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CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects
Open accessAbstract
Technologies and innovations are critical for addressing the future food system needs where genetic resources are an essential component of the change process. Advanced breeding tools like "genome editing" are vital for modernizing crop breeding to provide game-changing solutions to some of the "must needed" traits in agriculture. CRISPR/Cas-based tools have been rapidly repurposed for editing applications based on their improved efficiency, specificity and reduced off-target effects. Additionally, precise gene-editing tools such as base editing, prime editing, and multiplexing provide precision in stacking of multiple traits in an elite variety, and facilitating specific and targeted crop improvement. This has helped in advancing research and delivery of products in a short time span, thereby enhancing the rate of genetic gains. A special focus has been on food security in the drylands through crops including millets, teff, fonio, quinoa, Bambara groundnut, pigeonpea and cassava. While these crops contribute significantly to the agricultural economy and resilience of the dryland, improvement of several traits including increased stress tolerance, nutritional value, and yields are urgently required. Although CRISPR has potential to deliver disruptive innovations, prioritization of traits should consider breeding product profiles and market segments for designing and accelerating delivery of locally adapted and preferred crop varieties for the drylands. In this context, the scope of regulatory environment has been stated, implying the dire impacts of unreasonable scrutiny of genome-edited plants on the evolution and progress of much-needed technological advances.
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“Research is exploring the potential of multiplex-CRISPR gene editing constructs to accelerate genetic gains in underutilized crops, focusing on traits that enhance tolerance to abiotic stresses (Sharma et al., 2022).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Sharma et al., 2022
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Cited by
Sorted by citations
Genome editing in food and agriculture: from regulations to consumer perspectives
Atimango, A. O., Wesana, J., Kalule, S. W., Verbeke, W., & Steur, H. D. (2024) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2024.103127 · Cited by 48
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 55
Current trends and prospects in quinoa research: An approach for strategic knowledge areas
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Metadata
- Authors
- Sharma, K. K., Palakolanu, S. R., Bhattacharya, J., Shankhapal, A. R., & Bhatnagar-Mathur, P.
- Affiliations
- Energy and Resources Institute (IN)
- International Crops Research Institute for the Semi-Arid Tropics (IN)
- University of Nottingham (GB)
- Corresponding author
- Sharma, Kiran K.
- Venue
- Frontiers in Genetics
- Publisher
- Frontiers Media SA
- Published
- 2022-10-06
- Volume
- 13
- Pages
- 999207-999207
- ISSN
- 1664-8021 (ISSN-L)
- PMC
- PMC9582247
- OpenAlex
- W4303192285
- Cited by
- 24
- References
- 209
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 5 · 2023: 2 · 2022: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Genetics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Open Access Repository of ICRISAT (International Crops Research Institute for the Semi-Arid Tropics) (acceptedVersion)
- Rothamsted Repository (Rothamsted Repository) (acceptedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Consortium of International Agricultural Research Centers
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Agricultural Productivity and Crop Improvement
- Keywords
- CRISPR/Cas genome editing, rainfed agriculture, genetic gain, base editing, prime editing, multiplex genome editing, millet, Eragrostis tef, fonio, quinoa, Vigna subterranea, Cajanus cajan, cassava, stress tolerance, nutritional improvement, yield improvement, food security
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