CO2 – Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes
Open accessAbstract
Carbon dioxide formation mirrors the final carbon oxidation steps of aerobic metabolism in microbial and mammalian cells. As a consequence, [Formula: see text] dissociation equilibria arise in fermenters by the growing culture. Anaplerotic reactions make use of the abundant [Formula: see text] levels for refueling citric acid cycle demands and for enabling oxaloacetate-derived products. At the same time, CO2 is released manifold in metabolic reactions via decarboxylation activity. The levels of extracellular [Formula: see text] depend on cellular activities and physical constraints such as hydrostatic pressures, aeration, and the efficiency of mixing in large-scale bioreactors. Besides, local [Formula: see text] levels might also act as metabolic inhibitors or transcriptional effectors triggering regulatory events inside the cells. This review gives an overview about fundamental physicochemical properties of [Formula: see text] in microbial and mammalian cultures effecting cellular physiology, production processes, metabolic activity, and transcriptional regulation.
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Levels of CO2/HCO3− readily diffuse across cell membranes to moderate intracellular pH, act as metabolic inhibitors, may induce complex transcriptional responses, and signal other critical reactions.
“Besides the role of CO2 in affecting medium acid-base chemistry, levels of CO2/HCO3 − readily diffuse across cell membranes to moderate intracellular pH (Gutknecht et al., 1977), act as metabolic inhibitors, and may induce complex transcriptional responses (Cottier et al., 2012; Follonier et al., 2013), and signal other critical reactions (see, Blombach and Takors, 2015).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Blombach and Takors, 2015
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Flagged first, then by citations
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Research works printed but not linked: 1
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Metabolic engineering of Vibrio natriegens
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Yeast metabolic engineering for carbon dioxide fixation and its application
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Jahanian, A., Ramirez, J., & O’Hara, I. M. (2024) · Computers & Chemical Engineering · doi:10.1016/j.compchemeng.2024.108755 · Cited by 32 · see citing works
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Toward Best Practices for Controlling Mammalian Cell Culture Environments
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Self-citationCO 2 /HCO 3 − Accelerates Iron Reduction through Phenolic Compounds
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Self-citationA Carbonic Anhydrase Pseudogene Sensitizes Select Brucella Lineages to Low CO 2 Tension
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Enhanced CO 2 Capture-Desorption by Surface-Active Amine
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Shi, Y., Wan, Y., Sun, Y., Yang, J., Lu, Y., Xie, X., Pan, J., Wang, H., & Qu, H. (2024) · Biotechnology Journal · doi:10.1002/biot.202300495 · Cited by 13 · see citing works
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Single Cell Protein Production From Ethanol: Model‐Based Bioreactor Operation at Industrial Scale
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Ruminal CO2 Holdup Monitoring, Acidosis Might Be Caused by CO2 PoisoningPreprint
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Карбоксітерапія як патогенетично орієнтований підхід у лікуванні остеоартрозу колінного суглоба
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- Authors
- Blombach, B., & Takors, R.
- Affiliations
- University of Stuttgart (DE)
- Corresponding author
- Takors, Ralf
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2015-08-03
- Volume
- 3
- Pages
- 108-108
- ISSN
- 2296-4185 (ISSN-L)
- PMC
- PMC4522908
- OpenAlex
- W2157039001
- Cited by
- 65
- References
- 114
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 8 · 2023: 7 · 2022: 7 · 2021: 8 · 2020: 7 · 2019: 10 · 2018: 4 · 2017: 2 · 2016: 3
- FWCI
- 0.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- OPUS Publication Server of the University of Stuttgart (University of Stuttgart) (submittedVersion, other-oa)
- Universitätsbibliothek Stuttgart
- Funders
- Deutsche Forschungsgemeinschaft (TA 241/5-1, TA 241/5–2)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- carbon dioxide, anaplerotic reactions, TCA cycle, decarboxylation, hydrostatic pressure, mixing efficiency, metabolic regulation, transcriptional regulation, microbial fermentation, mammalian cell culture
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