cited by 840
Novel and established applications of microbial polysaccharides
Sutherland, I. W. (1998) · Trends in biotechnology · doi:10.1016/s0167-7799(97)01139-6 · Cited by 678
An overview of the functionality of exopolysaccharides produced by lactic acid bacteria
Ruas‐Madiedo, P., Hugenholtz, J., & Zoon, P. (2002) · International Dairy Journal · doi:10.1016/s0958-6946(01)00160-1 · Cited by 640
Self-citation
Determination of the absolute configuration of monosaccharides in complex carbohydrates by capillary g.l.c.
Gerwig, G. J., Kamerling, J. P., & Vliegenthart, J. F. G. (1979) · Carbohydrate Research · doi:10.1016/s0008-6215(00)83788-x · Cited by 614

Show all 135 references

Research works printed but not linked: 3

Mozzi, 1995, Influence of temperature on the production of exopolysaccharides by thermophilic lactic acid bacteria, Milchwissenschaft, 50, 80
Stingele, 1995, Homologous integration and transposition of identify genes involved in exopolysaccharide production in Streptococcus thermophilus, Dev. Biol. Standards, 85, 487
Urashima, 1999, Exocellular polysaccharide produced by Streptococcus thermophilus, Milchwissenschaft, 54, 190

Sorted by citations

Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry
Wang, Y., Wu, J., Lv, M., Shao, Z., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y., & Geng, W. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.612285 · Cited by 1,001
Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly
Pereira, S. B., Zille, A., Micheletti, E., Moradas‐Ferreira, P., Philippis, R. D., & Tamagnini, P. (2009) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2009.00183.x · Cited by 660
Exopolysaccharides from Probiotic Bacteria and Their Health Potential
Angelin, J., & Kavitha, M. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.06.190 · Cited by 617
New Perspectives for Lactobacilli Exopolysaccharides
Badel, S., Bernardi, T., & Michaud, P. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.011 · Cited by 497
Exopolysaccharides Produced by Lactic Acid Bacteria: From Health-Promoting Benefits to Stress Tolerance Mechanisms
Caggianiello, G., Kleerebezem, M., & Spano, G. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7471-2 · Cited by 396
Postbiotics: Current Trends in Food and Pharmaceutical Industry
Thorakkattu, P., Khanashyam, A. C., Shah, K., Babu, K. S., Mundanat, A. S., Deliephan, A., Deokar, G. S., Santivarangkna, C., & Nirmal, N. P. (2022) · Foods · doi:10.3390/foods11193094 · Cited by 438
Production, Properties, and Industrial Food Application of Lactic Acid Bacteria-Derived Exopolysaccharides
Zannini, E., Waters, D. M., Coffey, A., & Arendt, E. K. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-7172-2 · Cited by 371
Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties
Jurášková, D., Ribeiro, S. C., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11020156 · Cited by 342
Silage processing and strategies to prevent persistence of undesirable microorganisms.
Dunière, L., Sindou, J., Chaucheyras-Durand, F., Chevallier, I., & Thévenot-Sergentet, D. (2013) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2013.04.006 · Cited by 335
Polysaccharide Production by Lactic Acid Bacteria: From Genes to Industrial Applications
Zeidan, A. A., Poulsen, V. K., Janzen, T., Buldo, P., Derkx, P. M. F., Øregaard, G., & Neves, A. R. (2017) · FEMS Microbiology Reviews · doi:10.1093/femsre/fux017 · Cited by 326
Characterization of a Novel Exopolysaccharide with Antitumor Activity from Lactobacillus plantarum 70810
Wang, K., Li, W., Rui, X., Chen, X., Jiang, M., & Dong, M. (2014) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2013.10.036 · Cited by 325
Synthesis, Production, and Biotechnological Applications of Exopolysaccharides and Polyhydroxyalkanoates by Archaea
Poli, A., Donato, P. D., Abbamondi, G. R., & Nicolaus, B. (2011) · Archaea · doi:10.1155/2011/693253 · Cited by 316
Glucansucrases: Three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications
Leemhuis, H., Pijning, T., Dobruchowska, J. M., van Leeuwen, S. S., Kralj, S., Dijkstra, B. W., & Dijkhuizen, L. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.06.037 · Cited by 320
Lactic Acid Bacteria Exopolysaccharides in Foods and Beverages: Isolation, Properties, Characterization, and Health Benefits
Lynch, K. M., Zannini, E., Coffey, A., & Arendt, E. K. (2018) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-030117-012537 · Cited by 289
Exopolysaccharides from extremophiles: from fundamentals to biotechnology
Nicolaus, B., Kambourova, M. S., & Öner, E. T. (2010) · Environmental Technology · doi:10.1080/09593330903552094 · Cited by 283
Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions
Coelho, M. C., Malcata, F. X., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11152276 · Cited by 276
“Some strains of the genus Bifidobacterium are also capable of producing these biopolymers [298,300].”
Influential
Potentials of Exopolysaccharides from Lactic Acid Bacteria
Patel, S. V., Majumder, A., & Goyal, A. (2011) · Indian Journal of Microbiology · doi:10.1007/s12088-011-0148-8 · Cited by 274
Environmental applications of microbial extracellular polymeric substance (EPS): A review
Siddharth, T., Sridhar, P., Vinila, V., & Tyagi, R. D. (2021) · Journal of Environmental Management · doi:10.1016/j.jenvman.2021.112307 · Cited by 272
Bacterial exopolysaccharides as emerging bioactive macromolecules: from fundamentals to applications
Kaur, N., & Dey, P. (2022) · Research in Microbiology · doi:10.1016/j.resmic.2022.104024 · Cited by 285
Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications
Nguyen, P.-T., Nguyen, T.-T., Bui, D., Hong, P.-T., Hoang, Q.-K., & Nguyen, H. (2020) · AIMS Microbiology · doi:10.3934/microbiol.2020027 · Cited by 279
Exopolysaccharides Produced by Probiotic Strains Modify the Adhesion of Probiotics and Enteropathogens to Human Intestinal Mucus
Ruas‐Madiedo, P., Gueimonde, M., Margollés, A., Reyes‐Gavilán, C. G. D. L., & Salminen, S. (2006) · Journal of Food Protection · doi:10.4315/0362-028x-69.8.2011 · Cited by 244
Self-citation
Influence of exopolysaccharides on the structure, texture, stability and sensory properties of yoghurt and related products
Mende, S., Rohm, H., & Jaros, D. (2015) · International Dairy Journal · doi:10.1016/j.idairyj.2015.08.002 · Cited by 238
Contributions of exopolysaccharides from lactic acid bacteria as biotechnological tools in food, pharmaceutical, and medical applications
Daba, G. M., Elnahas, M. O., & Elkhateeb, W. A. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.01.110 · Cited by 241
Current status of biotechnological production and applications of microbial exopolysaccharides
Barcelos, M. C. S., Vespermann, K. A. C., Pelissari, F. M., & Molina, G. (2019) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2019.1575791 · Cited by 226
Polysaccharides: bowel health and gut microbiota
Do, M. H., Seo, Y.-S., & Park, H. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1755949 · Cited by 231
Food and health applications of exopolysaccharides produced by lactic acid bacteria
JB Prajapat, A. P. (2013) · Advances in Dairy Research · doi:10.4172/2329-888x.1000107 · Cited by 219
Adherence factors ofLactobacillusin the human gastrointestinal tract
Vélez, M. P., Keersmaecker, S. C. J. D., & Vanderleyden, J. (2007) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2007.00908.x · Cited by 207
A review on preparation and chemical analysis of postbiotics from lactic acid bacteria
Moradi, M., Molaei, R., & Guimarães, J. T. (2020) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2020.109722 · Cited by 225
Biopolymers from Lactic Acid Bacteria. Novel Applications in Foods and Beverages
Torino, M. I., Font de Valdez, G., & Mozzi, F. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00834 · Cited by 201
NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa
Maina, N. H., Tenkanen, M., Maaheimo, H., Juvonen, R., & Virkki, L. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.04.012 · Cited by 200
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194
“In certain cases, heat treatment is used to improve the EPS recovery from the culture medium as the first step in EPS isolation [27,32,35,58,97,98].”
BackgroundMethodInfluential
Natural flocculants for the treatment of wastewaters containing dyes or heavy metals: A state-of-the-art review
Gaayda, J. E., Titchou, F. E., Oukhrib, R., Yap, P., Liu, T., Hamdani, M., & Akbour, R. A. (2021) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2021.106060 · Cited by 198
Exopolysaccharides of Lactic Acid Bacteria: Production, Purification and Health Benefits towards Functional Food
Sørensen, H. M., Rochfort, K. D., Maye, S., Macleod, G. H. B., Brabazon, D., Loscher, C. E., & Freeland, B. H. (2022) · Nutrients · doi:10.3390/nu14142938 · Cited by 202
Exopolysaccharides Produced by Lactic Acid Bacteria and Bifidobacteria: Structures, Physiochemical Functions and Applications in the Food Industry
Xu, Y., Cui, Y., Yue, F., Liu, L., Shan, Y., Liu, B., Zhou, Y., & Lü, X. (2019) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2019.03.032 · Cited by 189
Higher order structure of (1,3)‐β‐ D ‐glucans and its influence on their biological activities and complexation abilities
Sletmoen, M., & Stokke, B. T. (2008) · Biopolymers · doi:10.1002/bip.20920 · Cited by 198
CRISPR-Cas9 D10A Nickase-Assisted Genome Editing in Lactobacillus casei
Song, X., Huang, H., Xiong, Z., Ai, L., & Yang, S. (2017) · Applied and Environmental Microbiology · doi:10.1128/aem.01259-17 · Cited by 186
The anti-cancer effects and mechanisms of lactic acid bacteria exopolysaccharides in vitro: A review
Wu, J., Zhang, Y., Ye, L., & Wang, C. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.117308 · Cited by 178
Glucan and Fructan Production by Sourdough Weissella cibaria and Lactobacillus plantarum
Cagno, R. D., Angelis, M. D., Limitone, A., Minervini, F., Carnevali, P., Corsetti, A., Gänzle, M. G., Ciati, R., & Gobbetti, M. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf061393+ · Cited by 164
Films based on kefiran, an exopolysaccharide obtained from kefir grain: Development and characterization
Piermaría, J., Pinotti, A. N., García, M. A., & Abraham, A. G. (2008) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2008.05.003 · Cited by 163
Biomedical Applications of Bacterial Exopolysaccharides: A Review
Nadzir, M. M., Nurhayati, R. W., Idris, F. N., & Nguyen, M. H. (2021) · Polymers · doi:10.3390/polym13040530 · Cited by 170
ADSA Foundation Scholar Award: Possibilities and Challenges of Exopolysaccharide-Producing Lactic Cultures in Dairy Foods
Hassan, A. N. (2008) · Journal of Dairy Science · doi:10.3168/jds.2007-0558 · Cited by 151
The effect of cell surface components on adhesion ability of Lactobacillus rhamnosus
Polak‐Berecka, M., Waśko, A. D., Paduch, R., Skrzypek, T., & Sroka‐Bartnicka, A. (2014) · Antonie van Leeuwenhoek · doi:10.1007/s10482-014-0245-x · Cited by 150
Association of Beta-Glucan Endogenous Production with Increased Stress Tolerance of Intestinal Lactobacilli
Stack, H. M., Kearney, N., Stanton, C., Fitzgerald, G. F., & Ross, R. P. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01524-09 · Cited by 145
Diversity of Heteropolysaccharide-Producing Lactic Acid Bacterium Strains and Their Biopolymers
Mozzi, F., Vaningelgem, F., Hebert, E. M., der Meulen, R. V., Foulquié-Moreno, M. R., de Valdez, G. F., & Vuyst, L. D. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.02780-05 · Cited by 145
A review of bread qualities and current strategies for bread bioprotection: Flavor, sensory, rheological, and textural attributes
Dong, Y., & Karboune, S. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12717 · Cited by 150
Postbiotics: Functional Food Materials and Therapeutic Agents for Cancer, Diabetes, and Inflammatory Diseases
Gurunathan, S., Pratheep, T., & Kim, J. (2023) · Foods · doi:10.3390/foods13010089 · Cited by 159
Characterization of lactic acid bacteria isolated from wheat bran sourdough
Manini, F., Casiraghi, M. C., Poutanen, K. S., Brasca, M., Erba, D., & Plumed‐Ferrer, C. (2015) · LWT · doi:10.1016/j.lwt.2015.10.045 · Cited by 137
Sea Ice Microorganisms: Environmental Constraints and Extracellular Responses
Ewert, M., & Deming, J. W. (2013) · Biology · doi:10.3390/biology2020603 · Cited by 130
Effect of Lactic Acid Bacteria Fermentation on Plant-Based Products
Yang, X., Hong, J., Wang, L., Cai, C., Mo, H., Wang, J., Fang, X., & Liao, Z. (2024) · Fermentation · doi:10.3390/fermentation10010048 · Cited by 148
Influence of lupin-based milk alternative heat treatment and exopolysaccharide-producing lactic acid bacteria on the physical characteristics of lupin-based yogurt alternatives
Hickisch, A., Beer, R., Vogel, R. F., & Toelstede, S. (2016) · Food Research International · doi:10.1016/j.foodres.2016.03.037 · Cited by 119
New Insights into Various Production Characteristics of Streptococcus thermophilus Strains
Cui, Y., Xu, T., Qu, X., Hu, T., Jiang, X., & Zhao, C. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17101701 · Cited by 120
Novel isolates of lactobacilli from fermented Portuguese olive as potential probiotics
Peres, C. M., Alves, M., Hernández‐Mendoza, A., Moreira, L., da Silva, S. R. A., Bronze, M. R., Vilas-Boas, L., Peres, C., & Malcata, F. X. (2014) · LWT · doi:10.1016/j.lwt.2014.03.003 · Cited by 119
Lactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis
Espeche, M. C., Pellegrino, M., Frola, I. D., Larriestra, A. J., Bogni, C. I., & Nader-Macı́as, M. E. (2012) · Anaerobe · doi:10.1016/j.anaerobe.2012.01.002 · Cited by 119
Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110
Mota, R., Guimarães, R., Büttel, Z., Rossi, F., Colica, G., Silva, C., Santos, C., Gales, L., Zille, A., Philippis, R. D., et al. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.10.070 · Cited by 112
Purification, characterization and antioxidant activity of the exopolysaccharide from Weissella cibaria SJ14 isolated from Sichuan paocai
Zhu, Y., Wang, C., Jia, S., Wang, B., Zhou, K., Chen, S., Yang, Y., & Liu, S. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.04.067 · Cited by 108
Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
Notararigo, S., Nachér-Vázquez, M., Ibarburu, I., Werning, M. L., de Palencia, P. F., Dueñas, M. T., Aznar, R., López, P., & Prieto, A. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.05.016 · Cited by 107
Overview of exopolysaccharides produced by Weissella genus – A review
Kavitake, D., Devi, P. B., & Shetty, P. H. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.08.185 · Cited by 108
THE INTERNATIONAL JOURNAL OF HUMANITIES & SOCIAL STUDIES
Astuti, K. S., & Prabawa, S. P. A. K. U. K. U. H. (2021) · Journal of Humanities and Social Studies · Cited by 104
Recent Advances in the Production of Exopolysaccharide (EPS) from Lactobacillus spp. and Its Application in the Food Industry: A Review
Bibi, A., Xiong, Y., Rajoka, M. S. R., Mehwish, H. M., Radicetti, E., Umair, M., Shoukat, M., Khan, M. K. I., & Aadil, R. M. (2021) · Sustainability · doi:10.3390/su132212429 · Cited by 108
Dextrans produced by lactic acid bacteria exhibit antiviral and immunomodulatory activity against salmonid viruses
Nachér-Vázquez, M., Ballesteros, N. A., Canales, Á., Saint-Jean, S. S. R., Pérez-Prieto, S. I., Prieto, A., Aznar, R., & López, P. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.02.020 · Cited by 102
An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
Paulo, E. M., Vasconcelos, M. P., Oliveira, I. S., Affe, H. M. D. J., Nascimento, R., de Melo, I. S., Roque, M. R. D. A., & de Assis, S. A. (2012) · Food Science and Technology · doi:10.1590/s0101-20612012005000094 · Cited by 100
Probiotic Lactobacillus casei Zhang improved the properties of stirred yogurt
Bai, M., Huang, T., Guo, S., Wang, Y., Wang, J., Kwok, L., Tong, D., Zhang, H., & Bilige, M. (2020) · Food Bioscience · doi:10.1016/j.fbio.2020.100718 · Cited by 100
Functional and health-promoting properties of probiotics’ exopolysaccharides; isolation, characterization, and applications in the food industry
Pourjafar, H., Ansari, F., Sadeghi, A., Samakkhah, S. A., & Jafari, S. M. (2022) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2022.2047883 · Cited by 103
Determining probiotic potential of exopolysaccharide producing lactic acid bacteria isolated from vegetables and traditional Indian fermented food products
Patel, A. R., Prajapati, J. B., Holst, O., & Ljungh, Å. (2013) · Food Bioscience · doi:10.1016/j.fbio.2013.10.002 · Cited by 98
Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
der Meulen, R. V., Grosu‐Tudor, S., Mozzi, F., Vaningelgem, F., Zamfir, M., de Valdez, G. F., & Vuyst, L. D. (2007) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2007.07.014 · Cited by 98
Diversity and Antimicrobial Properties of Lactic Acid Bacteria Isolated from Rhizosphere of Olive Trees and Desert Truffles of Tunisia
Fhoula, I., Najjari, A., Turki, Y., Jaballah, S., Boudabous, A., & Ouzari, H. (2013) · BioMed Research International · doi:10.1155/2013/405708 · Cited by 98
Advances in Production and Simplified Methods for Recovery and Quantification of Exopolysaccharides for Applications in Food and Health
Leroy, F., & De Vuyst, L. (2016) · Journal of Dairy Science · doi:10.3168/jds.2015-9936 · Cited by 96
Probiotic and technological properties of exopolysaccharide producing lactic acid bacteria isolated from cereal-based nigerian fermented food products
Adesulu-Dahunsi, A. T., Jeyaram, K., & Sanni, A. I. (2018) · Food Control · doi:10.1016/j.foodcont.2018.04.062 · Cited by 94
Gut health benefit and application of postbiotics in animal production
Zhong, Y., Wang, S., Di, H., Deng, Z., Liu, J., & Wang, H. (2022) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-022-00688-1 · Cited by 102
Bifidobacteria cell wall-derived exo-polysaccharides, lipoteichoic acids, peptidoglycans, polar lipids and proteins – their chemical structure and biological attributes
Pyclik, M. J., Šrůtková, D., Schwarzer, M., & Górska, S. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.12.227 · Cited by 94
Immunoregulatory potential of exopolysaccharide from Lactobacillus rhamnosus KL37. Effects on the production of inflammatory mediators by mouse macrophages
Ciszek‐Lenda, M., Nowak, B., Śróttek, M., Gamian, A., & Marcinkiewicz, J. (2011) · International Journal of Experimental Pathology · doi:10.1111/j.1365-2613.2011.00788.x · Cited by 92
Addition of purified exopolysaccharide isolates from S. thermophilus to milk and their impact on the rheology of acid gels
Mende, S., Peter, M., Bartels, K., Rohm, H., & Jaros, D. (2013) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2012.12.011 · Cited by 91
Methods to identify the unexplored diversity of microbial exopolysaccharides
Rühmann, B., Schmid, J., & Sieber, V. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00565 · Cited by 89
Exopolysaccharides produced byLactobacillussp.: Biosynthesis and applications
Oleksy, M., & Klewicka, E. (2016) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2016.1187112 · Cited by 93
Newly isolated microorganisms with potential application in biotechnology
Pessôa, M. G., Vespermann, K. A. C., Paulino, B. N., Barcelos, M. C. S., Pastore, G. M., & Molina, G. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.01.007 · Cited by 86
Culture Conditions Determine the Balance between Two Different Exopolysaccharides Produced by Lactobacillus pentosus LPS26
Sánchez, J.-I., Martı́nez, B., Guillén, R., Jiménez‐Díaz, R., & Rodrı́guez, A. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.01078-06 · Cited by 84
Physicochemical and antioxidant properties of a gastroprotective exopolysaccharide produced by Streptococcus thermophilus CRL1190
Lobo, R. E., Gómez, M. I., de Valdez, G. F., & Torino, M. I. (2019) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2019.05.036 · Cited by 83
Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria
Ziadi, M., Bouzaiene, T., M’Hir, S., Zaafouri, K., Mokhtar, F., Hamdi, M., & Boisset-Helbert, C. (2018) · BioMed Research International · doi:10.1155/2018/1896240 · Cited by 82
Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk
Nambiar, R. B., Sellamuthu, P. S., Perumal, A. B., Sadiku, E. R., Phiri, G., & Jayaramudu, J. (2018) · Process Biochemistry · doi:10.1016/j.procbio.2018.07.018 · Cited by 81
Bioactive compounds in fermented foods: Health benefits, safety, and future perspectives
Fitsum, S., Gebreyohannes, G., & Sbhatu, D. B. (2025) · Applied Food Research · doi:10.1016/j.afres.2025.101097 · Cited by 91
Potential functions and applications of diverse microbial exopolysaccharides in marine environments
Ibrahim, H. A. H., El-Hassayeb, H. E. A., & El-Sayed, W. M. M. (2022) · Journal of Genetic Engineering and Biotechnology · doi:10.1186/s43141-022-00432-2 · Cited by 84
Prevention of chronic gastritis by fermented milks made with exopolysaccharide-producing Streptococcus thermophilus strains
Rodríguez, M. C., Medici, M., Rodriguez, A. V., Mozzi, F., & de Valdez, G. F. (2009) · Journal of Dairy Science · doi:10.3168/jds.2008-1724 · Cited by 78
Genomic Comparison of Lactobacillus helveticus Strains Highlights Probiotic Potential
Fontana, A., Falasconi, I., Molinari, P., Treu, L., Basile, A., Vezzi, A., Campanaro, S., & Morelli, L. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01380 · Cited by 81
New advances in exopolysaccharides production of Streptococcus thermophilus
Cui, Y., Jiang, X., Hao, M., Qu, X., & Hu, T. (2017) · Archives of Microbiology · doi:10.1007/s00203-017-1366-1 · Cited by 75
Lactic acid bacteria-derived α-glucans: From enzymatic synthesis to miscellaneous applications
Chen, Z., Ni, D., Zhang, W., Stressler, T., & Mu, W. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107708 · Cited by 74
Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains
Pachekrepapol, U., Lucey, J. A., Gong, Y., Naran, R., & Azadi, P. (2017) · Journal of Dairy Science · doi:10.3168/jds.2016-12125 · Cited by 73
Biopolymers Produced by Lactic Acid Bacteria: Characterization and Food Application
Nicolescu, C. M., Bumbac, M., Buruleanu, C. L., Popescu, E. C., Stănescu, S. G., Georgescu, A. A., & Toma, S. M. (2023) · Polymers · doi:10.3390/polym15061539 · Cited by 77
Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC ‐23 isolated from Italian ryegrass
Soundharrajan, I., Vijayakumar, M., Kim, D., Arasu, M. V., Park, H. S., Sivanesan, R., & Choi, K. C. (2015) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.7128 · Cited by 70
Genomic Evolution of Domesticated Microorganisms
Douglas, G. L., & Klaenhammer, T. R. (2010) · Annual Review of Food Science and Technology · doi:10.1146/annurev.food.102308.124134 · Cited by 74
Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
Moghannem, S. A. M., Farag, M. M. S., Shehab, A., & Azab, M. S. (2018) · Brazilian Journal of Microbiology · doi:10.1016/j.bjm.2017.05.012 · Cited by 74
Lactococcus lactis in Dairy Fermentation—Health-Promoting and Probiotic Properties
Kondrotienė, K., Zavistanavičiūtė, P., Aksomaitienė, J., Novoslavskij, A., & Malakauskas, M. (2023) · Fermentation · doi:10.3390/fermentation10010016 · Cited by 83
Technological, rheological and sensory characterizations of a yogurt containing an exopolysaccharide extract from Lactobacillus fermentum Lf2, a new food additive
Ale, E. C., Perezlindo, M. J., Pavón, Y. L., Peralta, G. H., Costa, S. C., Sabbag, N. G., Bergamini, C. V., Reinheimer, J. A., & Binetti, A. G. (2016) · Food Research International · doi:10.1016/j.foodres.2016.10.045 · Cited by 68
Yogurt: The Product and its Manufacture
Corrieu, G., & Béal, C. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-384947-2.00766-2 · Cited by 68
Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces
Jin, H. Z., Jeong, Y., Yoo, S., Johnston, T. V., Ku, S., & Ji, G. E. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1158-1 · Cited by 65
Production, Characterization, and Antioxidant Activities of an Exopolysaccharide Extracted from Spent Media Wastewater after Leuconostoc mesenteroides WiKim32 Fermentation
Choi, I. S., Ko, S. H., Lee, M. E., Kim, H. M., Yang, J. E., Jeong, S.-G., Lee, K. H., Chang, J. Y., Kim, J., & Park, H. W. (2021) · ACS Omega · doi:10.1021/acsomega.0c06095 · Cited by 68
Sugar source modulates exopolysaccharide biosynthesis in Bifidobacterium longum subsp. longum CRC 002
Audy, J., Labrie, S., Roy, D., & LaPointe, G. (2009) · Microbiology · doi:10.1099/mic.0.033720-0 · Cited by 66
Short Communication: Effect of Exopolysaccharide Isolated from “Viili” on the Adhesion of Probiotics and Pathogens to Intestinal Mucus
Ruas‐Madiedo, P., Gueimonde, M., Reyes‐Gavilán, C. G. D. L., & Salminen, S. (2006) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(06)72307-4 · Cited by 65
Self-citation
Towards galactose accumulation in dairy foods fermented by conventional starter cultures: Challenges and strategies
Wu, Q., Cheung, C. K. W., & Shah, N. P. (2014) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2014.08.010 · Cited by 64
Anti-elastase and anti-collagenase potential of Lactobacilli exopolysaccharides on human fibroblast
Shirzad, ‪., Hamedi, J., Motevaseli, E., & Modarressi, M. H. (2018) · Artificial Cells Nanomedicine and Biotechnology · doi:10.1080/21691401.2018.1443274 · Cited by 67
Production, isolation and characterization of exopolysaccharides produced by Lactococcus lactis subsp. cremoris JFR1 and their interaction with milk proteins: Effect of pH and media composition
Hernandez, I. A., Hassan, A. H., Goff, H. D., de Orduña, R. M., & Corredig, M. (2008) · International Dairy Journal · doi:10.1016/j.idairyj.2008.06.008 · Cited by 63

Authors: Ruas-Madiedo, P., & de los Reyes-Gavilán, C. G.
Venue: Journal of Dairy Science
Publisher: American Dairy Science Association
Published: 2005-03
Volume: 88
Issue: 3
Pages: 843-856
ISSN: 0022-0302 (ISSN-L)
DOI: 10.3168/jds.s0022-0302(05)72750-8
OpenAlex: W2004400025
Cited by: 456
References: 135
Citations by year: 2026: 19 · 2025: 28 · 2024: 23 · 2023: 30 · 2022: 35 · 2021: 37 · 2020: 31 · 2019: 20 · 2018: 31 · 2017: 19 · 2016: 21 · 2015: 26 · 2014: 25 · 2013: 15 · 2012: 17
FWCI: 1.75
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 5069 d)
Locations: Journal of Dairy Science (publishedVersion)
PubMed (publishedVersion)
Funders: FEDER funds
Spanish “Plan Nacional de I+D+I”
“Fundación Príncipe de Asturias”
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
Keywords: exopolysaccharides, lactic acid bacteria, dairy fermentation, syneresis, dairy products
MeSH terms: Dairy Products, Fermentation, Food Microbiology, Lactobacillus, Polysaccharides, Bacterial, Viscosity, Probiotics, Organisms, Genetically Modified

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Invited Review: Methods for the Screening, Isolation, and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks