The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
The role of hypoxia-inducible factor-1α, aquaporin-4, and matrix metalloproteinase-9 in blood-brain barrier disruption and brain edema after traumatic brain injury: Laboratory investigation
Abstract
Object: The present study investigated the role of hypoxia-inducible factor-1α (HIF-1α), aquaporin-4 (AQP-4), and matrix metalloproteinase-9 (MMP-9) in blood-brain barrier (BBB) permeability alterations and brain edema formation in a rodent traumatic brain injury (TBI) model. Methods: The brains of adult male Sprague-Dawley rats (400–425 g) were injured using the Marmarou closed-head force impact model. Anti–AQP-4 antibody, minocycline (an inhibitor of MMP-9), or 2-methoxyestradiol (2ME2, an inhibitor of HIF-1α), was administered intravenously 30 minutes after injury. The rats were killed 24 hours after injury and their brains were examined for protein expression, BBB permeability, and brain edema. Expression of HIF-1α, AQP-4, and MMP-9 as well as expression of the vascular basal lamina protein (laminin) and tight junction proteins (zona occludens-1 and occludin) was determined by Western blotting. Blood-brain barrier disruption was assessed by FITC-dextran extravasation, and brain edema was measured by the brain water content. Results: Significant (p < 0.05) edema and BBB extravasations were observed following TBI induction. Compared with sham-operated controls, the injured animals were found to have significantly (p < 0.05) enhanced expression of HIF-1α, AQP-4, and MMP-9, in addition to reduced amounts (p < 0.05) of laminin and tight junction proteins. Edema was significantly (p < 0.01) decreased after inhibition of AQP-4, MMP-9, or HIF-1α. While BBB permeability was significantly (p < 0.01) ameliorated after inhibition of either HIF-1α or MMP-9, it was not affected following inhibition of AQP-4. Inhibition of MMP reversed the loss of laminin (p < 0.01). Finally, while inhibition of HIF-1α significantly (p < 0.05) suppressed the expression of AQP-4 and MMP-9, such inhibition significantly (p < 0.05) increased the expression of laminin and tight junction proteins. Conclusions: The data support the notion that HIF-1α plays a role in brain edema formation and BBB disruption via a molecular pathway cascade involving AQP-4 and MMP-9. Pharmacological blockade of this pathway in patients with TBI may provide a novel therapeutic strategy.
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Yang, Y., Estrada, E. Y., Thompson, J. F., Liu, W., & Rosenberg, G. A. (2007) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600375 · Cited by 1,060
Predominance of cellular edema in traumatic brain swelling in patients with severe head injuries
Marmarou, A., Signoretti, S., Fatouros, P. P., Portella, G., Aygok, G. A., & Bullock, M. R. (2006) · Journal of Neurosurgery · doi:10.3171/jns.2006.104.5.720 · Cited by 237
Park, E., Bell, J. D., Siddiq, I. P., & Baker, A. J. (2009) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2008.151 · Cited by 134
Mori, T., Wang, X., Aoki, T., & Lo, E. H. (2002) · Journal of Neurotrauma · doi:10.1089/089771502320914642 · Cited by 129
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Molecular pathophysiology of cerebral edema
Stokum, J. A., Gerzanich, V., & Simard, J. M. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x15617172 · Cited by 587
Matrix metalloproteinases as therapeutic targets for stroke
Yang, Y., & Rosenberg, G. A. (2015) · Brain Research · doi:10.1016/j.brainres.2015.04.024 · Cited by 246
Li, H., Sun, J., Wang, F., Ding, G., Chen, W., Fang, R., Yao, Y., Pang, M., Lu, Z.-Q., & Liu, J. (2016) · Brain Research · doi:10.1016/j.brainres.2016.03.031 · Cited by 152
The pericyte–glia interface at the blood–brain barrier
Giannoni, P., Badaut, J., Dargazanli, C., De Maudave, A. F., Klement, W., Costalat, V., & Marchi, N. (2018) · Clinical Science · doi:10.1042/cs20171634 · Cited by 81
Sabir, M., Gaudreault, P.-O., Freyburger, M., Massart, R., Blanchet-Cohen, A., Jaber, M., Gosselin, N., & Mongrain, V. (2015) · Brain, Behavior, and Immunity · doi:10.1016/j.bbi.2014.12.023 · Cited by 47
Metadata
- Authors
- Higashida, T., Kreipke, C. W., Rafols, J. A., Peng, C., Schafer, S., Schafer, P., Ding, J. Y., Dornbos, D., Li, X., Guthikonda, M., Rossi, N. F., & Ding, Y.
- Venue
- Journal of Neurosurgery
- Published
- 2011-01
- Volume
- 114
- Issue
- 1
- Pages
- 92-101
- ISSN
- 0022-3085 (ISSN-L)
- 1933-0693
- OpenAlex
- W2163862018
- Cited by
- 282
- References
- 4
- FWCI
- 10.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- Higashida, T., Kreipke, C. W., Rafols, J. A., Peng, C., Schafer, S., Schafer, P., Ding, J. Y., Dornbos, D., Li, X., Guthikonda, M., Rossi, N. F., & Ding, Y. (2011). The role of hypoxia-inducible factor-1α, aquaporin-4, and matrix metalloproteinase-9 in blood-brain barrier disruption and brain edema after traumatic brain injury: Laboratory investigation. Journal of Neurosurgery. https://doi.org/10.3171/2010.6.jns10207
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